Pik3r3 (NM_181585) Mouse Tagged ORF Clone

CAT#: MG207362

Pik3r3 (GFP-tagged) - Mouse phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) (Pik3r3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pik3r3
• Synonyms: AA414954; p55; p55pik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207362 representing NM_181585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAATACGGTGTGGAGTATGGACCGCGATGACGCAGACTGGAGGGAGGTGATGATGCCCTATTCGA
  CAGAACTGATATTTTATATTGAAATGGATCCTCCAGCTCTTCCACCAAAGCCACCTAAGCCAATGACTCC
  AGCAGTCACAAATGGAATGAAGGACAGTTTCATTTCTCTTCAAGATGCAGAGTGGTACTGGGGAGATATT
  TCCAGGGAAGAGGTAAATGACAAATTGCGGGACATGCCAGATGGTACCTTCTTAGTTCGTGATGCCTCAA
  CGAAAATGCAGGGGGATTATACATTGACTTTGAGGAAGGGAGGAAATAATAAATTAATAAAGATCTATCA
  TCGGGATGGTAAATATGGCTTCTCTGAGCCCCTGACGTTTACTTCTGTGGTGGAGCTTATTAACCACTAC
  CACCACGAGTCTCTCGCTCAGTACAATCCCAAACTCGACGTGAAGCTGACGTACCCAGTATCCAGATTCC
  AACAGGATCAGTTGGTAAAAGAAGATAACATTGATGCAGTAGGTAAAAATCTGCAGGAGTTCCACTCTCA
  GTATCAGGAGAAGAGCAAAGAGTATGACAGGCTGTATGAAGAGTACACAAGGACATCACAGGAAATACAA
  ATGAAGAGGACTGCCATTGAAGCCTTTAATGAAACAATTAAAATATTTGAGGAGCAGTGTCATACCCAAG
  AACAACACAGTAAAGACTATATCGAGCGCTTTCGCAGAGAGGGGAATGAGAAGGAGATCGAGCGAATTAT
  GATGAATTATGATAAATTGAAATCACGTCTTGGTGAGATTCATGATAGCAAACTGCGTCTTGAGCAGGAC
  TTGAAGAAACAAGCTTTGGACAACCGGGAAATAGATAAAAAAATGAATAGCATCAAACCCGACTTGATCC
  AGCTGCGTAAGATCCGGGATCAGCACCTTGTATGGCTCAATCACAGAGGAGTGAGGCAGAGGCGCCTGAA
  TGCCTGGCTGGGGATCAAGAATGAGGACTCAGATGAAAGCTATTTTATCAATGAGGAAGATGAGAACCTG
  CCGCATTATGATGAGAAAACCTGGTTTGTGGAGGATATCAACCGAGTACAAGCAGAGGACTTGCTTTATG
  GGAAACCAGATGGTGCATTCTTAATTCGTGAGAGTAGCAAGAAAGGATGTTACGCTTGTTCTGTGGTTGC
  AGACGGGGAAGTGAAGCACTGTGTCATCTACAGCACGGCTCGAGGATATGGCTTTGCAGAACCCTACAAC
  CTGTACAGCTCACTGAAGGAGCTGGTGCTCCATTACCAGCAGACATCCCTGGTTCAGCACAACGACTCCC
  TCAACGTCAGGCTCGCCTACCCTGTCCATGCACAGATGCCTACGCTCTGCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207362 representing NM_181585
  Red=Cloning site Green=Tags(s)
  
  MYNTVWSMDRDDADWREVMMPYSTELIFYIEMDPPALPPKPPKPMTPAVTNGMKDSFISLQDAEWYWGDI
  SREEVNDKLRDMPDGTFLVRDASTKMQGDYTLTLRKGGNNKLIKIYHRDGKYGFSEPLTFTSVVELINHY
  HHESLAQYNPKLDVKLTYPVSRFQQDQLVKEDNIDAVGKNLQEFHSQYQEKSKEYDRLYEEYTRTSQEIQ
  MKRTAIEAFNETIKIFEEQCHTQEQHSKDYIERFRREGNEKEIERIMMNYDKLKSRLGEIHDSKLRLEQD
  LKKQALDNREIDKKMNSIKPDLIQLRKIRDQHLVWLNHRGVRQRRLNAWLGIKNEDSDESYFINEEDENL
  PHYDEKTWFVEDINRVQAEDLLYGKPDGAFLIRESSKKGCYACSVVADGEVKHCVIYSTARGYGFAEPYN
  LYSSLKELVLHYQQTSLVQHNDSLNVRLAYPVHAQMPTLCR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181585
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_181585.5, NP_853616.1
• RefSeq Size: 5004
• RefSeq ORF: 1386
• Locus ID: 18710
• Gene Summary: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. [UniProtKB/Swiss-Prot Function]