Ski (BC068305) Mouse Tagged ORF Clone

CAT#: MG209957

Ski (GFP-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ski
• Synonyms: MGC8300
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209957 representing BC068305
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCTGCAGCGGCCGGCCGGGGCGGCTTCCAGCCGCACCCGGGGCTGCAGAAGACGCTGGAGCAGT
  TCCACCTGAGCTCCATGAGCTCGCTGGGCGGCCCGGCCGCTTTCTCGGCGCGCTGGGCGCAGGAGGCCTA
  CAAGAAGGAGACCGTGCTGGAAGGGGAGACCATCTCTTGTTTCGTGGTGGGAGGTGAGAAGCGTCTGTGC
  CTGCCGCAGATCCTCAACTCGGTGCTGCGCGACTTCTCACTGCAGCAGATCAACTCCGTGTGCGATGAGC
  TGCACATCTACTGCTCGCGCTGCACCGCCGACCAGCTGGAGATCCTCAAAGTCATGGGCATCCTGCCCTT
  CTCCGCGCCCTCCTGCGGGCTCATCACCAAGACGGACGCCGAGCGCCTGTGCAACGCGCTGCTCTACGGC
  GGCGCCTACCCGCCGCCTTGCAAGAAGAAGTTGGCGGCCAGCCTGGCGCTGGGCCTGGAGCTCAGCGAAC
  GCAGCGTCCGCGTGTACCACGAGTGCTTCGGCAAGTGCAAAGGGCTGCTGGTGCCCGAGCTGTACAGCAG
  TCCCAGCGCTGCCTGCATCCAGTGCCTCGACTGCCGCCTCATGTACCCGCCGCACAAGTTCGTTGTACAC
  TCACACAAGGCCCTGGAGAACCGGACCTGCCACTGGGGCTTCGACTCAGCCAACTGGCGGGCCTACATCC
  TGCTGAGCCAGGACTACACGGGCAAGGAGGAGCAGGCCCGCCTTGGCCGCTGCCTGGACGACGTGAAGGA
  GAAGTTCGACTATGCCAACAAGTACAAGCGGAGGGTGCCCCGGGTCTCAGAGCCCCCAGCTTCCATAAGA
  CCCAAAACAGACGACACTTCCTCACAGTCCCCTGCGTCTTCTGAGAAGGACAAGCAGTCCACTTGGCTGC
  GGACCTTGGCTGGTTCCTCCAATAAGAGCCTCGGCTGCACTCACCCTCGCCAGCGCCTTTCTGCTTTCCG
  GCCCTGGTCCCCTGCAGTGTCTGCGAGTGAGAAAGAGACGTCCCCACACCTCCCAGCTTTGATTCGAGAC
  AGCTTCTACTCCTACAAGAGCTTTGAGACAGCCGTGGCCCCCAATGTGGCCCTAGCACCACCAACCCAGC
  AAAAGGTTGTGAACAGTCCACCCTGCACCACCGTGGTCTCCCGGGCACCAGAGCCTCTTACTACTTGTAT
  CCAGCCACGAAAACGGAAGCTGACTCTGGACACAGCAGGAGCCCCAGACATGCTGACACCTGTGGCTGCT
  GCAGAGGAAGACAAGGATTCTGAGGCCGAGGTTGAAGTTGAAAGCAGAGAGGAGTTCACCTCCTCCTTGT
  CCTCGCTGTCCTCTCCGTCCTTTACCTCGTCCAGCTCTGCCAAGGATCTAAGCTCCCCAGGCATGCATGC
  TCCGCCCGTGGTTGCCCCCGATGCTGCAGCCCATGTTGATGCCCCAAGTGGGCTGGAGGCAGAGTTGGAG
  CACCTGCGGCAAGCCCTGGAGGGTGGCCTGGATACTAAGGAAGCCAAAGAAAAGTTCCTGCATGAGGTGG
  TCAAAATGCGAGTTAAGCAAGAGGAAAAGCTGACAGCAGCCCTGCAGGCTAAGCGCAGCCTGCACCAGGA
  GCTAGAATTCTTGCGTGTGGCCAAGAAGGAGAAACTGCGGGAAGCCACAGAGGCCAAGCGCAGTTTGAGG
  AAAGAGATTGAGCGGCTCCGAGCTGAAAACGAGAAGAAGATGAAAGAGGCCAACGAGTCCCGGGTGCGCC
  TGAAGCGAGAGCTGGAGCAGGCGCGGCAGGTCCGGGTGTGTGACAAGGGCTGCGAGGCCGGGCGCCTGCG
  TGCCAAGTACTCAGCCCAGATTGAGGACCTGCAAGCGAAGCTGCAGCATGCGGAGGCCGACCGCGAGCAG
  CTTCGAGCTGACCTGCTGCGGGAACGTGAGGCCCGCGAGCATCTGGAGAAGGTGGTGAGGGAGCTTCAGG
  AGCAGCTGCGGCCTCGGCCACGGCCTGAGCATCCTGGAGGTGAGAGCAACGCTGAGCTGGGTCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209957 representing BC068305
  Red=Cloning site Green=Tags(s)
  
  MEAAAAGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKETVLEGETISCFVVGGEKRLC
  LPQILNSVLRDFSLQQINSVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYG
  GAYPPPCKKKLAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVH
  SHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYANKYKRRVPRVSEPPASIR
  PKTDDTSSQSPASSEKDKQSTWLRTLAGSSNKSLGCTHPRQRLSAFRPWSPAVSASEKETSPHLPALIRD
  SFYSYKSFETAVAPNVALAPPTQQKVVNSPPCTTVVSRAPEPLTTCIQPRKRKLTLDTAGAPDMLTPVAA
  AEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLSSPGMHAPPVVAPDAAAHVDAPSGLEAELE
  HLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLTAALQAKRSLHQELEFLRVAKKEKLREATEAKRSLR
  KEIERLRAENEKKMKEANESRVRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQAKLQHAEADREQ
  LRADLLREREAREHLEKVVRELQEQLRPRPRPEHPGGESNAELGP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC068305
• ORF Size: 2027 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC068305, AAH68305
• RefSeq Size: 3912
• RefSeq ORF: 2027
• Locus ID: 20481