Nfs1 (NM_010911) Mouse Tagged ORF Clone

CAT#: MG207324

Nfs1 (GFP-tagged) - Mouse nitrogen fixation gene 1 (S. cerevisiae) (Nfs1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nfs1
• Synonyms: AA987187; m-Nfs1; m-Nfsl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207324 representing NM_010911
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGCTCTGTGGCTGGGAACATGCTGCTGCGAGCCGCTTGGAGGCGGGCGTCGTTGGCGGCTACCT
  CCTTGGCCCTGGGAAGGTCCTCGGTGCCCACCCGGGGACTGCGCCTGCGCGTTGTAGATCATGGCCCCCA
  TTCGCCTGTTCACTCAGAGGCAGAGGCAGTGCTGCGACCTCTCTACATGGACGTACAGGCCACCACTCCT
  CTGGATCCCAGAGTGCTTGATGCCATGCTCCCATACCTTGTCAACTACTATGGGAACCCTCATTCTCGGA
  CTCATGCATATGGCTGGGAGAGCGAGGCAGCCATGGAACGTGCTCGCCAGCAAGTAGCATCTCTGATTGG
  AGCTGATCCTCGGGAGATCATTTTCACTAGTGGAGCTACTGAGTCCAACAACATAGCAATTAAGGGAGTG
  GCCAGGTTCTACAGGTCAAGGAAAAAACACTTGGTCACAACCCAGACAGAACACAAATGTGTGCTGGATT
  CCTGCCGCTCCCTGGAAGCTGAGGGCTTTCGGGTCACTTACCTCCCTGTGCAGAAGAGCGGGATCATTGA
  CTTAAAGGAACTAGAGGCTGCCATCCAGCCAGACACCAGCCTGGTCTCCGTTATGACTGTGAACAATGAA
  ATTGGAGTAAAGCAACCAATTGCAGAAATAGGGCAGATTTGTAGTTCCAGAAAGGTGTACTTCCACACTG
  ATGCCGCCCAAGCGGTTGGGAAAATCCCACTTGACGTCAATGACATGAAGATTGATCTCATGAGCATCAG
  CGGTCACAAACTCTATGGCCCTAAAGGGGTTGGTGCTATCTATATCCGCCGTAGGCCCCGGGTACGTGTG
  GAGGCCCTACAGAGTGGAGGCGGGCAGGAGCGGGGTATGCGGTCTGGGACAGTGCCCACACCCTTGGTGG
  TGGGCCTGGGAGCTGCATGTGAGTTGGCACAACAAGAGATGGAATACGACCATAAGCGGATCTCAAAGTT
  AGCAGAGCGACTGGTACAGAACATAATGAAGAATCTTCCAGATGTGGTAATGAACGGGGACCCGAAGCAG
  CACTACCCTGGCTGTATCAACCTCTCCTTCGCGTATGTGGAAGGAGAGAGCCTGCTGATGGCCCTCAAGG
  ATGTGGCCTTGTCCTCAGGGAGTGCCTGCACTTCTGCATCATTGGAGCCCTCTTATGTCCTCAGAGCAAT
  TGGTACCGATGAGGATTTAGCACACTCCTCTATCAGGTTTGGCATCGGCCGCTTCACTACAGAAGAGGAG
  GTGGACTACACGGCGGAGAAGTGCATCCACCACGTGAAGCGCCTTCGAGAAATGAGTCCTCTCTGGGAGA
  TGGTCCAGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAGCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207324 representing NM_010911
  Red=Cloning site Green=Tags(s)
  
  MVGSVAGNMLLRAAWRRASLAATSLALGRSSVPTRGLRLRVVDHGPHSPVHSEAEAVLRPLYMDVQATTP
  LDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGV
  ARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNE
  IGVKQPIAEIGQICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRV
  EALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNGDPKQ
  HYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEE
  VDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010911
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010911.2, NP_035041.2
• RefSeq Size: 2053
• RefSeq ORF: 1380
• Locus ID: 18041