Gpt (NM_182805) Mouse Tagged ORF Clone

CAT#: MG207958

Gpt (GFP-tagged) - Mouse glutamic pyruvic transaminase 1, soluble (Gpt1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gpt
• Synonyms: 1300007J06Rik; 2310022B03Rik; ALT; ALT1; Gpt-1; Gpt1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207958 representing NM_182805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACAAAGGAATGACCGGATCCAGGCTTCAAGGAATGGACTGAAGGGGAAGGTGCTAACTCTGG
  ATACCATGAACCCATGTGTGCGGAGGGTGGAGTATGCAGTCCGAGGCCCCATCGTGCAACGTGCCTTGGA
  GCTGGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGCCTTTTACTGAGGTTATCCGTGCCAATATTGGGGAT
  GCACAAGCCATGGGGCAGAGACCCATCACCTTCTTCCGCCAGGTCCTGGCCCTCTGTGTCTACCCCAATC
  TTCTGAGCAGTCCGGACTTCCCAGAGGATGCCAAGAGAAGGGCAGAACGCATCTTGCAGGCATGCGGGGG
  CCACAGCCTGGGTGCCTATAGCATTAGCTCTGGAATCCAGCCGATTCGGGAGGATGTGGCGCAATATATT
  GAGAGGAGAGACGGAGGCATCCCTGCAGACCCGAACAACATATTTCTGTCCACAGGGGCCAGCGATGCCA
  TCGTGACCATGCTCAAGCTGCTGGTAGCCGGCGAGGGCCGTGCGCGAACCGGTGTACTCATTCCCATTCC
  TCAGTACCCACTGTACTCAGCTGCGCTGGCTGAGCTGGACGCCGTGCAAGTGGACTACTACCTGGACGAA
  GAGCGCGCCTGGGCTCTTGACATCGCTGAGCTGCGGCGCGCTCTGTGCCAGGCACGTGACCGCTGCTGCC
  CTCGAGTACTATGCGTCATCAACCCCGGCAACCCCACGGGGCAGGTGCAGACCCGTGAATGCATCGAGGC
  CGTAATCCGCTTTGCTTTCGAAGAGGGACTCTTCCTGATGGCTGATGAGGTATACCAAGACAATGTATAT
  GCCGAGGGCTCTCAGTTCCATTCATTCAAGAAGGTGCTCACGGAGATGGGGCCACCATATGCCACGCAGC
  AGGAGCTCGCGTCTTTCCACTCAGTCTCTAAGGGCTACATGGGCGAGTGCGGGTTTCGTGGTGGCTATGT
  GGAAGTGGTAAACATGGATGCCGAGGTGCAGAAACAGATGGCGAAACTGATGAGCGTGCGGTTGTGTCCA
  CCAGTGCCGGGCCAGGCTTTGATGGGCATGGTGGTCAGTCCGCCAACCCCCTCGGAGCCGTCCTTCAAGC
  AGTTTCAAGCAGAGAGGCAGGAGGTGCTGGCTGAACTGGCAGCCAAGGCTAAACTCACGGAGCAGGTCTT
  CAACGAGGCCCCCGGGATCCGCTGCAACCCGGTGCAGGGCGCTATGTATTCCTTCCCTCAAATTCAGCTG
  CCTTTGAAAGCAGTGCAGCGTGCGCAGGACCTGGGCCTGGCCCCTGACATGTTCTTCTGTCTGTGCCTCC
  TGGAAGAGACTGGCATCTGCGTTGTGCCTGGGAGTGGCTTTGGGCAGCAGGAGGGCACCTATCATTTCCG
  GATGACCATTCTGCCCCCCATGGAGAAACTGCGGGTGCTGCTGGAGAAACTGAGGCACTTCCATGCTAAA
  TTCACTCATGAGTACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207958 representing NM_182805
  Red=Cloning site Green=Tags(s)
  
  MASQRNDRIQASRNGLKGKVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGD
  AQAMGQRPITFFRQVLALCVYPNLLSSPDFPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYI
  ERRDGGIPADPNNIFLSTGASDAIVTMLKLLVAGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDE
  ERAWALDIAELRRALCQARDRCCPRVLCVINPGNPTGQVQTRECIEAVIRFAFEEGLFLMADEVYQDNVY
  AEGSQFHSFKKVLTEMGPPYATQQELASFHSVSKGYMGECGFRGGYVEVVNMDAEVQKQMAKLMSVRLCP
  PVPGQALMGMVVSPPTPSEPSFKQFQAERQEVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPQIQL
  PLKAVQRAQDLGLAPDMFFCLCLLEETGICVVPGSGFGQQEGTYHFRMTILPPMEKLRVLLEKLRHFHAK
  FTHEYS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182805
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_182805.1, NP_877957.1
• RefSeq Size: 1784
• RefSeq ORF: 1491
• Locus ID: 76282