Shmt2 (NM_028230) Mouse Tagged ORF Clone

CAT#: MG208109

Shmt2 (GFP-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Shmt2
• Synonyms: 2700043D08Rik; AA408223; AA986903; SHMT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208109 representing NM_028230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTATCCTTCTCTTTGCTTCGGACCACTCGGCCTCTGCAGAGATGTGGCCAGCTGGTCTGCATGGCTG
  CCCGGGCCCAGCACAGCAAGGTGGCCCAGACGCAGGCTGGGGAAGCAACTGGAGGTTGGACGGGCCAGGA
  GAGTTTATCAGACAGTGACCCTGAGATGTGGGAGCTTCTGCAGAGGGAGAAGGACAGACAGTGTCGCGGC
  CTGGAGCTCATCGCCTCAGAGAACTTCTGCAGCCGAGCTGCGCTGGAGGCCCTGGGGTCCTGTCTCAACA
  ACAAGTACTCGGAGGGTTACCCTGGCAAGAGATACTACGGAGGAGCGGAAGTGGTGGACGAGATCGAGCT
  GCTCTGCCAGCGCCGGGCCTTGGAAGCCTTTGACCTGGATCCGGCACAGTGGGGAGTCAATGTGCAGCCA
  TACTCAGGGTCCCCAGCCAATCTGGCTGCCTATACGGCCCTTCTGCAGCCTCATGATCGAATCATGGGGT
  TGGACCTGCCCGATGGGGGCCATCTCACCCATGGCTACATGTCTGATGTCAAGCGGATCTCCGCCACATC
  CATTTTCTTCGAGTCTATGCCCTATAAGCTCAATCCCCAAACTGGCCTCATCGACTACGACCAGCTGGCG
  CTGACCGCTCGGCTTTTCCGACCGCGGCTCATCATAGCTGGCACGAGTGCCTATGCCCGCCTCATTGACT
  ATGCACGCATGAAAGAGGTCTGTGATGAGGTCAGGGCACACCTGCTGGCAGACATGGCCCACATCAGTGG
  CCTGGTGGCTGCCAAGGTGATCCCCTCCCCTTTCAAGTACGCGGATGTTGTTACCACCACCACTCACAAG
  ACACTGCGAGGGGCCAGGTCAGGGCTCATCTTCTACCGGAAGGGAGTACGAACCGTAGACCCCAAGACTG
  GCAAAGAGATCCCTTATACCTTTGAGGACCGAATCAACTTCGCTGTGTTCCCATCCCTACAGGGTGGCCC
  CCACAACCACGCCATTGCTGCAGTAGCCGTGGCTCTCAAGCAGGCCTGCACCCCTATGTTCCGCGAGTAC
  TCCTTACAAGTGCTGAGGAACGCCCAGGCCATGGCTGATGCCCTGCTCAAGCGAGGATACTCGCTGGTGT
  CTGGTGGCACTGACACCCACCTGGTGCTGGTGGACCTGCGGCCCAAGGGCCTGGATGGAGCCCGAGCCGA
  ACGCGTGTTGGAACTTGTCTCCATCACAGCCAACAAGAACACCTGTCCTGGAGACCGGAGCGCCATTACT
  CCGGGGGGCTTGAGGCTTGGGGCCCCCGCGTTGACCTCTCGCCAGTTCCGTGAGGACGACTTCCGTAGAG
  TCGTCGATTTTATCGATGAGGGAGTCAACATTGGCTTGGAGGTGAAGCGCAAGACTGCCAAGCTCCAGGA
  TTTCAAATCCTTCCTGCTCAAAGACCCAGAGACAAGCCAGCGTTTGGCCAACCTCCGGCAACAGGTGGAA
  CAGTTTGCCAGGGGCTTCCCGATGCCTGGATTTGATGAACGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208109 representing NM_028230
  Red=Cloning site Green=Tags(s)
  
  MVSFSLLRTTRPLQRCGQLVCMAARAQHSKVAQTQAGEATGGWTGQESLSDSDPEMWELLQREKDRQCRG
  LELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQP
  YSGSPANLAAYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPQTGLIDYDQLA
  LTARLFRPRLIIAGTSAYARLIDYARMKEVCDEVRAHLLADMAHISGLVAAKVIPSPFKYADVVTTTTHK
  TLRGARSGLIFYRKGVRTVDPKTGKEIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREY
  SLQVLRNAQAMADALLKRGYSLVSGGTDTHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAIT
  PGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKRKTAKLQDFKSFLLKDPETSQRLANLRQQVE
  QFARGFPMPGFDER
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028230
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028230.3, NP_082506.1
• RefSeq Size: 2280
• RefSeq ORF: 1515
• Locus ID: 108037