Adpgk (NM_028121) Mouse Tagged ORF Clone

CAT#: MG207965

Adpgk (GFP-tagged) - Mouse ADP-dependent glucokinase (Adpgk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adpgk
• Synonyms: 2610017G09Rik; Adp-gk; C77577
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207965 representing NM_028121
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGTGGCGCGGCTCTGCGTGCGCCGGCTTCCTGGCGCTGGCCGTGGGCTGCGTGTTCCTGCTGG
  AGCCGGAGCTGCCGGGCACGGCGCTGCGCTCGCTCTGGAGCTCCCTGAGGCTGGGACCCGCGCCAGTGCC
  CGTGGGACCTCTGTCCCCGGAGAGCCGGCTGGCAGCCGCTTGGGACGCCCTCATCGCGCAGCCCGCCCGG
  CGCTGGCGCCGCGTGGCTGTGGGAGTCAACGCCTGTGTGGATGTGGTAATCTCTGGCGTGAAACTCCTGC
  AGGCCCTCGGCCTCAGTCCTGGGAGTGGAAAAGATCATGCTATTCTACACTCGAGAAGTGATCTGGAAGA
  AGCCTTCCTCTACTTCATGGGGAAGGGAGCCGCTGCTGAGCGCTTCTTCAGTGATAAAGAGACCTTTCAT
  GACATTGCCCAGGCTGCATCTGAGTTTCCAGGAGCTCAGCACTATGTAGGAGGAAATGCTGCTTTAATTG
  GACAGAGGTTTGCCGCCAACACAGACTTAAAGGTTCTTCTTTGTGGTCCAATTGGCCCAAAACTGCATGA
  ACTTCTTGATGACAATGTGTTTGTTCCACCAGAGTCACTGCAGGAAGAGGATGAGTTCCACCTTATTTTA
  GAGTACCTGGCAGGGGAGGAGTGGGGCCCGTTTAAAGCTCCCCATGCCAACCGCTTCATCTTCTCTCACG
  ACCTCTCCAACGGGGCCATGAATATGCTGGAGGTGTTTGTGTCTAGCCTGGAGGAGTTCCAGCCTGACCT
  GGTGGTCCTTTCTGGATTGCACATGATGGAGGGACAAAGCAAGGAGCTCCAGAGGAAGAGGCTCTTGGAG
  GTTGTGACCGCCATTTCTGACATCCCCACTGGTATTCCAGTTCACCTAGAGCTGGCCAGTATGACCAACA
  GGGAGCTCATGAGCAGTATTGTGCATCAGGTCTTTCCCGCGGTGGCTTCTCTTGGGCTGAATGAACAGGA
  GCTGCTGTTTCTCAGCCAGTCGGCATCTGGACCCCATTCTTCTCTCTCCTCCTGGGATGGTGTTCCTGAT
  GTGGGCATGGTCAGTGACATCCTCTTCTGGATCTTGAAAGAACATGGAAGGAGTGAAAACAGATCCTCGG
  ATCTCACCAGGATCCATTTCCACACGCTGGTCTACCACATTCTGGCAACTGTGGACGGACACTGGGCCAA
  CCAGCTCGCAGCCGTAGCTGCGGGAGCTCGTGTAGCTGGGACACAAGCCTGTGCCACAGAAACCATAGAC
  ACCAACCGAGTGTCTCTAAGAGCACCTCAGGAGTTTACGACATCCCATTTGGAATCCGGTTCCAGGATTG
  TATTGAACCCAGATAAGCCAGTGGTGGAATGGCACAGGGAGGGAATAACTTTCCACTTCACACCTGTGTT
  GGTGTGTAAAGACCCCGTTCGAACTGTAGGCCTTGGTGATGCCATTTCCGCTGAAGGCCTCTTCTATTCA
  GAAGCACGCCCTGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207965 representing NM_028121
  Red=Cloning site Green=Tags(s)
  
  MALWRGSACAGFLALAVGCVFLLEPELPGTALRSLWSSLRLGPAPVPVGPLSPESRLAAAWDALIAQPAR
  RWRRVAVGVNACVDVVISGVKLLQALGLSPGSGKDHAILHSRSDLEEAFLYFMGKGAAAERFFSDKETFH
  DIAQAASEFPGAQHYVGGNAALIGQRFAANTDLKVLLCGPIGPKLHELLDDNVFVPPESLQEEDEFHLIL
  EYLAGEEWGPFKAPHANRFIFSHDLSNGAMNMLEVFVSSLEEFQPDLVVLSGLHMMEGQSKELQRKRLLE
  VVTAISDIPTGIPVHLELASMTNRELMSSIVHQVFPAVASLGLNEQELLFLSQSASGPHSSLSSWDGVPD
  VGMVSDILFWILKEHGRSENRSSDLTRIHFHTLVYHILATVDGHWANQLAAVAAGARVAGTQACATETID
  TNRVSLRAPQEFTTSHLESGSRIVLNPDKPVVEWHREGITFHFTPVLVCKDPVRTVGLGDAISAEGLFYS
  EARPD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028121
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028121.2, NP_082397.1
• RefSeq Size: 2499
• RefSeq ORF: 1491
• Locus ID: 72141
• Gene Summary: Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency. [UniProtKB/Swiss-Prot Function]