Slc1a3 (NM_148938) Mouse Tagged ORF Clone

CAT#: MG208665

Slc1a3 (GFP-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc1a3
• Synonyms: AI504299; B430115D02Rik; Eaat1; GLAST; GLAST-1; GLU-T; GluT-1; Gmt1; MGluT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208665 representing NM_148938
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAAAGCAACGGAGAAGAGCCTAGGATGGGGGGCAGGATGGAGAGATTGCAGCAAGGGGTCCGCA
  AGCGGACACTTCTGGCCAAGAAGAAAGTTCAGAGCCTCACCAAGGAAGATGTTAAGAGTTACCTGTTTCG
  GAATGCCTTCGTTCTGCTCACGGTCACTGCTGTCATTGTGGGTACAATCCTTGGATTTGCCCTCCGACCG
  TATAAAATGAGCTACCGGGAGGTGAAGTACTTTTCGTTCCCTGGGGAGCTTCTCATGAGGATGCTGCAGA
  TGCTGGTCTTGCCCCTGATCATCTCCAGTCTCGTCACAGGAATGGCGGCCCTAGATAGTAAGGCATCCGG
  GAAGATGGGGATGCGCGCTGTAGTCTATTACATGACTACTACCATCATTGCTGTGGTGATTGGCATAATC
  ATTGTCATCATCATCCACCCCGGAAAGGGCACAAAGGAAAACATGTACAGAGAAGGTAAAATCGTGCAGG
  TCACTGCAGCAGATGCCTTCCTGGATTTGATCAGGAACATGTTCCCTCCCAATCTGGTAGAAGCCTGCTT
  TAAACAGTTTAAAACCAGCTACGAGAAAAGAAGCTTTAAAGTGCCTATCCAGTCCAACGAAACACTTCTG
  GGCGCCGTGATCAACAACGTGTCAGAGGCCATGGAGACTCTGACCCGGATCCGGGAGGAGATGGTGCCCG
  TGCCTGGATCTGTGAATGGGGTCAATGCCCTGGGCCTAGTTGTCTTCTCCATGTGCTTCGGTTTCGTGAT
  CGGAAACATGAAGGAGCAGGGGCAAGCGCTGAGAGAGTTCTTTGATTCTCTTAACGAAGCCATCATGCGA
  TTGGTCGCGGTGATAATGTGGTATGCGCCTCTGGGCATCCTCTTCTTGATCGCAGGGAAGATTGTTGAGA
  TGGAAGACATGGGTGTGATTGGGGGACAGCTTGCCATGTACACCGTGACAGTCATTGTCGGCCTCCTCAT
  TCACGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCTGGGTTTTCATTGGA
  GGGTTGCTGCAAGCGCTCATCACAGCCCTTGGGACCTCCTCAAGTTCTGCCACCCTACCCATCACTTTCA
  AGTGCCTGGAAGAGAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTCCCCGTGGGGGCCACCAT
  TAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCTGCCATTTTCATCGCTCAAGTGAACAACTTTGAC
  CTGAACTTTGGACAGATTATAACAATAAGCATCACAGCCACGGCCGCAAGCATCGGGGCAGCCGGGATTC
  CTCAGGCCGGTCTGGTCACCATGGTCATCGTGCTGACATCTGTGGGCCTGCCCACAGATGACATCACACT
  CATCATTGCAGTGGACTGGTTTCTGGACCGCCTCCGAACCACCACCAACGTACTGGGTGACTCCCTCGGA
  GCAGGGATTGTCGAGCACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAATGGGGAACTCGGTGA
  TTGAGGAGAACGAAATGAAGAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCGGAGAAACCCGTGGC
  AGACAGCGAAACCAAGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208665 representing NM_148938
  Red=Cloning site Green=Tags(s)
  
  MTKSNGEEPRMGGRMERLQQGVRKRTLLAKKKVQSLTKEDVKSYLFRNAFVLLTVTAVIVGTILGFALRP
  YKMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGII
  IVIIIHPGKGTKENMYREGKIVQVTAADAFLDLIRNMFPPNLVEACFKQFKTSYEKRSFKVPIQSNETLL
  GAVINNVSEAMETLTRIREEMVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMR
  LVAVIMWYAPLGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIG
  GLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGATINMDGTALYEALAAIFIAQVNNFD
  LNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLG
  AGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNEPEKPVADSETKM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148938
• ORF Size: 4211 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_148938.2
• RefSeq Size: 4147
• RefSeq ORF: 1632
• Locus ID: 20512
• Gene Summary: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. [UniProtKB/Swiss-Prot Function]