Dclk2 (NM_027539) Mouse Tagged ORF Clone

CAT#: MG210491

Dclk2 (GFP-tagged) - Mouse doublecortin and CaM kinase-like 2 (Dcamkl2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dclk2
• Synonyms: 6330415M09Rik; AU044875; CL2; Click-II; CLICK2; Dcamkl2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210491 representing NM_027539
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCACAAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCAC
  GGAGAGGGGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG
  CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG
  GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTATTTCAAAGGCCTGGTCTTTGCCATCTCCAACG
  ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCACTGTCTGACAATGTGAACCTGCC
  CCAGGGCGTCCGCACCATCTACACCATAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA
  GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTTCGTAAAGTTGATTACACCAAAAACGTTAATCCGA
  ACTGGTCTGTGAACATCAAGGGCGGAACCACCCGCACCCTGGCCGTGGCCTCTGCGAAGAGTGAGGTGAA
  AGAAAGCAAAGACTTCATTAAACCCAAGTTAGTGACTGTGATTCGAAGCGGAGTGAAGCCTAGAAAGGCC
  GTGCGGATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCTTAACAGATATCACCGAAGCCA
  TTAAACTAGACTCAGGCGTGGTCAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA
  CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT
  GTCCTGGATCATAGCGAATGCCGTGTCCTGAAATCGTCCTACTCTCGAGCCTCAGCTGCGAAGTATTCTG
  GATCCAGAAGCCCAGGGTTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTGAATGGAACTCCCAGTAGCCA
  GCTTTCCACTCCGAAGTCCACCAAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGATTG
  AAGCAGATTTCTGCTCAGGGCAGATCTTCTTCCAACGTAAACGGTGGGCCTGAACTTGACCGTTGCCTGA
  GCCCTGAAGGTGTGAATGGAAACCGGTGCTCCGAGTCGTTCCCCCTTCTGGAGAAATACAGAATAGGGAA
  GGTCATCGGGGACGGCAACTTCGCGGTAGTTAAGGAGTGCGTGGACAGGTACACTGGAAAAGAGTTTGCA
  TTAAAGATTATAGACAAAGCCAAATGCTGTGGAAAGGAGCATCTGATTGAGAACGAAGTGTCAATCCTGC
  GCCGAGTGAAGCACCCCAACATCATCATGTTGGTTGAAGAGATGGAAACAGCAACTGACCTCTTTCTAGT
  GATGGAACTGGTCAAAGGTGGAGATCTCTTTGATGCGATTACCTCTTCAACCAAGTACACTGAGAGAGAT
  GGAAGCGCCATGGTGTACAACCTAGCCAATGCCCTCCGGTACCTGCACAGCCTCAGCATCGTCCACAGGG
  ACATCAAGCCTGAGAATCTGCTGGTGTGCGAATACCCAGATGGAACCAAGTCTTTGAAGCTGGGAGACTT
  TGGGCTGGCGACGGTGGTTGAAGGCCCGTTGTACACGGTCTGTGGCACGCCAACTTATGTGGCACCAGAG
  ATCATAGCTGAAACAGGTTATGGCCTGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACATACATACTTC
  TCTGTGGATTCCCACCATTCCGGAGTGAGAACAATCTCCAGGAAGATCTCTTTGACCAGATCTTGGCTGG
  AAAGCTGGAATTCCCAGCCCCCTACTGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATG
  CTTCAGGTAAACGTTGAAGCTCGCTGTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATG
  CATCCCAGGAGAACAATATGCAAGCCGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCC
  CAAACAGAACAGCACCACCACCGGGGTCTCCGTTATCATGAACACGGCTCTAGATAAAGAGGGGCAGATT
  TTCTGCAGCAAGCTCTGTCAAGACAGCAGCAGACCATCCAGGGAGCAGACCTCGCCAGTCCCTCCCTCAG
  CCCAGGAGGCCCCTCCCCCACTGGAGTCTCCCAGGCCCCCTGGTCCTCCAGCCACCTCTGGCTGTGATCT
  GGCAGGGACCTGGCGCCGCCACCGAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210491 representing NM_027539
  Red=Cloning site Green=Tags(s)
  
  MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK
  AKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLE
  GESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKA
  VRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDF
  VLDHSECRVLKSSYSRASAAKYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGL
  KQISAQGRSSSNVNGGPELDRCLSPEGVNGNRCSESFPLLEKYRIGKVIGDGNFAVVKECVDRYTGKEFA
  LKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERD
  GSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPE
  IIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQM
  LQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMNTALDKEGQI
  FCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHRD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027539
• ORF Size: 2268 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027539.3
• RefSeq Size: 4032
• RefSeq ORF: 2271
• Locus ID: 70762
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. This gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]