Pdhx (NM_175094) Mouse Tagged ORF Clone

CAT#: MG208036

Pdhx (GFP-tagged) - Mouse pyruvate dehydrogenase complex, component X (Pdhx)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pdhx
• Synonyms: AI481367; E3bp; Pdx1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208036 representing NM_175094
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCCTGGAGGCTTCACTGTAACCAGCCGCTGCTTCGGTACCTTCTGGGATTTAGCAGCCGTC
  GAAGCCTGGGACTGGCTCAGGGTGCTGCTGCTTGGCCTGTCGACCGTGGAGCAAGTTGGAGGTGGTTTCA
  CAGTACTCAGCTGCTGCAGGCGGATCCTATTAAGGTCCTAATGCCATCACTGTCTCCTACGATGGAGCAA
  GGGAACATTGTGAAATGGCTGAGAAAGGAAGGTGAAGCTGTGAGTGCTGGAGACTCGTTATGTGAAATTG
  AGACGGACAAGGCTGTGGTCACCTTAGATGCAAACGATGATGGCATCTTAGCCAAGATAGTGGTTGAGGA
  AGGTGCTAAAAATATACAGCTGGGTTCACTCATTGCTTTGATGGTTGAAGAAGGGGAAGATTGGAAACAG
  GTGGAAATTCCCAAGGATGTCAGTGCTCCACCTCCAGTCTCAAAACCACCAGCTCCTACTCAGCCCTCTC
  CACAGCCGCAGATTCCTTGTCCCGCCAGGAAGGAGCACAAAGGGACGGCACGGTTCCGCTTAAGTCCAGC
  TGCCCGCAACATTCTGGAAAAACATTCATTGGACGCCAGCCAGGGCACAGCCACAGGCCCTCGCGGGATC
  TTCACTAAAGAAGATGCACTCAAGTTAGTCGAGCTGAAACAGATGGGCAAGATTACTGAATCCAGACCAG
  CCTCAGCTCCTCCACCCTCTCTCTCAGCATCTGTGCCTCCTCAGGCTACAGCCGGGCCATCCTATCCTCG
  GCCAATGACCCCACCAGTATCCATTCCTGGGCAACCGAATGCAGCGGGTACATTCACCGAAATTCCTGCT
  AGCAATATTCGAAGAGTTATCGCTAAGAGGTTAACTGAATCAAAAAGTACTGTGCCTCATGCCTATGCTA
  CTGCTGACTGTGACCTTGGCGCAGTTCTAAAGGTTAGACGAGATCTGGTCAAAGATGACATTAAAGTCTC
  AGTAAATGACTTTATCATCAGAGCAGCAGCTGTTACTCTTAAACAAATGCCTGGGGTTAATGTCACCTGG
  GATGGAGAAGGCCCAAAGCAGCTGCCCTCTGTTGACATTTCAGTGGCTGTGGCAACAGATAAAGGTTTAA
  TTACACCAATCATAAAAGATGCAGCCGCTAAGGGCATTCAGGAGATCGCTGACTCTGTAAAGGTTCTGTC
  GAAGAAAGCACGGGATGGGAAATTGATGCCAGAGGAGTACCAAGGAGGATCTTTTAGTATTTCCAACTTG
  GGGATGTTTGGCATCGATGAGTTCGCTGCAGTGATTAACCCGCCTCAGGCCTGTATCTTGGCCGTTGGGA
  GATTCCGCCCCGTGCTGAAGCTGACGGAGGATGAAGAGGGGAACCCTCAACTGCAGCAGCACCAGCTTAT
  AACGGTCACAATGTCAAGTGACAGCCGAGTGGTTGATGATGAACTAGCTACCAGGTTTCTTGAAACTTTC
  AAAGCAAACCTAGAGAATCCCATGCGACTTGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208036 representing NM_175094
  Red=Cloning site Green=Tags(s)
  
  MAASWRLHCNQPLLRYLLGFSSRRSLGLAQGAAAWPVDRGASWRWFHSTQLLQADPIKVLMPSLSPTMEQ
  GNIVKWLRKEGEAVSAGDSLCEIETDKAVVTLDANDDGILAKIVVEEGAKNIQLGSLIALMVEEGEDWKQ
  VEIPKDVSAPPPVSKPPAPTQPSPQPQIPCPARKEHKGTARFRLSPAARNILEKHSLDASQGTATGPRGI
  FTKEDALKLVELKQMGKITESRPASAPPPSLSASVPPQATAGPSYPRPMTPPVSIPGQPNAAGTFTEIPA
  SNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRRDLVKDDIKVSVNDFIIRAAAVTLKQMPGVNVTW
  DGEGPKQLPSVDISVAVATDKGLITPIIKDAAAKGIQEIADSVKVLSKKARDGKLMPEEYQGGSFSISNL
  GMFGIDEFAAVINPPQACILAVGRFRPVLKLTEDEEGNPQLQQHQLITVTMSSDSRVVDDELATRFLETF
  KANLENPMRLG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175094
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175094.5, NP_780303.1
• RefSeq Size: 2527
• RefSeq ORF: 1506
• Locus ID: 27402