Det1 (NM_029585) Mouse Tagged ORF Clone

CAT#: MG208737

Det1 (GFP-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Det1
• Synonyms: 2610034H20Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208737 representing NM_029585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCATCATGTTTCCACAATTAAACCTCGAAGAATCCAAAATCAAAATGTCATTCACCGCCTCGAAC
  GCCGGCGCATCAGTTCAGGCAAGGCGGGCACCCACTGGCATCAGGTCCGAGTGTTCCACCAGAACGTCTT
  CCCCAACTTCACGGTTGTCAACGTCGAAAAGCCTCCTTGTTTCTTACGGAAATTCTCGCCTGACGGGCGC
  TACTTTATTGCCTTTTCATCTGACCAGACATCTCTTGAAATCTATGAGTACCAGGGCTGTCAGGCAGCAG
  AGGACCTCTTGCAGGGATACGAAGGGGAGATCTTGTCCAATGGCAATGACCAGAGGTCGGTCAGCATCCG
  AGGTCGGCTCTTTGAGCGTTTTTTTGTTCTGCTGCACATTACCAACGTGGCGGCCAATGGCGAGCACTTG
  AACCGTGAGTGTAGCCTCTTCACAGATGACTGCCGCTGCGTCATCGTGGGCTCTGCTGCCTACCTCCCAG
  ACGAGCCTCACCCTCCATTCTATGAGGTGTATCGGAACAGTGAGTCGGTGACCCCCAATCCACGGTCCCC
  ACTTGAGGACTATTCCCTCCACATCATTGACCTTCACACTGGCCGTTTGTGTGACACACGCACATTCAAG
  TGTGACAAAGTGGTCTTGTCGCACAATCAAGGGCTCTACTTGTATAAAAACATCCTGGCCATATTGTCTG
  TGCAGCAGCAGACGATTCATGTCTTCCAGGTGACCCCTGAAGGCACCTTTATCGACGTTCGGACCATCGG
  CCGCTTCTGCTACGAGGATGACCTGCTCACTGTGTCTGCCGTTTTCCCTGAGGTGCAGCGGGACAGCCAG
  ACAGGCATGGCCAACCCTTTCAGGGACCCTTTCATCAACTCCTTGAAGCACCGCTTGCTGGTTTACCTCT
  GGCGCCGGGCAGAGCAGGATGGCAGTGCCATGGCGAAGAGGCGGTTCTTCCAGTACTTTGACCAGCTGCG
  CCAGCTGCGCATGTGGAAGATGCAGCTCCTGGACGAGAACCATCTGTTCATCAAGTACACCAGCGAGGAC
  GTAGTGACGCTGCGGGTCACAGACCCGTCTCAGGCCTCCTTCTTTGTGGTGTACAACATGGTGACAACTG
  AGGTGATTGCCGTGTTTGAGAACACATCCGATGAGCTTCTGGAACTCTTCGAGAACTTCTGTGACCTCTT
  CCGCAACGCCACCCTGCACAGTGAAGTCCAGTTCCCTTGCTCAGCTTCTAGCAACAACTTTGCAAGGCAG
  ATCCAGCGCCGGTTCAAAGACACGATTATAAATGCCAAGTATGGAGGGCACACGGAGGCTGTGCGCCGAC
  TGCTAGGCCAGCTCCCCATCAGTGCCCAGTCTTACAGTGGGAGCCCATACCTGGATTTGTCTCTCTTCAG
  CTACGATGACAAGTGGGTGTCAGTCATGGAGCGACCCAAGACTTGTGGAGATCACCCGATCAGGTTCTAT
  GCCCGGGACTCTGGCTTGCTGAAGTTTGAGATCCAGGCGGGCTTGCTGGGCCGCCCCATCAACCACACAG
  TGCGACGCCTCGTGGCCTTCACCTTTCACCCCTTTGAGCCATTTGCCATTTCTGTGCAGAGGACTAACGC
  AGAGTATGTTGTCAACTTCCACATGCGACACTGCTGCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208737 representing NM_029585
  Red=Cloning site Green=Tags(s)
  
  MDHHVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWHQVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGR
  YFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVSIRGRLFERFFVLLHITNVAANGEHL
  NRECSLFTDDCRCVIVGSAAYLPDEPHPPFYEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFK
  CDKVVLSHNQGLYLYKNILAILSVQQQTIHVFQVTPEGTFIDVRTIGRFCYEDDLLTVSAVFPEVQRDSQ
  TGMANPFRDPFINSLKHRLLVYLWRRAEQDGSAMAKRRFFQYFDQLRQLRMWKMQLLDENHLFIKYTSED
  VVTLRVTDPSQASFFVVYNMVTTEVIAVFENTSDELLELFENFCDLFRNATLHSEVQFPCSASSNNFARQ
  IQRRFKDTIINAKYGGHTEAVRRLLGQLPISAQSYSGSPYLDLSLFSYDDKWVSVMERPKTCGDHPIRFY
  ARDSGLLKFEIQAGLLGRPINHTVRRLVAFTFHPFEPFAISVQRTNAEYVVNFHMRHCCT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029585
• ORF Size: 1650 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_029585.3, NP_083861.1
• RefSeq Size: 2217
• RefSeq ORF: 1653
• Locus ID: 76375