Ext1 (NM_010162) Mouse Tagged ORF Clone

CAT#: MG210422

Ext1 (GFP-tagged) - Mouse exostoses (multiple) 1 (Ext1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ext1
• Synonyms: AA409028
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210422 representing NM_010162
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCCAAAAAACGCTATTTCATCCTGCTCTCAGCTGGCTCTTGTCTCGCCCTTTTGTTTTATTTTG
  GAGGCGTGCAGTTTAGGGCATCGAGGAGCCACAGCCGGAGAGAAGAGCACAGTGGTCGGAATGGCTTGCA
  CCAGCCCAGTCCGGATCATTTCTGGCCCCGCTTCCCGGACGCTCTGCGCCCTTTCTTTCCTTGGGATCAA
  TTGGAAAACGAGGATTCCAGCGTGCACATTTCCCCCCGGCAGAAGCGAGACGCCAACTCGAGCATCTACA
  AAGGCAAGAAGTGCCGCATGGAGTCCTGCTTCGATTTCACCCTTTGCAAGAAAAACGGCTTCAAAGTCTA
  CGTGTACCCGCAGCAGAAAGGGGAGAAAATCGCCGAAAGTTACCAAAACATTCTAGCGGCCATCGAGGGC
  TCCAGGTTCTACACCTCGGACCCCAGCCAGGCGTGCCTCTTTGTCTTGAGTCTGGATACTTTAGACAGAG
  ACCAGTTATCACCTCAGTATGTGCACAATTTGAGATCCAAAGTGCAGAGTCTCCACTTGTGGAACAATGG
  TAGGAATCATTTAATTTTTAATTTATATTCTGGCACTTGGCCTGACTACACTGAGGACGTGGGGTTTGAC
  ATCGGCCAGGCGATGCTGGCCAAAGCCAGCATCAGTACTGAAAACTTCCGACCAAACTTTGATGTTTCTA
  TTCCCCTCTTTTCTAAGGATCATCCCAGGACAGGAGGGGAGAGGGGGTTTTTGAAATTTAACACCATCCC
  TCCTCTCAGGAAGTACATGCTGGTATTCAAGGGGAAGCGGTACCTGACAGGGATAGGGTCAGACACCAGG
  AATGCCTTATATCACGTCCATAACGGGGAGGACGTCTTGCTCCTCACCACCTGCAAGCATGGCAAAGACT
  GGCAAAAGCACAAGGATTCTCGCTGTGACAGAGACAACACCGAGTATGAGAAATATGATTATCGGGAAAT
  GCTGCACAATGCCACTTTCTGTCTGGTTCCTCGTGGTCGCAGGCTTGGGTCCTTCAGATTCCTGGAGGCT
  TTGCAGGCTGCCTGTGTCCCTGTAATGCTCAGCAACGGATGGGAGTTGCCATTCTCCGAAGTGATTAATT
  GGAACCAAGCTGCCGTCATAGGCGATGAGAGATTGCTATTACAGATTCCTTCTACAATCAGGTCTATTCA
  TCAGGATAAAATCCTAGCACTTAGACAGCAGACACAGTTCTTGTGGGAGGCTTATTTTTCTTCAGTTGAG
  AAGATTGTATTAACTACACTAGAGATTATTCAGGACAGAATATTCAAGCACATATCACGTAACAGTTTAA
  TATGGAACAAACATCCTGGAGGATTGTTCGTCCTACCGCAGTATTCATCTTACCTGGGAGATTTCCCTTA
  CTACTATGCTAATTTAGGTTTAAAGCCCCCCTCCAAATTCACTGCAGTCATCCATGCTGTGACTCCCCTG
  GTCTCTCAGTCCCAGCCAGTGTTGAAGCTTCTTGTGGCTGCAGCCAAATCCCAGTACTGTGCGCAGATCA
  TAGTTCTGTGGAATTGTGACAAGCCTCTACCAGCCAAACATCGCTGGCCTGCCACTGCCGTGCCTGTCAT
  CGTCATTGAAGGAGAAAGCAAGGTTATGAGCAGCCGGTTTCTGCCCTATGACAACATCATCACTGATGCT
  GTGCTCAGCCTGGATGAGGACACTGTGCTTTCAACTACGGAAGTGGATTTTGCCTTCACCGTATGGCAGA
  GCTTCCCAGAGAGGATTGTGGGATATCCTGCTCGCAGTCATTTCTGGGATAACTCTAAGGAGCGGTGGGG
  ATATACATCCAAGTGGACGAATGACTACTCCATGGTGTTGACAGGAGCTGCTATCTACCACAAATATTAT
  CACTACCTGTATTCCCATTACCTGCCAGCCAGCCTGAAGAACATGGTAGACCAACTGGCCAACTGTGAGG
  ACATTCTCATGAATTTCCTGGTGTCTGCTGTGACAAAATTGCCTCCAATCAAAGTGACCCAGAAGAAACA
  GTATAAGGAGACAATGATGGGACAGACTTCCCGAGCATCCCGCTGGGCCGACCCTGACCACTTTGCCCAG
  CGACAGAGCTGCATGAATACATTTGCCAGCTGGTTTGGCTACATGCCGCTGATCCATTCTCAGATGAGGC
  TGGACCCGGTCCTCTTTAAAGACCAAGTCTCAATTCTGAGGAAGAAATACAGAGACATTGAACGACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210422 representing NM_010162
  Red=Cloning site Green=Tags(s)
  
  MQAKKRYFILLSAGSCLALLFYFGGVQFRASRSHSRREEHSGRNGLHQPSPDHFWPRFPDALRPFFPWDQ
  LENEDSSVHISPRQKRDANSSIYKGKKCRMESCFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEG
  SRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFD
  IGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTR
  NALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEA
  LQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVE
  KIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPL
  VSQSQPVLKLLVAAAKSQYCAQIIVLWNCDKPLPAKHRWPATAVPVIVIEGESKVMSSRFLPYDNIITDA
  VLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYY
  HYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQ
  RQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010162
• ORF Size: 2238 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010162.2, NP_034292.2
• RefSeq Size: 3478
• RefSeq ORF: 2241
• Locus ID: 14042