Pck2 (BC010318) Mouse Tagged ORF Clone

CAT#: MG209685

Pck2 (GFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (cDNA clone MGC:11639 IMAGE:3596227)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pck2
• Synonyms: 1810010O14Rik|9130022B02Rik|PEPCK-M
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209685 representing BC010318
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTATGTACCTCCCCGGCCTGCGGCTTAGCCGGCACGGGCTAAGGCCCTGGTGCTGGTCACCGT
  GCCGTAGCATCCAAACCCTGCGTGTGCTCAGTGGAGATATGAGTCAGCTGCCGGCTGGAGTTCGAGACTT
  TGTGGCGCGCAGTGCGCATCTGTGCCAACCAGAGGGCATCCACATCTGTGATGGGACCGAGGCTGAGAAC
  ACTGCCATACTGGCCCTGCTGGAAGAACAGGGTCTTATCCGCAAACTCCCCAAGTATAAGAACTGCTGGC
  TGGCCCGCACAGACCCCAAGGATGTGGCACGGGTAGAAAGCAAGACGGTGATTGTAACTCCTTCGCAGCG
  GGACACAGTGCCTCTCCTGGCTGGTGGGGCCAGGGGGCAGCTGGGCAACTGGATGTCCCCAGATGAGTTC
  CAGAGAGCTGTGGACGAGAGATTCCCAGGATGCATGCAGGGCCGCATTATGTATGTGCTTCCATTCAGCA
  TGGGTCCCGTGGGCTCCCCACTCTCCCGCATTGGAGTGCAGCTCACTGACTCGGCTTACGTGGTGGCCAG
  TATGCGTATTATGACCCGCTTGGGGACACCTGTACTTCAGGCCCTGGGAGATGGTGACTTCATCAAGTGT
  CTGCATTCAGTGGGCCAGCCCCTGACTGGACATGGGGATCCTGTGGGCCAGTGGCCGTGCAATCCAGAAA
  AAACCCTGATTGGCCACGTGCCAGACCAGCGGGAGATCGTCTCCTTCGGCAGCGGCTATGGTGGTAACTC
  CTTGCTGGGCAAGAAGTGCTTTGCCCTGCGCATCGCCTCTCGCCTGGCCAGGGATGAGGGCTGGCTGGCA
  GAGCACATGCTGATTTTGGGCATCACCAACCCTGCAGGGAAAAAGCGCTACGTGGCAGCTGCTTTCCCCA
  GTGCCTGTGGCAAGACCAATCTGGCCATGATGAGACCTGCATTGCCGGGCTGGAAAGTGGAGTGTGTGGG
  GGATGACATCGCCTGGATGAGGTTTGACAGTGAAGGTCAACTCCGGGCCATCAACCCTGAGAATGGCTTC
  TTTGGGGTGGCCCCTGGTACCTCTGCTGCCACCAATCCCAATGCCATGGCCACAATTCAGAGTAATACTC
  TCTTCACCAACGTGGCTGAGACCAGTGATGGCGGTGTGTACTGGGAAGGCATTGACCAGCCTCTTCCGCC
  TGGTGTCACCATAACCTCGTGGCTGGGAAAGCCCTGGAAACCTGGTGACAAGGAACCCTGTGCACATCCA
  AACTCGCGCTTTTGTGTCCCAGCTCGCCAGTGCCCCATCATGGACCCAGCCTGGGAAGCACCAGAAGGTG
  TCCCTATTGATGCCATCATCTTTGGAGGCCGCCGACCCAAAGGGGTACCACTGGTGTACGAGGCCTTCAA
  CTGGCGTCATGGGGTGTTTGTAGGTAGCGCCATGCGCTCTGAGTCCACTGCCGCTGCGGAGCACAAAGGA
  AAGACCATTATGCATGATCCCTTTGCCATGCGGCCTTTTTTTGGCTATAACTTTGGACGCTACCTGGAAC
  ACTGGTTGAGCATGGAGGGACAAAAAGGTGCCCGGCTGCCTCGTATCTTCCATGTCAATTGGTTCCGGAG
  AGATGAAGCAGGCTGCTTCCTGTGGCCAGGCTTTGGAGAGAATGCTCGTGTGCTAGACTGGATCTGCCGA
  AGATTAGAAGGAGAAGACAGTGCCCAAGAGACTCCCATTGGGCTAGTACCAAAGGAAGGAGCCCTGGACC
  TCAGTGGCCTCAGCGCAGTGGACACCAGTCAGCTGTTCTCCATCCCCAAGGACTTCTGGGAGCAGGAGGT
  TCGTGATATTCGGGGCTACCTGACAGAGCAAGTCAACCAGGACCTGCCCAAGGAGGTGTTGGCTGAGCTC
  GAGGCTCTGGAAGGACGTGTACAAAAAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209685 representing BC010318
  Red=Cloning site Green=Tags(s)
  
  MAAMYLPGLRLSRHGLRPWCWSPCRSIQTLRVLSGDMSQLPAGVRDFVARSAHLCQPEGIHICDGTEAEN
  TAILALLEEQGLIRKLPKYKNCWLARTDPKDVARVESKTVIVTPSQRDTVPLLAGGARGQLGNWMSPDEF
  QRAVDERFPGCMQGRIMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFIKC
  LHSVGQPLTGHGDPVGQWPCNPEKTLIGHVPDQREIVSFGSGYGGNSLLGKKCFALRIASRLARDEGWLA
  EHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGQLRAINPENGF
  FGVAPGTSAATNPNAMATIQSNTLFTNVAETSDGGVYWEGIDQPLPPGVTITSWLGKPWKPGDKEPCAHP
  NSRFCVPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKG
  KTIMHDPFAMRPFFGYNFGRYLEHWLSMEGQKGARLPRIFHVNWFRRDEAGCFLWPGFGENARVLDWICR
  RLEGEDSAQETPIGLVPKEGALDLSGLSAVDTSQLFSIPKDFWEQEVRDIRGYLTEQVNQDLPKEVLAEL
  EALEGRVQKM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010318
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC010318.1
• RefSeq Size: 2143
• RefSeq ORF: 1922
• Locus ID: 74551