Sufu (NM_001025391) Mouse Tagged ORF Clone

CAT#: MG207758

Sufu (GFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sufu
• Synonyms: Su(fu)
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207758 representing NM_001025391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGCGGCCTAGCGTCGCCCCCGGTCCCGCCGCGCCCCCGGCCTCTGGCCCTAGTGCCCCTC
  CGGCCTTTGCTTCACTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAGTGTCGCCGCCTCTACCCTGA
  CCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTACTGGTTGGGTGGTCCGGACCCCTTGGACTAT
  GTTAGCATGTACAGGAACATGGGGAGTCCTTCTGCCAACATCCCTGAGCACTGGCACTACATCAGCTTTG
  GCCTGAGTGATCTCTATGGTGACAACAGAGTCCATGAGTTTACAGGAACAGACGGACCAAGTGGATTTGG
  CTTTGAGTTGACGTTTCGTCTGAAGAGAGAAACTGGGGAGTCTGCCCCACCAACATGGCCAGCAGAGCTG
  ATGCAGGGCCTAGCCCGATATGTCTTCCAGTCAGAGAACACCTTCTGTAGCGGGGACCATGTGTCTTGGC
  ACAGCCCTTTGGATAACAGTGAGTCAAGAATTCAGCACATGCTGCTGACGGAGGACCCACAGATGCAGCC
  TGTGCGGACACCCTTTGGGGTAGTGACTTTCCTCCAGATTGTTGGTGTCTGCACTGAGGAGTTACATTCA
  GCCCAACAGTGGAACGGGCAGGGCATCCTGGAACTACTACGGACAGTGCCCATTGCTGGCGGTCCCTGGC
  TGATAACTGACATGCGGCGGGGAGAAACCATATTTGAGATCGATCCGCACCTGCAAGAGAGAGTTGACAA
  AGGCATTGAGACAGACGGTTCTAACCTGAGCGGCGTCAGTGCCAAGTGTGCCTGGGATGACCTCAGCCGG
  CCTCCGGAGGATGAAGAGGATAGCCGGAGCATCTGCCTCGGCACACAGCCTCGGAGGCTGTCTGGCAAAG
  ACACAGAGCAGATCCGGGAGACCCTGAGGCGGGGACTGGAGATTAACAGCAAACCTGTCCTTCCACCAAT
  CAATTCTCAGCGACAGAACGGCCTCACCCACGACAGGGCTCCGAGCCGCAAGGACAGTTTGGGCAGCGAC
  AGCTCCACGGCCATCATCCCCCACGAGCTGATCCGCACACGGCAGCTCGAGAGCGTGCATCTAAAATTTA
  ACCAAGAGTCGGGAGCCCTCATCCCTCTCTGCCTAAGGGGCAGACTCCTACATGGCCGGCACTTCACCTA
  CAAGAGTATCACAGGCGACATGGCCATCACGTTTGTGTCCACGGGAGTGGAAGGCGCCTTTGCCACTGAG
  GAACACCCGTATGCAGCTCACGGACCCTGGTTACAAATTCTGTTGACAGAAGAGTTTGTAGAGAAGATGT
  TGGAGGACTTAGAAGATCTAACCTCTCCAGAGGAATTTAAACTTCCCAAAGAGTACAGCTGGCCTGAGAA
  GAAACTCAAAGTGTCCATTCTCCCCGACGTGGTGTTCGACAGTCCACTGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207758 representing NM_001025391
  Red=Cloning site Green=Tags(s)
  
  MAELRPSVAPGPAAPPASGPSAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDY
  VSMYRNMGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAEL
  MQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVRTPFGVVTFLQIVGVCTEELHS
  AQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSR
  PPEDEEDSRSICLGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINSQRQNGLTHDRAPSRKDSLGSD
  SSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATE
  EHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025391
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025391.1, NP_001020562.1
• RefSeq Size: 2356 bp
• RefSeq ORF: 1455 bp
• Locus ID: 24069
• Cytogenetics: 19 38.85 cM