Cdc45 (NM_009862) Mouse Tagged ORF Clone

CAT#: MG208941

Cdc45 (GFP-tagged) - Mouse cell division cycle 45 homolog (S. cerevisiae)-like (Cdc45l)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdc45
• Synonyms: Cdc45l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208941 representing NM_009862
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGTGACCGATTTCCGCAAGGAGTTCTACGAGACGGTCCACAACCAGAGGGTCCTTCTCTTTGTGG
  CCTCGGACGTGGATGCCTTGTGTGCTTGCAAGATCCTCCAGGCCCTGTTTCAGTGTGACCACGTCCAGTA
  TACGCTGGTTCCGGTTTCTGGGTGGCAAGAACTTGAAACTGCATATCTTGAACATAAAGAGCAGTTCTCC
  TATTTCATCCTCATAAACTGTGGAGCCAACGTGGACCTGTTGGATATCCTTCAGCCTGATGAAGACAGTA
  TATTTTTTGTGTGTGATACCCACAGGCCAGTGAACGTTGTGAATGTGTACAATGACACTCAGATCAAATT
  GCTTATTAAACAAGAGGATGACCTTGAGGTTCCTGCCTACGACGACATCTTTAGAGATGAAGCAGAGGAT
  GAAGATCTTTCAGACAGTGACGGTGATGGGTCAGAGCCTTCAGAGAAGCGCACACGGTTAGAAGAGGAGA
  TAGTGGAGCGAAACAGGAAGAGGAGGCAGCGCAGGGAGTGGGAGGCCCGAAGGAAAGACATCCTCTTTGA
  CTACGAGCAGTATGAATATTATGGGACATCGTCGGCCATGGTGATGTTTGACCTGGCGTGGATGATGTCC
  AAGGACCTGAATGACATGCTGTGGTGGGCCATTGTTGGACTGACAGACCAGTGGGTGCATGACAAGATCA
  CTCAAATGAAGTACGTGACTGATGTTGGCATCCTGCAGCGTCATGTGTCCCGTCATAACCACCGAAACGA
  GGCGGAGGAGAACATGCTCTCTGTAGACTGCACCCGGATCTCCTTCGAGTATGACCTCTGCCTGGTACTG
  TATCAGCACTGGTCCCTCCATGAAAGCCTGTACAACACCAGCTACACAGCAGCCAGGTTTAAGCTCTGGT
  CTGTGCATGGGCAGAAACGACTCCAGGAGTTCCTTGCAGACATGGGGCTTCCCCTGAAGCAAGTCAAGCA
  GAAATTTCAGTCCATGGACGTTTCCTTGAAGGGGAATCTGCGAGAAATGATTGAAGAATCTGCAAATAAA
  TTTGGGATGAAGGACATGCGTGTACAGACTTTCAGCATTCAGTTTGGGTTCAAGCATAAATTCCTGGCCA
  GTGATGTCGTCTTTGCCACCATGTCTCTAATGGAGAGTCCTGAGAAGGATGGCTCAGGGACAGACCACTT
  CATCCAGGCTCTCGATAGCCTCTCCAGGAGTAACCTGGACAAGCTATACCTTGGTCTAGAGCTCGCCAAG
  AAGCACCTCCAAGCCACACAACAGACCATCGCCAGCTGTCTCTGTACCAACCTCGTCACTTCCCAGGGCC
  CTTTTCTCTACTGCTCACTCATGGAGGGCACTCCAGATGTCACCCTGTTTTCCAAGCCAGCATCCTTGAG
  TCTGCTCAGCAGACATCTGCTCAAGTCCTTTGTGTACTCGACAAAGAATCGACGATGCAAGCTGCTGCCC
  CTGGTGATGGCTGCCCCGCTGAGCGTGGAACAGGGCACAGTGACCGTGGTGGGCATCCCCCCAGAGACTG
  ATAGCTCGGATAGAAAGAACTTTTTTGGTCGGGCTTTTGAGAAGGCAGCAGAAAGCACCAGCTCTCGGAC
  TCTACACAATTACTTTGACCTCTCAGTAATTGAGCTGAAGGCTGAGGACCGGAGCAAGTTCCTGGACGCT
  CTTGTGTCACTGCTGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208941 representing NM_009862
  Red=Cloning site Green=Tags(s)
  
  MFVTDFRKEFYETVHNQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAYLEHKEQFS
  YFILINCGANVDLLDILQPDEDSIFFVCDTHRPVNVVNVYNDTQIKLLIKQEDDLEVPAYDDIFRDEAED
  EDLSDSDGDGSEPSEKRTRLEEEIVERNRKRRQRREWEARRKDILFDYEQYEYYGTSSAMVMFDLAWMMS
  KDLNDMLWWAIVGLTDQWVHDKITQMKYVTDVGILQRHVSRHNHRNEAEENMLSVDCTRISFEYDLCLVL
  YQHWSLHESLYNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQSMDVSLKGNLREMIEESANK
  FGMKDMRVQTFSIQFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYLGLELAK
  KHLQATQQTIASCLCTNLVTSQGPFLYCSLMEGTPDVTLFSKPASLSLLSRHLLKSFVYSTKNRRCKLLP
  LVMAAPLSVEQGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRTLHNYFDLSVIELKAEDRSKFLDA
  LVSLLS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009862
• ORF Size: 1698 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009862.1, NP_033992.1
• RefSeq Size: 2161
• RefSeq ORF: 1701
• Locus ID: 12544
• Gene Summary: Required for initiation of chromosomal DNA replication. [UniProtKB/Swiss-Prot Function]