Lipc (NM_008280) Mouse Tagged ORF Clone

CAT#: MG208201

Lipc (GFP-tagged) - Mouse lipase, hepatic (Lipc)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lipc
• Synonyms: AI256194; HL; Hpl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208201 representing NM_008280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATCCCCTCCAAATCTCCATTTTCCTGGTGTTCTGCATCTTTATCCAATCAAGTGCTTGTGGAC
  AAGGCGTGGGAACAGAGCCCTTTGGGAGAAGCCTTGGAGCTACTGAAGCTAGCAAGCCATTAAAGAAGCC
  AGAGACCAGATTCCTGCTCTTCCAAGATGAAAACGATCGCCTGGGCTGTCGTCTCAGACCTCAGCACCCG
  GAAACACTGCAGGAGTGTGGCTTCAACAGCTCTCAGCCGCTTATCATGATCATCCACGGGTGGTCGGTGG
  ATGGCTTGCTAGAAAACTGGATCTGGAAGATAGTGAGTGCGCTGAAGTCCCGACAGTCCCAACCTGTGAA
  TGTGGGGTTAGTGGACTGGATCTCCCTGGCATACCAGCACTACACCATTGCTGTTCAAAACACCCGTATT
  GTGGGCCAGGACGTGGCTGCTCTTCTCCTATGGCTGGAGGAATCTGCGAAGTTTTCTCGGAGCAAAGTTC
  ACCTAATTGGGTACAGCCTGGGAGCGCACGTCTCAGGGTTCGCAGGCAGCTCCATGGACGGGAAGAACAA
  GATTGGAAGAATCACAGGGCTGGACCCTGCGGGCCCTATGTTTGAGGGAACGTCCCCCAACGAGCGCCTT
  TCTCCTGATGATGCCAATTTTGTGGACGCCATTCATACCTTTACCAGGGAGCACATGGGCTTGAGTGTGG
  GCATCAAGCAGCCCATTGCCCACTATGACTTCTACCCCAATGGGGGCTCCTTCCAGCCTGGCTGCCACTT
  CCTGGAACTCTACAAACACATTGCAGAGCATGGCTTAAACGCCATAACCCAGACCATCAAATGTGCCCAT
  GAGCGCTCCGTGCACCTCTTCATTGACTCCTTGCAACACAGTGACCTGCAGAGCATCGGCTTCCAGTGCA
  GCGACATGGGCAGCTTCAGCCAAGGTCTATGCCTGAGCTGCAAGAAGGGCCGTTGCAACACTCTGGGTTA
  TGACATCCGCAAGGACCGGTCAGGCAAGAGCAAGAGGCTCTTCCTCATCACGCGAGCCCAGTCTCCCTTC
  AAAGTTTATCATTACCAGTTCAAGATCCAGTTCATCAATCAAATTGAGAAGCCGGTAGAGCCTACTTTTA
  CCATGTCGCTGCTGGGAACAAAAGAAGAAATAAAGAGAATTCCCATCACCCTGGGCGAAGGAATTACCAG
  CAATAAAACCTATTCCTTCCTTATCACACTGGACAAAGACATCGGCGAGTTGATCCTGCTCAAGTTCAAG
  TGGGAAAACAGTGCAGTGTGGGCCAATGTGTGGAACACAGTGCAGACCATCATGCTATGGGGCATAGAAC
  CTCACCACTCTGGCCTCATTCTGAAGACCATCTGGGTCAAAGCTGGAGAGACGCAGCAAAGAATGACATT
  TTGCCCCGAAAATCTGGATGACCTCCAGCTTCACCCGAGCCAGGAGAAAGTCTTTGTGAACTGTGAAGTG
  AAGTCAAAAAGACTGACTGAATCGAAAGAGCAGATGAGTCAAGAGACCCATGCAAAAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208201 representing NM_008280
  Red=Cloning site Green=Tags(s)
  
  MGNPLQISIFLVFCIFIQSSACGQGVGTEPFGRSLGATEASKPLKKPETRFLLFQDENDRLGCRLRPQHP
  ETLQECGFNSSQPLIMIIHGWSVDGLLENWIWKIVSALKSRQSQPVNVGLVDWISLAYQHYTIAVQNTRI
  VGQDVAALLLWLEESAKFSRSKVHLIGYSLGAHVSGFAGSSMDGKNKIGRITGLDPAGPMFEGTSPNERL
  SPDDANFVDAIHTFTREHMGLSVGIKQPIAHYDFYPNGGSFQPGCHFLELYKHIAEHGLNAITQTIKCAH
  ERSVHLFIDSLQHSDLQSIGFQCSDMGSFSQGLCLSCKKGRCNTLGYDIRKDRSGKSKRLFLITRAQSPF
  KVYHYQFKIQFINQIEKPVEPTFTMSLLGTKEEIKRIPITLGEGITSNKTYSFLITLDKDIGELILLKFK
  WENSAVWANVWNTVQTIMLWGIEPHHSGLILKTIWVKAGETQQRMTFCPENLDDLQLHPSQEKVFVNCEV
  KSKRLTESKEQMSQETHAKK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008280
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008280.2, NP_032306.2
• RefSeq Size: 1934
• RefSeq ORF: 1533
• Locus ID: 15450
• Gene Summary: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. [UniProtKB/Swiss-Prot Function]