Btk (NM_013482) Mouse Tagged ORF Clone

CAT#: MG209829

Btk (GFP-tagged) - Mouse Bruton agammaglobulinemia tyrosine kinase (Btk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Btk
• Synonyms: AI528679; xid
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209829 representing NM_013482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGTGATACTGGAGAGCATCTTTCTGAAGCGCTCCCAGCAGAAAAAGAAAACATCACCTTTAA
  ACTTCAAGAAGCGCCTGTTTCTCTTGACTGTACACAAACTTTCATACTATGAATATGACTTTGAACGTGG
  GAGAAGAGGCAGTAAGAAAGGTTCAATAGATGTTGAGAAGATCACCTGTGTTGAAACAGTAATTCCTGAA
  AAAAATCCCCCACCAGAAAGACAGATTCCGAGGAGAGGTGAGGAGTCTAGTGAAATGGAACAGATTTCAA
  TCATTGAAAGGTTCCCGTACCCATTCCAGGTTGTATATGATGAAGGACCTCTCTATGTTTTCTCCCCAAC
  TGAAGAGCTGAGAAAGCGCTGGATTCACCAGCTCAAAAATGTAATCCGGTGCAATAGTGACCTGGTACAG
  AAATACCATCCTTGCTTCTGGATTGATGGACAGTATCTCTGCTGCTCTCAGACAGCCAAGAATGCTATGG
  GCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGGAGTTCTCATCGAAAAACGAAAAAGCC
  TCTTCCCCCTACCCCAGAGGAAGATCAGATCTTGAAAAAACCGCTTCCCCCGGAGCCAACAGCAGCACCA
  ATCTCCACAACCGAGCTGAAAAAGGTCGTGGCCCTTTATGATTACATGCCAATGAACGCAAATGACTTAC
  AATTGCGAAAGGGCGAGGAGTATTTTATCCTGGAGGAGAGCAACCTACCGTGGTGGCGAGCACGAGATAA
  AAATGGGCAGGAAGGCTACATCCCAAGTAACTATATCACTGAAGCTGAGGACTCCATAGAGATGTATGAG
  TGGTATTCCAAGCACATGACTCGAAGTCAAGCTGAGCAACTGCTAAAGCAAGAGGGGAAAGAAGGAGGTT
  TCATTGTCGGAGACTCCAGCAAAGCTGGAAAATACACCGTGTCTGTGTTTGCTAAATCTACTGGGGAGCC
  TCAAGGGGTGATCCGCCATTACGTTGTGTGTTCCACGCCACAGAGCCAGTATTACCTGGCTGAGAAACAC
  CTCTTCAGCACCATCCCTGAGCTCATTAACTACCATCAACACAACTCTGCAGGCCTCATATCCAGGCTGA
  AATATCCTGTGTCTAAACAAAACAAAAACGCGCCTTCTACTGCAGGCCTGGGCTATGGATCATGGGAAAT
  TGATCCAAAGGACCTCACCTTCTTGAAGGAGCTTGGGACTGGACAATTCGGTGTCGTGAAATATGGGAAG
  TGGAGGGGCCAATATGATGTGGCCATCAAGATGATCAGAGAAGGTTCCATGTCGGAGGATGAATTCATTG
  AAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTGGTGCAGTTGTATGGCGTCTGCACCAAACA
  ACGCCCCATCTTCATCATCACCGAGTACATGGCTAATGGCTGCCTCTTGAACTACCTGAGGGAGATGCGG
  CACCGCTTCCAGACACAGCAGCTGCTTGAGATGTGCAAAGATGTCTGTGAAGCAATGGAATACTTGGAGT
  CGAAGCAGTTCCTTCACAGAGACCTGGCAGCTCGAAACTGTTTGGTAAACGATCAAGGAGTTGTGAAAGT
  ATCTGACTTTGGCCTGTCTAGGTATGTCCTTGATGATGAGTACACCAGCTCTGTAGGCTCCAAGTTTCCA
  GTCCGGTGGTCTCCACCAGAAGTGCTTATGTATAGCAAGTTCAGCAGCAAATCTGACATCTGGGCTTTTG
  GGGTTTTAATGTGGGAGATCTACTCCCTGGGGAAGATGCCGTATGAGAGATTTACTAACAGTGAGGCAGC
  AGAACACATTGCTCAAGGCTTACGTCTCTACAGGCCTCATCTGGCATCAGAGAGGGTATATACCATCATG
  TACAGCTGCTGGCACGAGAAAGCAGATGAACGTCCTAGTTTCAAAATTCTCTTGAGTAACATTCTAGATG
  TGATGGATGAAGAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209829 representing NM_013482
  Red=Cloning site Green=Tags(s)
  
  MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVIPE
  KNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRCNSDLVQ
  KYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPTAAP
  ISTTELKKVVALYDYMPMNANDLQLRKGEEYFILEESNLPWWRARDKNGQEGYIPSNYITEAEDSIEMYE
  WYSKHMTRSQAEQLLKQEGKEGGFIVGDSSKAGKYTVSVFAKSTGEPQGVIRHYVVCSTPQSQYYLAEKH
  LFSTIPELINYHQHNSAGLISRLKYPVSKQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGK
  WRGQYDVAIKMIREGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMR
  HRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFP
  VRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSEAAEHIAQGLRLYRPHLASERVYTIM
  YSCWHEKADERPSFKILLSNILDVMDEES
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013482
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013482.1, NP_038510.1
• RefSeq Size: 2485
• RefSeq ORF: 1980
• Locus ID: 12229