Cyp27a1 (NM_024264) Mouse Tagged ORF Clone

CAT#: MG208548

Cyp27a1 (GFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyp27a1
• Synonyms: 1300013A03Rik; Cyp27
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208548 representing NM_024264
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGTGGAGCCGCACGAGGCTGAGATGGACGCTCCTGGACCCGCGTGTGGTGGGCCGTGGCCTCT
  GCCCACAAGGGGCCAGAGCCAAGGCCACGATCCCTGCAGCCCTCCAGGCACAGGAGAGTACGGAGGGTCC
  AGGAACAGGTCAAGACCGGCCGCGCCTGCGGAGTCCGGCGGAGCTTCCGGGGACCGGAACGCTACAATTT
  TTATTCCAGCTATTTCTACAAGGCTATGTGCTGCACTTGCCCGACCTCCAGGTGCTGAACAAGACCAAGT
  ATGGTCCAATGTGGACAACCTCCTTTGGGACTTACACCAATGTGAATCTGGCTAGTGCCCCACTCTTGGA
  GCAAGTGATGAGACAGGAGGGCAAGTACCCAATAAGAGACCACATGGATCAGTGGAAGGACCACCGAGAC
  CACAAGGGCCTCACCTATGGGATCTTCATCGCACAAGGAGAGCAATGGTACCATCTGCGTCAGGCTTTGA
  AACAGAGGCTGCTGAAGCCTGACGAGGCCGCGCTCTACACGGATGCCTTAAACGAGGTTATCAGTGACTT
  TATCACCCGGCTGGACCAGGTGCGGGCAGAGAGTGAATCAGGGGACCAGGTGCCAGACATGGCTCATCTT
  CTCTACCACCTTGCCTTGGAAGCCATCACCTATATCCTGTTTGAGAAAAGGATTGGCTGCCTGAAACCCT
  CCATTCCTGAGGACACTGCCGCCTTCATCAGATCTGTTGCAATCATGTTCCAGAACTCAGTCTATATCAC
  TTTCCTTCCCAAATGGACGCGTCCTCTGCTGCCCTTTTGGAAGCGATACCTGAATGGCTGGGATAACATT
  TTCTCCTTTGGAAAGAAGCTGATTGATGAAAAAGTCCAGGAGCTAAAAGCCCAGCTACAGGAAACTGGGC
  CAGATGGAGTCCGGGTATCTGGCTACCTGCACTTCCTGCTGACCAATGAATTGCTCAGTACTCAGGAGAC
  CATCGGCACCTTTCCTGAGCTGCTTTTGGCTGGGGTGGACACGACATCCAACACACTGACATGGGCCCTG
  TACCACCTTTCAAAGAGCCCAGAGATCCAGGAGGCCTTGCACAAGGAAGTGACTGGTGTGGTGCCCTTCG
  GGAAGGTGCCCCAGCACAAGGACTTTGCCCACATGCCTCTGCTAAAAGCTGTGATTAAGGAGACCCTGCG
  CCTCTACCCTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTTCTC
  TTCCCCAAGAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGGGATCCCAGTGTCTTTCCTGAGC
  CCAACAGCTTCCAGCCTCACCGATGGCTGAGGAAGAAAGAGGCTGATAACCCTGGGATCCTACATCCATT
  CGGCTCTGTGCCCTTCGGCTATGGGGTTCGGTCTTGCCTGGGTCGGAGGATTGCAGAACTGGAGATGCAA
  CTGATGCTGTCAAGGCTGGTACAGAAGTATGAGATTGCCCTGGCTCCCGGGATGGGAGAAGTAAAGACTG
  TGTCCCGCATCGTCCTGGTTCCCAGCAAGAAGGTGAGGCTGCATTTTCTGCAGAGACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208548 representing NM_024264
  Red=Cloning site Green=Tags(s)
  
  MAAWSRTRLRWTLLDPRVVGRGLCPQGARAKATIPAALQAQESTEGPGTGQDRPRLRSPAELPGTGTLQF
  LFQLFLQGYVLHLPDLQVLNKTKYGPMWTTSFGTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRD
  HKGLTYGIFIAQGEQWYHLRQALKQRLLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHL
  LYHLALEAITYILFEKRIGCLKPSIPEDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNI
  FSFGKKLIDEKVQELKAQLQETGPDGVRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDTTSNTLTWAL
  YHLSKSPEIQEALHKEVTGVVPFGKVPQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFL
  FPKNTQFVLCHYVVSRDPSVFPEPNSFQPHRWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQ
  LMLSRLVQKYEIALAPGMGEVKTVSRIVLVPSKKVRLHFLQRQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024264
• ORF Size: 1599 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024264.3, NP_077226.2
• RefSeq Size: 1891
• RefSeq ORF: 1602
• Locus ID: 104086