Pigt (BC034175) Mouse Tagged ORF Clone

CAT#: MG209067

Pigt (GFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pigt
• Synonyms: CGI-06, NDAP, Ndap7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209067 representing BC034175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTCGGTTTGCCGTTGCGGTTACTGGTGCTGTTGCTGGTGGGACGGGGCTGCTGTGGCTGCGCGG
  AGGGCCCACGCGACAGCCTGCGAGAAGAGCTCGTTATCACTCCACTGCCTTCCGGCGACGTGGCCGCCAC
  ATTCCAGTTCCGCACGCGTTGGGATTCGGATCTGCAGCGGGAAGGAGTGTCCCATTACAGGCTCTTCCCT
  AAAGCCCTGGGACAGCTGATCTCCAAGTACTCCCTGCGGGAGCTCCACCTGTCGTTCACGCAAGGCTTTT
  GGAGGACGCGATACTGGGGGCCACCCTTCCTGCAGGCTCCGTCAGGTGCAGAGCTCTGGGTCTGGTTCCA
  AGACACTGTCACCGATGTGGATAAGTCCTGGAGGGAGCTCAGCAATGTCCTCTCGGGGATCTTCTGTGCA
  TCCCTCAACTTCATCGACGCCACCAATACCGTCACTCCCACCGCCTCCTTCAAACCTCTGGGACTGGCCA
  ATGACACTGACGACTACTTCCTGCGCTACGCTGTGCTGCCTCGGGAGGTTGTCTGCACTGAGAACCTCAC
  GCCATGGAAGAAGCTCTTGCCCTGCAGTTCCAAGGCCGGTCTCTCAGTGCTATTGAAAGCAGACCGCTTG
  TTCCACACCAGTTACCACTCCCAGGCAGTGCACATCCGGCCTATCTGCAGAAATGCTCACTGTACCAGCA
  TCTCCTGGGAGCTGAGGCAGACCCTTTCGGTTGTCTTTGATGCCTTCATCACAGGACAGGGGAAGAAAGA
  CTGGTCCCTCTTCCGCATGTTCTCCCGGACTCTCACAGAGGCCTGTCCATTGGCGTCTCAGAGCCTAGTT
  TATGTGGACATCACAGGCTACAGCCAGGATAATGAAACACTGGAGGTGAGCCCTCCCCCAACTTCCACAT
  ACCAGGATGTCATTTTGGGCACCAGGAAGACCTATGCCGTCTATGACTTGTTTGACACAGCCATGATCAA
  TAACTCTCGAAACCTCAACATCCAGCTCAAATGGAAGAGACCCCCAGATAATGAGGCCCTACCCGTGCCC
  TTCCTGCATGCGCAGCGGTACGTGAGTGGCTATGGACTGCAGAAGGGAGAGCTGAGTACTCTGCTGTACA
  ACTCCCATCCTTATCGGGCCTTCCCCGTGCTGCTGCTGGATGTCGTGCCCTGGTACCTGCGGCTGTATGT
  GCACACGCTCACCATTACCTCCAAGGGCAAGGAGAATAAACCAAGTTACATCCACTACCAACCTGCTCAG
  GACAGGCAGCAGCCCCACCTCCTGGAGATGCTCATTCAGCTGCCAGCCAACTCCGTCACCAAGGTCTCCA
  TCCAGTTTGAACGAGCCCTGCTCAAGTGGACAGAGTACACACCAGACCCCAACCACGGCTTCTATGTCAG
  CCCATCTGTCCTCAGCGCCCTTGTGCCCAGTGTGGTGGCAGCCAAACCAGTGGACTGGGAAGGGAGCCCT
  CTCTTCAACACCTTGTTCCCGGTGTCTGATGGCTCCAGCTACTTTGTCCGACTCTACACAGAGCCCTTGC
  TAGTGAACCTGCCCACCCCGGACTTCAGCATGCCCTACAATGTGATCTGCCTTACGTGCACGGTGGTGGC
  CGTGTGCTATGGCTCCTTCTACAATCTCCTCACCCGAACCTTTCACATCGAAGAGCCCAAGTCGGGCGGC
  CTGGCCAAGCGGCTGGCTAACCTCATCCGCCGTGCCCGTGGTGTGCCCCCTCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209067 representing BC034175
  Red=Cloning site Green=Tags(s)
  
  MPLGLPLRLLVLLLVGRGCCGCAEGPRDSLREELVITPLPSGDVAATFQFRTRWDSDLQREGVSHYRLFP
  KALGQLISKYSLRELHLSFTQGFWRTRYWGPPFLQAPSGAELWVWFQDTVTDVDKSWRELSNVLSGIFCA
  SLNFIDATNTVTPTASFKPLGLANDTDDYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLKADRL
  FHTSYHSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEACPLASQSLV
  YVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSRNLNIQLKWKRPPDNEALPVP
  FLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDVVPWYLRLYVHTLTITSKGKENKPSYIHYQPAQ
  DRQQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPVDWEGSP
  LFNTLFPVSDGSSYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEPKSGG
  LAKRLANLIRRARGVPPL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC034175
• ORF Size: 1734 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC034175.1
• RefSeq Size: 2322
• RefSeq ORF: 1736
• Locus ID: 78928