Me1 (NM_008615) Mouse Tagged ORF Clone

CAT#: MG209005

Me1 (GFP-tagged) - Mouse malic enzyme, supernatant (Mod1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Me1
• Synonyms: D9Ertd267e; Mdh-1; Mod-1; Mod1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209005 representing NM_008615
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCCGAGCCCCCCGCCGCCGACACACCCACCAGCGCGGCTACCTGCTGACGCGGGACCCGCATC
  TCAACAAGGACTTGGCTTTTACTCTGGAAGAGAGACAGCAATTGAACATTCATGGATTGTTGCCGCCCTG
  CATCGTCAGCCAGGAGCTCCAGGTCCTTAGAATAATTAAGAATTTCGAGCGACTGAACTCTGACTTCGAC
  AGGTATCTTCTGTTAATGGACCTGCAAGACAGAAATGAGAAGCTCTTCTACAGCGTGCTCATGTCTGATG
  TTGAAAAGTTCATGCCTATTGTTTACACCCCCACCGTGGGCCTTGCATGCCAGCAGTACAGTTTGGCATT
  CCGGAAGCCAAGAGGCCTCTTTATTAGTATCCATGACAAAGGGCACATTGCTTCAGTTCTTAATGCATGG
  CCAGAGGATGTCGTTAAGGCTATTGTGGTAACTGACGGAGAGCGCATCCTTGGCTTGGGAGACCTTGGCT
  GTAATGGGATGGGCATCCCTGTGGGTAAACTGGCCCTGTACACGGCATGTGGAGGGGTGAACCCACAACA
  GTGTCTACCCATCACTTTGGATGTGGGAACAGAAAATGAGGAGTTACTTAAGGATCCACTGTACATCGGG
  CTGCGGCACCGGCGAGTCAGAGGCCCTGAGTATGACGCCTTCCTGGATGAGTTCATGGAGGCAGCGTCTT
  CCAAATATGGCATGAATTGCCTTATTCAGTTTGAAGATTTTGCCAATCGGAATGCATTTCGTCTCCTGAA
  CAAGTATCGAAACAAGTATTGCACATTTAACGATGATATTCAAGCGTCTGTTGCGGTTGCGGGTCTCCTT
  GCAGCTCTTCGAATAACCAAGAACAAGCTCTCTGATCAGACAGTGCTGTTCCAGGGAGCTGGAGAGGCTG
  CCTTGGGGATTGCTCACTTGGTTGTTATGGCCATGGAGAAAGAAGGTTTATCAAAGGAGAATGCTAGAAA
  GAAGATATGGTTGGTTGACTCAAAAGGACTAATAGTTAAGGGTCGGGCTTCTCTCACAGAAGAGAAAGAG
  GTGTTTGCCCATGAACATGAAGAAATGAAGAATCTGGAAGCCATTGTTCAAAAGATAAAACCAACTGCCC
  TCATAGGAGTTGCTGCAATTGGTGGTGCTTTCACTGAACAAATTCTCAAGGATATGGCTGCCTTCAACGA
  GCGGCCCATCATCTTTGCTTTGAGCAATCCGACCAGCAAAGCGGAGTGCTCTGCAGAGCAGTGCTACAAG
  GTGACCAAGGGACGTGCAATCTTTGCCAGCGGCAGTCCTTTTGATCCAGTCACTCTCCCAGATGGACGGA
  CTCTGTTTCCTGGCCAAGGCAACAATTCCTACGTGTTCCCTGGAGTTGCTCTTGGGGTGGTGGCCTGCGG
  ACTGAGACACATCGATGATAAGGTCTTCCTCACCACTGCTGAGGTCATATCTCAGCAAGTGTCAGATAAA
  CACCTGCAAGAAGGCCGGCTCTATCCTCCTTTGAATACCATTCGAGACGTTTCGTTGAAAATTGCAGTAA
  AGATTGTGCAAGATGCATACAAAGAAAAGATGGCCACTGTTTATCCTGAACCCCAAAACAAAGAAGAATT
  TGTCTCCTCCCAGATGTACAGCACTAATTATGACCAGATCCTACCTGATTGTTATCCGTGGCCTGCAGAA
  GTCCAGAAAATACAGACCAAAGTCAACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209005 representing NM_008615
  Red=Cloning site Green=Tags(s)
  
  MEPRAPRRRHTHQRGYLLTRDPHLNKDLAFTLEERQQLNIHGLLPPCIVSQELQVLRIIKNFERLNSDFD
  RYLLLMDLQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAW
  PEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIG
  LRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQASVAVAGLL
  AALRITKNKLSDQTVLFQGAGEAALGIAHLVVMAMEKEGLSKENARKKIWLVDSKGLIVKGRASLTEEKE
  VFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYK
  VTKGRAIFASGSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTAEVISQQVSDK
  HLQEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYPWPAE
  VQKIQTKVNQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008615
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008615.1, NP_032641.1
• RefSeq Size: 3129
• RefSeq ORF: 1719
• Locus ID: 17436