Dbt (NM_010022) Mouse Tagged ORF Clone

CAT#: MG207721

Dbt (GFP-tagged) - Mouse dihydrolipoamide branched chain transacylase E2 (Dbt)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dbt
• Synonyms: BCKAD-E2; D3Wsu60e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207721 representing NM_010022
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGCGCGTGTACTGAGGACCTGGAGCCAGAACGCTGTCAGACTGACCTGTGTTCGCTATTTTC
  AGACATTTAATAGCGCCCGTGTTTTGAAGCCAAAATGTGTGTGCTCTGTGGGTTATCCATTGTTCAAGTA
  CAGTCAGCCACGTCACTCCCTGAGAACAGCGGCCGTGCTCCAGGGACAGGTTGTTCAGTTCAAACTCTCC
  GACATCGGAGAAGGAATTCGAGAAGTAACTATTAAAGAATGGTATGTAAAAGAAGGAGATACGGTGTCTC
  AGTTTGACAGCATCTGTGAAGTTCAAAGTGACAAGGCCTCTGTCACCATCACCAGCCGTTACGATGGCAT
  CATTAAGAGGCTCTATTACAATCTAGATGATATCGCTTATGTGGGGAAACCACTGATCGACATTGAGACA
  GAAGCTTTAAAGGATTCAGAGGAAGATGTTGTTGAAACCCCCGCTGTGTCCCATGATGAGCACACTCACC
  AAGAGATAAAAGGCCAGAAAACACTAGCAACGCCTGCAGTGCGTCGGCTGGCAATGGAAAACAATATTAA
  GCTGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTAAAGAAGATATTCTCAGCTTTTTGGAA
  AAGCAGACAGGAGCCATACTGCCTCCCTCACCAAAATCTGAAATCACACCACCTCCACCACAGCCCAAAG
  ACAGGACCTTTCCCACGCCGATAGCAAAGCCTCCAGTGTTCACAGGCAAAGACCGAACAGAGCCAGTGAC
  AGGCTTCCAGAAGGCAATGGTGAAGACCATGTCTGCAGCCCTGAAGATTCCCCACTTCGGGTATTGTGAT
  GAGATTGACCTTACTCAACTGGTTAAGCTTCGAGAAGAGCTGAAACCTGTTGCTTTGGCGCGTGGAATCA
  AACTCTCCTTCATGCCCTTCTTCTTAAAGGCTGCTTCTCTGGGACTCCTGCAGTTTCCTATCCTCAATGC
  CTCCGTGGACGAGAACTGCCAGAACATCACCTACAAGGCTTCTCACAACATTGGGATAGCAATGGACACT
  GAGCTGGGGTTAATTGTCCCCAATGTGAAGAACGTTCAGGTCCGCTCTGTATTTGAGATCGCCATGGAAC
  TGAACCGCCTCCAGAAACTGGGCTCTTCCGGTCAGCTCGGTACCACCGACCTTACAGGAGGGACATTTAC
  TCTTTCCAACATTGGATCAATCGGTGGAACCTATGCCAAGCCAGTGATATTGCCACCGGAAGTAGCCATT
  GGTGCCCTGGGAGCGATTAAGGCTCTTCCCCGATTTGACCAGAAAGGAGACGTTTATAAAGCACAGATAA
  TGAATGTGAGCTGGTCAGCGGATCACAGGGTCATCGATGGGGCCACCATGTCACGCTTCTCTAATTTGTG
  GAAATCCTACTTAGAAAACCCAGCGTTTATGCTGCTGGATCTGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207721 representing NM_010022
  Red=Cloning site Green=Tags(s)
  
  MAAARVLRTWSQNAVRLTCVRYFQTFNSARVLKPKCVCSVGYPLFKYSQPRHSLRTAAVLQGQVVQFKLS
  DIGEGIREVTIKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGIIKRLYYNLDDIAYVGKPLIDIET
  EALKDSEEDVVETPAVSHDEHTHQEIKGQKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILSFLE
  KQTGAILPPSPKSEITPPPPQPKDRTFPTPIAKPPVFTGKDRTEPVTGFQKAMVKTMSAALKIPHFGYCD
  EIDLTQLVKLREELKPVALARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDT
  ELGLIVPNVKNVQVRSVFEIAMELNRLQKLGSSGQLGTTDLTGGTFTLSNIGSIGGTYAKPVILPPEVAI
  GALGAIKALPRFDQKGDVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010022
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010022.1, NP_034152.1
• RefSeq Size: 2664
• RefSeq ORF: 1449
• Locus ID: 13171
• Gene Summary: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProtKB/Swiss-Prot Function]