Man1a2 (NM_010763) Mouse Tagged ORF Clone

CAT#: MG209697

Man1a2 (GFP-tagged) - Mouse mannosidase, alpha, class 1A, member 2 (Man1a2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Man1a2
• Synonyms: AI428775; AI528764; AI854422; Man1b; PCR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209697 representing NM_010763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACCCCAGCGCTGCTGCCCCTCTCTGGTCGTAGGATACCACCTTTGAACCTGGGGCCGCCCTCCT
  TCCCACATCACAGGGCTACCTTGAGACTCTCCGAGAAGTTTATCCTTCTCCTCATCCTTAGTGCCTTCAT
  CACCCTGTGTTTTGGGGCATTCTTCTTCCTTCCAGACTCTTCAAAACACAAACGCTTTGATCTGGGCTTA
  GAAGATGTGTTAATTCCTCACGTAGATGCCGGCAAAGGAGCTAAAAACCCCGGCGTCTTCCTGATCCATG
  GACCCGACGAACACAGACACAGGGAAGAAGAAGAGCGTCTGAGAAATAAGATTAGAGCTGACCATGAGAA
  AGCCCTGGAAGAAGCAAAAGAAAAATTAAGAAAGTCAAGAGAGGAAATCCGTGCAGAAATTCAGACAGAG
  AAAAACAAAGTAGCCCAAGCAATGAAGACAAAAGAGACCAGGGTACTGCCGCCTGTCCCTGTCCCACAAC
  GTGTAGGGGTCAGTGGTGGGGATCCAGAAGACATGGAGATCAAGAAGAAAAGAGACAAAATTAAAGAGAT
  GATGAAACATGCCTGGGATAATTACAGAACATACGGATGGGGACATAATGAACTAAGGCCTATTGCAAGG
  AAAGGCCATTCCACTAACATATTCGGAAGCTCACAGATGGGTGCCACCATAGTGGATGCTTTGGATACCC
  TTTATATCATGGGGCTTCATGATGAATTCATGGATGGGCAAAGATGGATTGAAGAAAACCTTGATTTCAG
  TGTGAATTCAGAAGTGTCTGTCTTTGAAGTTAACATTCGCTTTATTGGAGGGCTCCTCGCTGCATATTAC
  CTGTCAGGAGAGGAAATATTCAAGACTAAAGCAGTGCAGTTGGCTGAGAAACTCCTTCCTGCCTTTAACA
  CACCTACTGGGATTCCCTGGGCAATGGTGAACCTGAAAAGTGGAGTAGGTCGAAACTGGGGCTGGGCGTC
  TGCAGGCAGCAGCATCCTGGCTGAGTTCGGCACCCTGCACATGGAGTTTGTGCACCTCAGCTACTTGACC
  GGTGACTTGACTTACTATAATAAGGTCATGCACATTCGGAAACTACTGCAGAAAATGGAACGCCCAAATG
  GTCTTTATCCAAATTATTTAAACCCAAGAACAGGGCGCTGGGGTCAGTATCACACATCAGTTGGTGGTCT
  GGGAGATAGTTTTTATGAATACTTACTGAAAGCATGGCTGATGTCAGATAAAACAGACCACGAGGCAAGA
  AGGATGTATGACGATGCTGTTGAGGCTATAGAAAAACATCTTATTAAGAAGTCCCGAGGAGGTCTGGTTT
  TTATTGGAGAATGGAAGAATGGACACTTGGAAAGGAAGATGGGGCACTTGGCCTGCTTTGCTGGGGGAAT
  GTTTGCCCTTGGAGCAGATGGTTCCAGAAAGGATAAAGCTGGCCACTACTTAGAACTAGGGGCAGAAATT
  GCACGAACATGTCATGAGTCATATGACAGAACTGCATTGAAACTAGGTCCGGAGTCATTCAAGTTTGATG
  GTGCAGTGGAAGCCGTGGCTGTGCGGCAGGCTGAAAAGTATTACATCCTTCGTCCAGAAGTAATTGAAAC
  CTATTGGTATCTATGGCGATTTACCCACGACCCAAGATACAGGCAGTGGGGCTGGGAAGCAGCACTGGCT
  ATTGAGAAGTCGTGCCGGGTCAGCGGTGGGTTTTCTGGTGTCAAGGATGTATACGCCCCGACCCCTGTGC
  ATGACGACGTGCAGCAGAGCTTTTTTCTTGCTGAAACATTAAAATACTTGTACCTGCTGTTCTCTGGCGA
  TGACCTTCTACCTTTAGACCACTGGGTGTTTAACACAGAGGCGCACCCTCTGCCGGTGTTGCGCTTAGCC
  AACAGCACTCTTTCAGGTAATCCTGCTGTCCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209697 representing NM_010763
  Red=Cloning site Green=Tags(s)
  
  MTTPALLPLSGRRIPPLNLGPPSFPHHRATLRLSEKFILLLILSAFITLCFGAFFFLPDSSKHKRFDLGL
  EDVLIPHVDAGKGAKNPGVFLIHGPDEHRHREEEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTE
  KNKVAQAMKTKETRVLPPVPVPQRVGVSGGDPEDMEIKKKRDKIKEMMKHAWDNYRTYGWGHNELRPIAR
  KGHSTNIFGSSQMGATIVDALDTLYIMGLHDEFMDGQRWIEENLDFSVNSEVSVFEVNIRFIGGLLAAYY
  LSGEEIFKTKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFVHLSYLT
  GDLTYYNKVMHIRKLLQKMERPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEAR
  RMYDDAVEAIEKHLIKKSRGGLVFIGEWKNGHLERKMGHLACFAGGMFALGADGSRKDKAGHYLELGAEI
  ARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALA
  IEKSCRVSGGFSGVKDVYAPTPVHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLRLA
  NSTLSGNPAVR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010763
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010763.2, NP_034893.1
• RefSeq Size: 7960
• RefSeq ORF: 1926
• Locus ID: 17156
• Gene Summary: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2). [UniProtKB/Swiss-Prot Function]