Pepd (NM_008820) Mouse Tagged ORF Clone

CAT#: MG207914

Pepd (GFP-tagged) - Mouse peptidase D (Pepd)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pepd
• Synonyms: dal; Pep-4; Pep4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207914 representing NM_008820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCACTGTGCGCCCTTCCTTCTCCCTGGGGAATGAGACCCTGAAGGTGCCACTGGCACTATTCG
  CCCTGAACAGGCAGCGCCTGTGTGAGAGGCTGAGGAAGAATGGAGCTGTACAGGCCGCCTCAGCTGTCGT
  GTTGCAGGGCGGGGAAGAGATGCAACGGTACTGCACCGATACCTCCATCATCTTCCGCCAGGAGTCCTTC
  TTTCACTGGGCCTTCGGTGTCGTCGAGTCAGGCTGCTATGGTGTCATCGATGTGGACACTGGAAAATCAA
  CCCTGTTTGTGCCCAGGCTTCCTGATAGCTACGCCACCTGGATGGGAAAGATCCATTCCAAGGAGTATTT
  CAAGGAGAAGTACGCTGTGGATGATGTCCAGTACACGGATGAGATTGCCAGTGTCCTGACCTCACGGAAC
  CCCTCTGTCCTCCTCACTTTGCGCGGTGTCAACACAGACAGTGGCAGTGTCTGCAGGGAGGCCTCCTTCG
  AGGGCATTAGCAAGTTCAATGTCAACAACACGATTCTTCACCCAGAGATCGTGGAATGCCGAGTGTTCAA
  GACAGACATGGAACTAGAGGTTTTGCGCTACACAAATCGGATCTCCAGCGAGGCCCACCGTGAGGTAATG
  AAGGCTGTAAAAGTGGGGATGAAGGAATACGAGATGGAAAGCCTGTTCCAGCATTATTGTTACTCTAGGG
  GCGGCATGCGCCACACCTCTTACACCTGTATCTGCTGTAGCGGTGAGAACGCGGCCGTGCTGCACTATGG
  CCATGCTGGAGCCCCCAATGACCGGACGATCAAGGATGGAGATATTTGCCTGTTCGACATGGGTGGCGAG
  TACTACTGCTTTGCTTCTGACATCACCTGCTCCTTCCCAGCCAACGGCAAGTTCACAGAGGACCAGAAGG
  CCATCTACGAAGCAGTGCTCAGGAGCTGCAGAACTGTCATGAGCACTATGAAGCCAGGGGTCTGGTGGCC
  TGATATGCATCGGCTGGCTGACCGTATCCACCTGGAGGAACTGGCACGCATTGGCCTCCTGAGTGGCAGT
  GTGGATGCCATGCTCCAGGTCCACCTGGGAGCAGTATTCATGCCACATGGACTTGGCCACTTTCTGGGCC
  TAGATGTGCACGACGTGGGAGGCTACCCTGAGGGTGTAGAGCGCATCGATGAACCTGGCCTGCGAAGCCT
  GCGTACGGCAAGGCACCTGGAGCCGGGCATGGTGCTGACTGTGGAGCCAGGCATCTACTTCATTGACCAC
  CTCTTGGACCAGGCCCTGGCAGACCCGGCCCAAGCCTGCTTCTTTAACCAAGAGGTCCTGCAGCGCTTCC
  GCAACTTCGGTGGGGTGCGGATCGAGGAAGATGTTGTCGTGACTGACAGCGGCATGGAGCTGCTGACATG
  TGTACCCCGGACTGTGGAGGAAATCGAAGCGTGCATGGCAGGCTGCGACAAGGCATCTGTGCCCTTCTCT
  GGCCAAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207914 representing NM_008820
  Red=Cloning site Green=Tags(s)
  
  MASTVRPSFSLGNETLKVPLALFALNRQRLCERLRKNGAVQAASAVVLQGGEEMQRYCTDTSIIFRQESF
  FHWAFGVVESGCYGVIDVDTGKSTLFVPRLPDSYATWMGKIHSKEYFKEKYAVDDVQYTDEIASVLTSRN
  PSVLLTLRGVNTDSGSVCREASFEGISKFNVNNTILHPEIVECRVFKTDMELEVLRYTNRISSEAHREVM
  KAVKVGMKEYEMESLFQHYCYSRGGMRHTSYTCICCSGENAAVLHYGHAGAPNDRTIKDGDICLFDMGGE
  YYCFASDITCSFPANGKFTEDQKAIYEAVLRSCRTVMSTMKPGVWWPDMHRLADRIHLEELARIGLLSGS
  VDAMLQVHLGAVFMPHGLGHFLGLDVHDVGGYPEGVERIDEPGLRSLRTARHLEPGMVLTVEPGIYFIDH
  LLDQALADPAQACFFNQEVLQRFRNFGGVRIEEDVVVTDSGMELLTCVPRTVEEIEACMAGCDKASVPFS
  GQK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008820
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008820.1, NP_032846.1
• RefSeq Size: 1876
• RefSeq ORF: 1482
• Locus ID: 18624
• Gene Summary: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because of the high level of iminoacids in collagen. [UniProtKB/Swiss-Prot Function]