Slc29a1 (NM_022880) Mouse Tagged ORF Clone

CAT#: MG207306

Slc29a1 (GFP-tagged) - Mouse solute carrier family 29 (nucleoside transporters), member 1 (Slc29a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc29a1
• Synonyms: 1200014D21Rik; AA407560; ENT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207306 representing NM_022880
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACCAGTCACCAGCCTCAGGACAGGTATAAGGCAGTATGGCTTATCTTCTTTGTGCTGGGCCTGG
  GGACACTGCTCCCCTGGAATTTTTTTATGACCGCAACCAAGTATTTCACAAACCGCCTGGACGTGTCCCA
  GAATGTGTCCTCGGACACTGATCAATCATGCGAAAGCACCAAGGCCTTGGCTGACCCCACAGTGGCCTTG
  CCAGCCCGGAGTTCTCTCAGTGCCATCTTCAACAATGTCATGACCCTGTGTGCCATGCTGCCCTTGCTGG
  TCTTCACCTGCCTCAACTCGTTTCTGCATCAGCGGATCTCTCAATCTGTTCGGATCTTGGGCAGCCTGCT
  GGCAATCCTGCTGGTATTCCTTGTCACTGCCGCCCTGGTGAAGGTGGAGATGGATGCTCTGACCTTCTTT
  GTCATCACCATGATCAAGATTGTGCTCATCAATTCATTTGGTGCCATTTTGCAAGCCAGCCTTTTTGGTC
  TGGCAGGTGTCCTGCCAGCCAACTACACAGCCCCCATCATGAGTGGCCAGGGCCTGGCTGGCTTCTTCAC
  CTCTGTCGCCATGATCTGTGCCATTGCCAGTGGTTCTGAGCTGTCAGAAAGCGCCTTTGGCTACTTCATC
  ACAGCCTGTGCAGTTGTCATTTTGGCCATCCTGTGCTACCTGGCTCTGCCTCGGACGGAATTCTATCGCC
  ATTACCTGCAGCTCAACCTTGCGGGGCCTGCAGAGCAGGAGACCAAGTTGGATCTCATAAGTAAAGGAGA
  GGAGCCAAAAGGAAGAAGAGAGGAATCTGGGGTGCCAGGCCCCAACTCTCCACCCACCAACAGAAACCAG
  TCTATCAAAGCCATACTTAAGAGTATCTGTGTCCCGGCTCTGTCTGTCTGCTTCATCTTCACGGTTACCA
  TTGGGTTGTTCCCTGCTGTGACTGCTGAGGTGGAATCCAGCATCGCAGGCACAAGTCCCTGGAAAAGCTA
  CTTCATTCCCGTGGCCTGTTTCTTGAATTTCAATGTCTTTGACTGGCTAGGCCGGAGCCTCACTGCTGTC
  TGCATGTGGCCTGGCCAGGATAGCCGCTGGCTGCCGGTTTTGGTCGCCTCGAGGATTGTGTTTGTTCCCC
  TGCTGATGCTCTGCAACGTGAAGGCTCGCCACTGCGGCGCGCAGCGGCACCACTTCGTCTTTAAGCATGA
  CGCCTGGTTCATCGCCTTCATGGCTGCCTTTGCCTTCTCCAATGGCTACCTCGCCAGCCTCTGCATGTGC
  TTCGGGCCCAAGAAAGTCAAACCAGCTGAGGCGGAGACAGCAGGAAACATCATGTCCTTCTTTCTGTGTC
  TGGGCCTGGCTCTGGGAGCTGTGTTGTCCTTCTTGTTAAGGGCACTTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207306 representing NM_022880
  Red=Cloning site Green=Tags(s)
  
  MTTSHQPQDRYKAVWLIFFVLGLGTLLPWNFFMTATKYFTNRLDVSQNVSSDTDQSCESTKALADPTVAL
  PARSSLSAIFNNVMTLCAMLPLLVFTCLNSFLHQRISQSVRILGSLLAILLVFLVTAALVKVEMDALTFF
  VITMIKIVLINSFGAILQASLFGLAGVLPANYTAPIMSGQGLAGFFTSVAMICAIASGSELSESAFGYFI
  TACAVVILAILCYLALPRTEFYRHYLQLNLAGPAEQETKLDLISKGEEPKGRREESGVPGPNSPPTNRNQ
  SIKAILKSICVPALSVCFIFTVTIGLFPAVTAEVESSIAGTSPWKSYFIPVACFLNFNVFDWLGRSLTAV
  CMWPGQDSRWLPVLVASRIVFVPLLMLCNVKARHCGAQRHHFVFKHDAWFIAFMAAFAFSNGYLASLCMC
  FGPKKVKPAEAETAGNIMSFFLCLGLALGAVLSFLLRALV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022880
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022880.2, NP_075018.1
• RefSeq Size: 2012
• RefSeq ORF: 1377
• Locus ID: 63959
• Gene Summary: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs). [UniProtKB/Swiss-Prot Function]