Ugt8a (NM_011674) Mouse Tagged ORF Clone

CAT#: MG208632

Ugt8a (GFP-tagged) - Mouse UDP galactosyltransferase 8A (Ugt8a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ugt8a
• Synonyms: AI850488; AW455908; Cgt; mCerGT; Ugt8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208632 representing NM_011674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCTTACACTCCATATTTCATGCTCCTGTGGAGTGCTGTTGGAATAGCAAGGGCTGCTAAAATCA
  TCATTGTGCCGCCAATTATGTTTGAAAGCCATTTGTACATTTTCAAGACACTAGCATCGGCCTTGCACGA
  GAGAGGCCATCACACCGTGCTCCTCCTCTCAGAAGGCAGAGACATTGCCCCATCTAATCACTACAGCCTC
  CAGCGGTACCCAGGCATCTTCAACAGTACCACCTCAGATGCTTTCCTGCAGTCTAAAATGAGGAATATTT
  TTTCTGGGAGATTGACAGCAGTTGAACTGGTTGACATACTGGATCACTATACTAAGAATTGTGACATGAT
  GGTTGGCAACCAAGCCCTAATCCAGGGTTTAAAAAAAGAAAAGTTTGACCTGCTACTGGTTGACCCCAAT
  GATATGTGTGGATTTGTGATCGCCCATCTTTTAGGCGTTAAGTATGCTGTATTTTCTACTGGCCTTTGGT
  ATCCTGCTGAAGTAGGAGCACCTGCTCCTTTAGCTTATGTCCCAGAGTTTAACTCACTCCTCACAGACCG
  CATGAACTTCCTGGAAAGGATGAAAAATACTGGTGTTTACCTCATATCCAGAATAGGGGTTAGCTTTCTG
  GTTCTCCCGAAATATGAGAGGATAATGCAGAAGTACAACCTGCTGCCTGCAAAGTCCATGTATGATTTGG
  TTCATGGGTCCAGCCTATGGATGTTATGTACTGACGTAGCACTGGAGTTTCCAAGACCAACGCTGCCTAA
  TGTTGTGTATGTTGGAGGAATCCTGACAAAGCCGGCCAGCCCACTGCCAGAAGATCTGCAGAGGTGGGTA
  AGTGGTGCTCAGGAACATGGCTTTGTCCTGGTGTCTTTTGGAGCTGGTGTCAAGTATCTGTCAGAAGACA
  TTGCCAACAAACTGGCAGGAGCTCTGGGGAGATTGCCTCAAAAAGTGATTTGGAGATTTTCTGGAACCAA
  ACCAAAGAACCTAGGAAACAACACTAAGCTCATAGAATGGCTGCCTCAAAATGACCTGCTTGGCCATTCA
  AATATCAGAGCCTTCCTAAGCCATGGTGGTTTGAACAGTATATTTGAAACTATGTATCATGGTGTCCCTG
  TAGTGGGAATCCCACTGTTTGGAGACCATTATGATACTATGACTAGAGTACAGGCAAAAGGCATGGGGAT
  CTTGTTAGAATGGAATACAGTTACTGAAGGAGAGCTGTATGATGCCCTGGTGAAAGTTATCAATAATCCC
  AGTTATCGGCAGAGGGCTCAGAAGTTATCGGAAATTCACAAGGATCAACCCGGCCACCCTGTCAATCGGA
  CTACCTATTGGATAGACTACATTCTTCGCCATGACGGAGCCCGTCACCTCCGTTCTGCTGTCCATCAGAT
  CTCTTTCTGCCAGTATTTCCTACTGGATATTGCCTTTGTGCTTCTGCTTGGTGCTGTCTTGCTCTACTTC
  ATATTGTCCTATGTGACAAAATTTATCTATAGAAAAATCAAAAGTCTATGGTCTAAAAATGAGCATAGCA
  CAGTTAATGGACATTACCAAAATGGAATCCGCAATGGCAAATACAAAGGCAATGGTCGTGTTAAACACGA
  AAAGAAGGTGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208632 representing NM_011674
  Red=Cloning site Green=Tags(s)
  
  MKSYTPYFMLLWSAVGIARAAKIIIVPPIMFESHLYIFKTLASALHERGHHTVLLLSEGRDIAPSNHYSL
  QRYPGIFNSTTSDAFLQSKMRNIFSGRLTAVELVDILDHYTKNCDMMVGNQALIQGLKKEKFDLLLVDPN
  DMCGFVIAHLLGVKYAVFSTGLWYPAEVGAPAPLAYVPEFNSLLTDRMNFLERMKNTGVYLISRIGVSFL
  VLPKYERIMQKYNLLPAKSMYDLVHGSSLWMLCTDVALEFPRPTLPNVVYVGGILTKPASPLPEDLQRWV
  SGAQEHGFVLVSFGAGVKYLSEDIANKLAGALGRLPQKVIWRFSGTKPKNLGNNTKLIEWLPQNDLLGHS
  NIRAFLSHGGLNSIFETMYHGVPVVGIPLFGDHYDTMTRVQAKGMGILLEWNTVTEGELYDALVKVINNP
  SYRQRAQKLSEIHKDQPGHPVNRTTYWIDYILRHDGARHLRSAVHQISFCQYFLLDIAFVLLLGAVLLYF
  ILSYVTKFIYRKIKSLWSKNEHSTVNGHYQNGIRNGKYKGNGRVKHEKKVR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011674
• ORF Size: 1623 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011674.4, NP_035804.2
• RefSeq Size: 3599
• RefSeq ORF: 1626
• Locus ID: 22239
• Gene Summary: Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. [UniProtKB/Swiss-Prot Function]