Chrna4 (NM_015730) Mouse Tagged ORF Clone

CAT#: MG209587

Chrna4 (GFP-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 4 (Chrna4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chrna4
• Synonyms: Acra-4; Acra4; EBN1; ENFL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209587 representing NM_015730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATCGGGGGCTCCGGGGCGCCGCCGCCGCTGCTGCTCCTGCCGCTCCTGCTGCTCTTAGGGACCG
  GCCTCTTGCCTGCTAGCAGCCACATAGAGACCCGGGCCCATGCGGAGGAGCGGCTCCTGAAGAGACTCTT
  CTCTGGCTACAACAAGTGGTCTCGGCCAGTAGCCAATATCTCAGATGTGGTCCTTGTCCGCTTTGGCTTG
  TCGATTGCTCAGCTCATTGATGTGGATGAGAAAAACCAGATGATGACGACCAACGTGTGGGTGAAGCAGG
  AGTGGCATGACTACAAACTGCGCTGGGACCCTGGTGACTACGAGAATGTCACCTCCATCCGCATCCCATC
  TGAACTCATCTGGAGGCCTGACATCGTCCTCTACAACAACGCGGACGGGGACTTTGCAGTCACCCACCTA
  ACCAAAGCCCACCTGTTCTATGATGGGCGTGTGCAGTGGACACCCCCGGCCATCTATAAGAGCTCCTGCA
  GCATCGACGTCACCTTCTTCCCCTTCGACCAGCAGAACTGTACCATGAAGTTTGGGTCCTGGACCTACGA
  CAAGGCCAAGATTGACTTGGTGAGCATGCACAGCCGTGTGGACCAACTGGACTTCTGGGAAAGTGGGGAG
  TGGGTCATTGTGGATGCCGTGGGCACCTACAACACCAGGAAGTATGAATGCTGTGCCGAGATCTATCCTG
  ACATCACCTACGCCTTCATCATCCGCCGACTGCCACTGTTCTACACCATCAACCTTATCATCCCGTGCCT
  GCTCATCTCCTGCCTCACCGTGCTGGTCTTCTATCTGCCCTCGGAGTGCGGCGAGAAGGTCACGCTGTGC
  ATCTCGGTGCTGCTTTCTCTCACCGTCTTCCTGCTGCTCATCACCGAGATCATCCCGTCCACCTCGCTGG
  TCATCCCGCTCATCGGCGAGTACCTGCTCTTCACCATGATCTTCGTCACCCTCTCCATTGTCATCACGGT
  CTTCGTGCTCAATGTACACCACCGCTCACCACGCACACACACCATGCCCGCCTGGGTGCGCAGAGTCTTC
  CTGGACATTGTGCCCCGTCTCCTCTTCATGAAGCGCCCATCTGTGGTCAAAGACAACTGCCGGAGACTTA
  TCGAATCCATGCACAAGATGGCCAACGCCCCTCGTTTCTGGCCAGAGCCTGAGAGTGAGCCCGGCATCTT
  GGGTGACATCTGCAACCAAGGCCTGTCACCTGCCCCAACTTTCTGCAACCGCATGGACACAGCAGTCGAG
  ACCCAGCCTACATGCAGGTCACCCTCCCACAAGGTCCCTGACTTGAAGACATCAGAGGTTGAGAAGGCCA
  GTCCCTGTCCATCACCTGGCTCTTGTCACCCACCCAATAGCAGTGGGGCCCCAGTGCTCATCAAAGCCAG
  GTCCCTGAGCGTCCAGCATGTGCCCAGCTCCCAGGAAGCAGCCGAGGGCAGCATCCGCTGCCGGTCTCGG
  AGTATCCAGTACTGTGTTTCCCAAGATGGAGCTGCTTCCCTGACTGAGAGCAAGCCCACTGGCTCCCCAG
  CCTCCCTGAAGACCCGTCCATCCCAGCTTCCAGTGTCAGACCAGACCTCTCCATGCAAATGCACATGCAA
  GGAACCATCTCCTGTGTCCCCCATCACTGTGCTCAAGGCTGGAGGCACCAAAGCACCTCCCCAACACCTG
  CCCCTGTCACCAGCCCTGACACGGGCAGTAGAAGGCGTCCAGTACATTGCAGACCACCTCAAGGCAGAAG
  ACACAGACTTCTCGGTGAAGGAGGACTGGAAATACGTGGCCATGGTCATTGACCGAATCTTCCTCTGGAT
  GTTCATCATTGTCTGCCTTCTGGGCACTGTGGGACTCTTCCTGCCTCCATGGTTGGCTGGTATGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209587 representing NM_015730
  Red=Cloning site Green=Tags(s)
  
  MEIGGSGAPPPLLLLPLLLLLGTGLLPASSHIETRAHAEERLLKRLFSGYNKWSRPVANISDVVLVRFGL
  SIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHL
  TKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSRVDQLDFWESGE
  WVIVDAVGTYNTRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLC
  ISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVF
  LDIVPRLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPEPESEPGILGDICNQGLSPAPTFCNRMDTAVE
  TQPTCRSPSHKVPDLKTSEVEKASPCPSPGSCHPPNSSGAPVLIKARSLSVQHVPSSQEAAEGSIRCRSR
  SIQYCVSQDGAASLTESKPTGSPASLKTRPSQLPVSDQTSPCKCTCKEPSPVSPITVLKAGGTKAPPQHL
  PLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015730
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015730.5, NP_056545.3
• RefSeq Size: 4509
• RefSeq ORF: 1890
• Locus ID: 11438
• Gene Summary: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions. [UniProtKB/Swiss-Prot Function]