Btd (NM_025295) Mouse Tagged ORF Clone

CAT#: MG208483

Btd (GFP-tagged) - Mouse biotinidase (Btd)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Btd
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208483 representing NM_025295
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAAGCAGATTTATGGTCTGCATTATGTCTGGAGCCCGCACTGCGCCTGCTCTCTTCTTCCTCGGCT
  GTTCTGCGCTTGCGTTGGGCGTCAGCTCGGCCTCCCAGGAGCACCGCGAGGCTGAGTACTACGTGGCTGC
  TGTGTACGAGCATCCATCGGTCCTGAGCCCAAACCCCCTGGAGCTCGTTAGCCGCCAGGAGGCCCTGGAG
  CTCATGAAGCAGAACCTCGACGTCTATGAACAGCAAGTGATGGCTGCAGCCCAGAAGGGTGTGCAGATTA
  TAGTGTTCCCAGAAGATGGTATCCACGGATTCAACTTTACAAGAACATCCATTTACCCGTTCTTGGATTT
  TATGCCGTCTCCCAAGCTGGTCAGGTGGAACCCGTGTCTGGAGCCCTTTCGGTTCAATGACACGGAGGTC
  CTCCAGCGCCTGAGTTGTATGGCCATCAAGGGAGGGATGTTCTTGGTGGCCAATCTTGGAACGAAACAGC
  CTTGCCTCAGCAGCGACCCTGGTTGCCCACAGGACGGGAGATACCAGTTTAACACAAATGTGGTGTTCAG
  CGACAATGGAACTCTTGTCGACCGCTACCGTAAGCACAACCTGTACTTTGAGGCGGCCTTTGATACCCCT
  GCTAACGTGGACCTCATCACCTTTGATACGCCCTTTGCTGGCAAGTTTGGCGTGTTCACTTGCTTTGACA
  TCCTATTCTTTGACCCTGCCGTCCGACTCCTCAGAGACTTTGAGGTGAAACACATTGTGTACCCCACAGC
  CTGGATGAACCAGCTACCGCTCTTGGCAGCCATTGAAATTCAGAAAGCATTCGCCACCGCCTTCGGTGTC
  AACGTTTTGGCAGCTAACATCCACCACCCGACTCTGGGGATGACCGGCAGTGGCATACACACCCCTCTCA
  AGTCCTTTTGGTACCATGACATGGACGACCCCAAAGGTCACCTTATAATTGCCCAGGTAGCCACAAACCC
  ACAGGGCCTCACTGGGACAGGGAATACAACTAGTGAGATGGACCCGTCCCATAGGAAGTTCTTAAAAATC
  CTGTCCGGAGATCCGTACTGTGAGAAGGATGCCCAGGAAGTGCACTGTGATGAGGCCGCCAAGTGGAACG
  TGAATGTCCCGCCCACCTTCCACTCGGAAATGATGTATGACAATTTCACCCTGGTCCCTGTCTGGGGAAC
  GGAAGGCCACCTGCAGGTGTGCTCCAACAGCCTCTGTTGCCACTTACTGTATGAGAGGCCCACCCTGTCC
  AAAGAGCTATATGCCCTGGGAGTCTTTGACGGACTCCACACCGTGCACGGCACCTACTACATTCAAACCT
  GTGCCCTGGTTAAGTGCGGGGGCCTTGGCTTCGACACCTGCGGGCAGGAGATCACGGAGGCCGAGGGCTT
  GTTTGACTTTCATCTGTGGGGGAACTTCAGCACTTTGTATATCTTTCCTTTATTTCTCACCTCGGGGATG
  ACTCTGGACACCCCTGACCAGCTTGGCTGGGAGAATGACCACTATTTCTTGAGGAAGAGGGGACTGTCCT
  CCGGCCTGGTGACAGCAGCTCTCTATGGACGGTTGTATGAGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208483 representing NM_025295
  Red=Cloning site Green=Tags(s)
  
  MRSRFMVCIMSGARTAPALFFLGCSALALGVSSASQEHREAEYYVAAVYEHPSVLSPNPLELVSRQEALE
  LMKQNLDVYEQQVMAAAQKGVQIIVFPEDGIHGFNFTRTSIYPFLDFMPSPKLVRWNPCLEPFRFNDTEV
  LQRLSCMAIKGGMFLVANLGTKQPCLSSDPGCPQDGRYQFNTNVVFSDNGTLVDRYRKHNLYFEAAFDTP
  ANVDLITFDTPFAGKFGVFTCFDILFFDPAVRLLRDFEVKHIVYPTAWMNQLPLLAAIEIQKAFATAFGV
  NVLAANIHHPTLGMTGSGIHTPLKSFWYHDMDDPKGHLIIAQVATNPQGLTGTGNTTSEMDPSHRKFLKI
  LSGDPYCEKDAQEVHCDEAAKWNVNVPPTFHSEMMYDNFTLVPVWGTEGHLQVCSNSLCCHLLYERPTLS
  KELYALGVFDGLHTVHGTYYIQTCALVKCGGLGFDTCGQEITEAEGLFDFHLWGNFSTLYIFPLFLTSGM
  TLDTPDQLGWENDHYFLRKRGLSSGLVTAALYGRLYERK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025295
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025295.1, NP_079571.1
• RefSeq Size: 1921
• RefSeq ORF: 1590
• Locus ID: 26363