Lima1 (BC031490) Mouse Tagged ORF Clone

CAT#: MG210477

Lima1 (GFP-tagged) - Mouse LIM domain and actin binding 1 (cDNA clone MGC:27894 IMAGE:3498138)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lima1
• Synonyms: Eplin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210477 representing BC031490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCGACTCCATTTAATAGACGCCAGTGGACTTCCCTGTCATTGAGAGTAACAGCCAAAGAGCTTT
  CTCTTGTCAACAAGAACAAGTCATCCGCAATTGTGGAAATATTCTCCAAGTACCAGAAGGCTGCTGAAGA
  AGCCAACATGGAAAGGAAGAAAAATAACCCTGAAAGTCTGCCCCAGCACTTTAGAAGAGGGACCCTGTCT
  GTGTTAAAGAAGAAGTGGGAGAACCCGGTGGCTGGGGCAGAATTCCACACAGACTCACTGCCAAACAGCA
  GCAGTGAGGGTGGGCACACAGCGGACTACCCTCCTGCTGAAGTGACGGACAAGCCTGCTCCTGGAGTCAG
  AGCTGACCGGGAAGAGCACACCCAGCCCAAACCTAGATTTGGATCTCGTCCGGAAGCAGTTATCCAGAGC
  CGGTATCCTCGCTCAGAGAACAGCCACGATTTTAAAGCCCAGGCCACGGAGAGCCAAAAAATGGAAAACT
  GTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCAGAGACGAGCGAAAACACAGAAACTTCAGGCAAAAT
  AGAGAAATACAACGTTCCACTGAATAGACTGAAGATGATGTTTGAGAAAGGTGAACACAACCAAACCAAG
  AGTCTCTGGACCCAAAGCCGAAATGCGGGTGGAAGGAGGCTCTCTGAAAACAACTGTTCCCTGGATGACT
  GGGAAATAGGTGCGGGTCATTTGTCTTCCTCTGCATTCAACTCGGAGAAAAACGAGAGTAAGAGGAATCT
  GGAGCTGCCACGCCTCTCAGAAACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGCAGTGTCCAAG
  CAGAGCAGCCCAGCCAGCTACACAAATGAGCTGAAAACCAGTGAAAGCAAAACTCATAAATGGGAACAGA
  AGGAGAATGTGCCCCCAGGTCCCGAGGCCTGCAGCGTCCATCAGGAAGGAAGCAAGGTTTCTACAACTGA
  GAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCTGTAACTCCCAGGTGAAGAGCGAGGCC
  CAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAAAGTTCTCCTT
  CCAAAACAGCGAAGAAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGACGGTGTACCC
  CATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTACTGCAACAAC
  AAGCTCAGTCTAGGAACATATGCATCCTTACATGGACGAATCTACTGTAAGCCTCACTTCAATCAACTCT
  TCAAGTCCAAAGGCAACTATGACGAGGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGGCAAGCAAGAG
  CGACAATGAGGAGACTTTGGGGAGACCAGCCCAGCCGCCTAATGCAGGGGAGAGCCCCCATAGCCCGGGT
  GTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCGGCAAGTATGGAAGCCAAGGCCTCTTCTCAGA
  GGGAGAGGGAGGATAAGCCTGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCGCCAGCCGAGCTGGG
  CGGTTCCGGAAGTGCCCTGGAGGAAGGGATCAAAGTATCGAAGCCCAAGTGGCCTCCGGAGGATGACGTC
  TGCAAGACGGAGGCCCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGCGGCGGTCTTCGTCCCTGAAGG
  AGAGGAGCCGCCCGTTTACGGTAGCAGCTTCCTTTCGAACCTCTTCCATCAAGAGCCCCAAGGCCTCTTC
  GCCATCTCTCAGGAAAGGTTGGAGCGAGTCCGAGCAGAGTGAGGAGTTTGGAGGAGGGATAGCGACGATG
  GAAAGGAAACAAACGGAAAATGCCAGACCCTCTGGGGAGAAAGAGAATGTGGGAAAATCACGCTGGCAGG
  GCGAGGAAGTGCCGAGGAGCAAGGACCGTAGTTCTTTTGAGCTGGAGAGTGAGAATTTTATGGAAAATGG
  AGCAAACATAGCTGAAGATGACAACCATGTCCACGCACAGCAGTCCCCGTTAGAACCCGAGGCTCCGGGT
  TGGTCTGGCTTTGTAGACACCACGGCTGCTAAAGAATTCACTACCCAGAATCAAAAATCCCAAGATGTCG
  GGTTCTGGGAGGGAGAAGTGGTCCGAGAGCTGTCTGTGGAGGAACAGATAAAGAGAAACCGGTACTATGA
  TGAGGACGAGGATGAAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210477 representing BC031490
  Red=Cloning site Green=Tags(s)
  
  MESTPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEEANMERKKNNPESLPQHFRRGTLS
  VLKKKWENPVAGAEFHTDSLPNSSSEGGHTADYPPAEVTDKPAPGVRADREEHTQPKPRFGSRPEAVIQS
  RYPRSENSHDFKAQATESQKMENCLGDSRHEAEKPETSENTETSGKIEKYNVPLNRLKMMFEKGEHNQTK
  SLWTQSRNAGGRRLSENNCSLDDWEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQAAVSK
  QSSPASYTNELKTSESKTHKWEQKENVPPGPEACSVHQEGSKVSTTENSLVALSVPAEDDTCNSQVKSEA
  QQPMHPKPLSPDARTSSLPESSPSKTAKKFQAPAKESCVECQKTVYPMERLLANQQVFHISCFRCSYCNN
  KLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHKQHKDLWASKSDNEETLGRPAQPPNAGESPHSPG
  VEDAPIAKVGVLAASMEAKASSQREREDKPAETKKLRIAWPPPAELGGSGSALEEGIKVSKPKWPPEDDV
  CKTEAPEDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSIKSPKASSPSLRKGWSESEQSEEFGGGIATM
  ERKQTENARPSGEKENVGKSRWQGEEVPRSKDRSSFELESENFMENGANIAEDDNHVHAQQSPLEPEAPG
  WSGFVDTTAAKEFTTQNQKSQDVGFWEGEVVRELSVEEQIKRNRYYDEDEDEE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031490
• ORF Size: 2261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC031490, AAH31490
• RefSeq Size: 3232
• RefSeq ORF: 2261
• Locus ID: 65970