Gria2 (BC048248) Mouse Tagged ORF Clone

CAT#: MG207939

Gria2 (GFP-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (cDNA clone MGC:54603 IMAGE:6494013)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gria2
• Synonyms: GluR-B, short form
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207939 representing BC048248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT
  CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG
  GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT
  TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGCGTCTATGCGATTTTTGGGTTTTACGACA
  AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTGCATGTATCCTTCATCACACCAAGCTTCCC
  AACAGATGGCACGCATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT
  GAGTACTACCAATGGGATAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG
  TGCTGGATTCTGCTGCGGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATTAACAATGACAA
  GAAAGATGAGACCTACAGATCACTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTTGAC
  TGCGAAAGGGATAAAGTCAATGACATTGTGGACCAGGTTATTACCATTGGAAAGCATGTTAAAGGGTACC
  ATTATATCATTGCAAATCTGGGATTTACTGATGGAGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT
  CTCTGGATTTCAGATTGTAGACTACGACGACTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTC
  GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACTTATGATGCTG
  TCCAAGTGATGACTGAAGCATTCCGCAATCTTCGGAAGCAGAGGATTGAAATCTCCAGGAGAGGAAATGC
  AGGAGATTGTTTGGCCAACCCAGCTGTGCCTTGGGGACAAGGCGTGGAAATAGAAAGGGCCCTCAAGCAG
  GTTCAAGTTGAAGGTCTCTCTGGAAATATAAAATTTGACCAGAACGGAAAACGAATAAACTACACAATTA
  ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT
  CACCCTAACCGAGCTCCCCTCTGGAAATGACACATCTGGGCTTGAAAACAAAACTGTGGTTGTCACCACA
  ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTATGAGG
  GCTACTGTGTTGACTTAGCTGCAGAAATTGCCAAACATTGTGGATTCAAGTACAAGCTGACTATTGTTGG
  GGATGGCAAGTATGGGGCCAGGGATGCAGACACCAAAATTTGGAATGGTATGGTTGGAGAACTTGTATAT
  GGGGTAAGTTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207939 representing BC048248
  Red=Cloning site Green=Tags(s)
  
  MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANS
  FAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLI
  EYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILD
  CERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTL
  EEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQ
  VQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTT
  ILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVY
  GVSF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC048248
• ORF Size: 1484 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC048248, AAH48248
• RefSeq Size: 4799
• RefSeq ORF: 1484
• Locus ID: 14800
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]