Tie1 (BC060182) Mouse Tagged ORF Clone

CAT#: MG209734

Tie1 (GFP-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tie1
• Synonyms: TIE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209734 representing BC060182
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGGTGGGGATCCTCTTTGCTGCTCCCCACTCTTTTCTTGGCCTCTCATGTTGGTGCGTCTGTTG
  ACTTAACATTGCTGGCCAACCTGCGCATCACAGACCCCCAGCGTTTCTTCCTGACCTGCGTGTCTGGTGA
  GGCCGGAGCAGGGAGGAGCTCGGACCCGCCCCTGCTGCTGGAGAAGGATGATCGCATCGTGCGCACCTTC
  CCGCCTGGGCAGCCCCTGTACCTGGCACGCAACGGTTCTCACCAGGTCACGCTGAGGGGCTTCTCCAAGC
  CCTCGGATTTGGTAGGCGTCTTCTCCTGTGTGGGTGGTGCTGGCGCGCGGCGCACTCGAGTTCTCTATGT
  GCACAACAGCCCAGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGTGACACTGCT
  GTGCTTTCTGCCCATGTGCACAAGGAAAAGCAGACTGACGTGATCTGGAAGAACAACGGATCCTACTTCA
  ATACCCTAGACTGGCAAGAGGCCGATGACGGGCGTTTTCAACTGCAGCTCCAAAATGTACAGCCACCATC
  AAGTGGCATCTACAGTGCCACCTACCTAGAGGCCAGCCCCCTGGGCAGTGCCTTCTTTCGGCTCATCGTG
  CGAGGCTGTGGGGCTGGACGCTGGGGGCCAGGATGTGTCAAGGATTGCCCAGGCTGCCTGCATGGAGGTG
  TCTGTCATGACCATGATGGCGAATGTGTGTGTCCCCCAGGATTCACTGGCACCCGCTGTGAGCAGGCCTG
  CAGGGAGGGTCGTTTTGGACAGAGCTGTCAGGAACAGTGCCCAGGCACAGCAGGTTGTAGAGGTCTCACC
  TTCTGCCTCCCGGATCCCTATGGCTGTTCCTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAGGAAGCAT
  GTGCACCTGGTCATTTTGGGGCTGATTGTCGCCTCCAGTGCCAGTGTCAAAATGGTGGCACTTGTGACCG
  GTTCAGCGGCTGCGTCTGCCCCTCTGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGGATCCCCCAG
  ATCCTCAGTATGGCCACAGAGGTGGAGTTCAACATAGGGACGATGCCCCGGATCAACTGTGCAGCTGCCG
  GGAATCCCTTCCCAGTGCGGGGCAGCATGAAACTTCGCAAGCCAGATGGCACCATGCTTTTGTCTACCAA
  AGTCATTGTGGAGCCAGACAGGACCACAGCAGAGTTTGAAGTGCCCAGTTTGACTCTTGGGGACAGTGGG
  TTCTGGGAATGCCGCGTATCGACTTCTGGTGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTCAAAGTAC
  CCCCAGTGCCTTTGACTGCACCTCGACTCCTGGCCAAGCAGAGCCGTCAGCTTGTGGTCTCCCCACTGGT
  CTCCTTTAGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGCACGATTGCC
  TGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAAGACAGGATACA
  ATGTCCGAGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGGCTGGGGACCTTCTGCCCTTATGAC
  TACTGACTGTCCTGAGCCTTTGCTGCAGCCCTGGCTAGAGAGCTGGCACGTGGAGGGTCCTGACAGGCTC
  CGTGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCCGGTGATGGTTTCCTGCTGCGCCTGTGGGATGGGG
  CCCGGGGACAGGAGAGGCGGGAGAACATCTCATTCCCCCAGGCCCGCACTGCCCTCCTGACTGGACTCAC
  GCCGGGCACCCACTACCAGCTGGATGTGCGACTGTATCACTGTACCCTCCTGGGCCCTGCCTCACCCCCT
  GCGCATGTGCACCTGCCCCCCAGCGGTAGGCCCACTCTGGGAAGGATGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209734 representing BC060182
  Red=Cloning site Green=Tags(s)
  
  MVWWGSSLLLPTLFLASHVGASVDLTLLANLRITDPQRFFLTCVSGEAGAGRSSDPPLLLEKDDRIVRTF
  PPGQPLYLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVLYVHNSPGAHLFPDKVTHTVNKGDTA
  VLSAHVHKEKQTDVIWKNNGSYFNTLDWQEADDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFRLIV
  RGCGAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCRGLT
  FCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQ
  ILSMATEVEFNIGTMPRINCAAAGNPFPVRGSMKLRKPDGTMLLSTKVIVEPDRTTAEFEVPSLTLGDSG
  FWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFSGDGPISSVRLHYRPQDSTIA
  WSAIVVDPSENVTLMNLKPKTGYNVRVQLSRPGEGGEGGWGPSALMTTDCPEPLLQPWLESWHVEGPDRL
  RVSWSLPSVPLSGDGFLLRLWDGARGQERRENISFPQARTALLTGLTPGTHYQLDVRLYHCTLLGPASPP
  AHVHLPPSGRPTLGRMG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060182
• ORF Size: 1941 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC060182.1
• RefSeq Size: 4259
• RefSeq ORF: 1943
• Locus ID: 21846