Cdc25a (NM_007658) Mouse Tagged ORF Clone

CAT#: MG208257

Cdc25a (GFP-tagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdc25a
• Synonyms: D9Ertd393e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208257 representing NM_007658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGGCCCGGAGCCCCCCCACCGCCGCCGCCTGTTCTTCGCTTGCAGCCCCACGCCTGCGCCGC
  AGCCCACGGGGAAGATGCTGTTTGGCGCGTCAGCTGCTGGCGGACTGTCCCCTGTCACCAACCTGACGGT
  CACCATGGACCAGCTGGAAGGACTGGGCAGTGACTGTGAGAAAATGGAAGTGAGAAATAACAGCAGTCTA
  CAGAGAATGGGCTCCTCAGAATCGACCGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCCTTGGACAGTA
  AAGAAAACCTTGAAATTTCCCTGACGAGAATAAATTCCCTACCTCAGAAGCTCCTGGGATGTAGCCCAGC
  CCTGAAGAGGAGCCACTCTGATTCTCTAGACCACGACACCTTTCACCTCATCGACCAGGATGAAAATAAA
  GAAAATGAAGCATTTGAATTTAAAAAGCCAATACGACCTGCATCTCGTCACATCTACGAGGAGAGTAAAG
  ATCCCTTTACACACAGGCAGAATTCAGCCCCAGCTCGGATGCTATCTTCAAATGAAAGTGAATCAGGAAA
  TTTCAGTCCTCTTTTTATACCCCAGTCACCTGTAAAAGCCACTTTGTCCGATGAGGATGATGGCTTCATA
  GACCTTCTGGATGGCGAGAATATGAAGAATGATGAGGAGACCCCATCATGCATGGCAAGCCTCTGGACAG
  CTCCCCTTGTCATGAGGAGACCTGCAAACCTTGCCGATCGTTGCGGGCTGTTTGACTCCCCTTCCCCGTG
  CGGCTCCAGCACTCGGGCGGTGTTGAAGAGAGCAGACCGGTCTCACGAGGAGCCTCCTCGGGGTACAAAG
  AGGAGGAAGAGTGTGCCCAGCCCTGTGAAGGCGAAGGCGGATGTTCCGGAGCCCGCCCAGCTTCCATCCC
  AGTCTCTATCCCTGATGTCTTCCCCCAAAGGAACCATTGAGAACATTTTGGACAGTGACCCAAGAGACCT
  TATAGGAGATTTCTCCAAGGGTTATCTCTTTAATACAGTCTCTGGGAAGCATCAGGATTTGAAATATATT
  TCTCCAGAAATTATGGCATCTGTTTTGAATGGCAAGTTTGCCAGTCTCATTAAAGAGTTTGTTATCATTG
  ACTGCCGATACCCATATGAGTATGAAGGAGGTCACATCAAGGGTGCCGTGAATCTGCACATGGAAGAAGA
  GGTTGAAGACTTCTTACTTAAGAACCCTATTGTGCCTACTGATGGCAAGCGTGTCATTGTCGTGTTCCAC
  TGTGAGTTTTCCTCTGAAAGAGGCCCTCGAATGTGCCGATATGTGAGAGAGAGAGATAGGCTCGGCAATG
  AGTACCCCAAGCTCCACTACCCTGAGCTGTATGTCCTGAAGGGGGGATACAAGGAGTTCTTTTTGAAGTG
  CCAGTCTCACTGTGAACCCCCCAGCTACCGGCCAATGCACCATGAAGACTTTAAAGAAGACCTAAAGAAG
  TTCCGCACCAAGAGCCGGACCTGGGCAGGAGAAAAGAGCAAAAGAGAGATGTACAGTCGCCTGAAGAAGC
  TC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208257 representing NM_007658
  Red=Cloning site Green=Tags(s)
  
  MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSL
  QRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPQKLLGCSPALKRSHSDSLDHDTFHLIDQDENK
  ENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFI
  DLLDGENMKNDEETPSCMASLWTAPLVMRRPANLADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTK
  RRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYI
  SPEIMASVLNGKFASLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFH
  CEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKK
  FRTKSRTWAGEKSKREMYSRLKKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007658
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007658.3, NP_031684.3
• RefSeq Size: 3643
• RefSeq ORF: 1545
• Locus ID: 12530