Gbe1 (NM_028803) Mouse Tagged ORF Clone

CAT#: MG210139

Gbe1 (GFP-tagged) - Mouse glucan (1,4-alpha-), branching enzyme 1 (Gbe1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gbe1
• Synonyms: 2310045H19Rik; 2810426P10Rik; D16Ertd536e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210139 representing NM_028803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCGGCGGCTCCTGCGGCAGGGGAAACGGGCCCCGATGCTCGTCTGGAGGCCGCCTTGGCCG
  ACGTGCCGGAGCTGGCCCGACTCCTGGAGATTGACCCGTACCTGAAGCCCTTCGCCGCGGACTTCCAGCG
  CAGGTATAAGAAGTTTAGCCAGGTTTTGCATGACATTGGAGAGAATGAAGGTGGGATCGATAAGTTCTCC
  AGAGGCTATGAGTCATTTGGCATCCACAGATGTTCTGATGGTGGCATCTACTGCAAAGAATGGGCCCCAG
  GAGCAGAAGGAGTTTTTCTTACTGGAGAATTCAGTGGTTGGAATCCATTTTCTCACCCATATAAAAAGCT
  GGAATACGGAAAATGGGAGCTGTATATCCCACCCAAGCAGAACAAATCCCCCCTGATACCTCATGGGTCC
  AAGCTGAAGGTAGTTATTACTAGTAAGAGTGGCGAGATACTGTATCGCATTTCCCCATGGGCAAAGTATG
  TGGTTCGTGAGAACAACAACGTGAACTATGATTGGATACACTGGGCTCCAGAAGACCCCTATAAATTCAA
  GCATTCCAGACCTAAGAAACCAAGAAGCCTAAGAATTTATGAATCTCATGTGGGAATTTCTTCCCACGAA
  GGAAAAATAGCTTCTTACAAACATTTTACCAGCAATGTACTACCAAGAATCAAAGACCTTGGGTATAACT
  GCATTCAGTTGATGGCGATCATGGAACATGCTTACTATGCCAGTTTTGGGTACCAAATCACGAGCTTTTT
  TGCAGCTTCAAGTCGTTATGGAACTCCTGAAGAGCTGAAAGAACTTGTTGACACAGCACATTCGATGGGC
  ATTGTAGTCCTCCTAGATGTGGTTCATAGCCATGCTTCAAAAAATTCAGAAGATGGGTTAAACATGTTTG
  ATGGGACCGATTCCTGTTATTTTCACTCTGGACCCCGAGGGACCCATGATCTTTGGGACAGTAGATTATT
  TATCTATTCCAGCTGGGAAGTTTTAAGATTCCTTCTGTCGAACATAAGATGGTGGTTGGAGGAGTACTGC
  TTTGATGGCTTCCGTTTTGATGGTGTCACCTCTATGCTCTATCATCACCACGGAATGGGTCAAGGTTTTT
  CGGGTGACTATAATGAATATTTTGGACTACAAGTAGATGAAGATGCTTTGATTTATCTCATGTTGGCAAA
  TCATTTGGCTCACACGTTGTACCCAGACTCGATAACAATAGCAGAGGATGTATCAGGGATGCCGGCTCTC
  TGTTCTCCAACTTCCCAGGGAGGCGGTGGTTTTGACTACAGATTAGCAATGGCTATTCCAGATAAATGGA
  TCCAATTACTTAAGGAATTTAAAGATGAAGACTGGAATATGGGCAATATAGTGTATACTCTCACCAATCG
  ACGCTACCTTGAAAAATGTGTGGCTTATGCAGAGAGTCATGATCAGGCATTGGTTGGTGACAAGACACTG
  GCGTTTTGGTTGATGGACGCTGAGATGTACACGAACATGAGCGTTCTGGCCCCTTTCACTCCAGTAATTG
  ATCGAGGAATACAGCTTCATAAGATGATTCGTCTCATCACTCACGGGCTCGGTGGAGAAGGCTATCTCAA
  TTTCATGGGTAATGAGTTTGGGCATCCTGAATGGTTGGACTTCCCAAGGAAAGGAAATAATGAGAGTTAC
  CATTATGCCAGAAGGCAGTTTAATTTAACCGACGATGACCTTCTTCGCTATAAGTTCCTAAATAACTTTG
  ACAGAGATATGAATAGACTGGAAGAAAGATGTGGTTGGCTTTCAGCTCCACAGGCCTACGTGAGTGAAAA
  ACATGAAGCCAATAAGACAATCACCTTTGAGAGAGCAGGACTTCTCTTTATTTTCAACTTCCACCCAAGC
  AAGAGCTATACGGACTACCGAGTCGGGACAGCAACACCAGGGAAGTTCAAAATTGTACTAGATTCTGACG
  CAGCGGAGTATGGAGGTCATCAGAGACTGGACCACAACACCAACTACTTTGCTGAGGCTTTTGAACATAA
  TGGGCGCCCCTATTCTCTTCTGGTGTACATTCCAAGCCGAGTGGCTCTCATCCTTCAGAATGTGGATCTG
  CAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210139 representing NM_028803
  Red=Cloning site Green=Tags(s)
  
  MAAPAAPAAGETGPDARLEAALADVPELARLLEIDPYLKPFAADFQRRYKKFSQVLHDIGENEGGIDKFS
  RGYESFGIHRCSDGGIYCKEWAPGAEGVFLTGEFSGWNPFSHPYKKLEYGKWELYIPPKQNKSPLIPHGS
  KLKVVITSKSGEILYRISPWAKYVVRENNNVNYDWIHWAPEDPYKFKHSRPKKPRSLRIYESHVGISSHE
  GKIASYKHFTSNVLPRIKDLGYNCIQLMAIMEHAYYASFGYQITSFFAASSRYGTPEELKELVDTAHSMG
  IVVLLDVVHSHASKNSEDGLNMFDGTDSCYFHSGPRGTHDLWDSRLFIYSSWEVLRFLLSNIRWWLEEYC
  FDGFRFDGVTSMLYHHHGMGQGFSGDYNEYFGLQVDEDALIYLMLANHLAHTLYPDSITIAEDVSGMPAL
  CSPTSQGGGGFDYRLAMAIPDKWIQLLKEFKDEDWNMGNIVYTLTNRRYLEKCVAYAESHDQALVGDKTL
  AFWLMDAEMYTNMSVLAPFTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEWLDFPRKGNNESY
  HYARRQFNLTDDDLLRYKFLNNFDRDMNRLEERCGWLSAPQAYVSEKHEANKTITFERAGLLFIFNFHPS
  KSYTDYRVGTATPGKFKIVLDSDAAEYGGHQRLDHNTNYFAEAFEHNGRPYSLLVYIPSRVALILQNVDL
  QN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028803
• ORF Size: 2106 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028803.3, NP_083079.1
• RefSeq Size: 2846
• RefSeq ORF: 2109
• Locus ID: 74185