Mfn2 (NM_133201) Mouse Tagged ORF Clone

CAT#: MG210494

Mfn2 (GFP-tagged) - Mouse mitofusin 2 (Mfn2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mfn2
• Synonyms: D630023P19Rik; Fzo
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210494 representing NM_133201
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTGCTCTTTTCTCGATGCAACTCCATCGTCACCGTCAAGAAGGATAAGCGACACATGGCTGAAG
  TGAATGCTTCCCCTCTCAAGCACTTTGTCACTGCCAAGAAAAAGATCAATGGAATCTTTGAGCAGCTGGG
  GGCCTACATCCAAGAGAGCGCCAGCTTCCTTGAAGACACCCACAGGAACACAGAACTGGACCCGGTTACC
  ACGGAAGAGCAGGTCCTGGACGTCAAAGGGTACCTGTCCAAGGTCAGGGGTATCAGCGAAGTGCTGGCCA
  GGCGGCACATGAAGGTGGCTTTTTTTGGCCGGACGAGCAATGGGAAGAGCACCGTGATCAATGCCATGCT
  CTGGGACAAAGTTCTGCTATCTGGGATTGGTCATACCACCAATTGCTTCCTGCGGGTTGGGGGCACAGAT
  GGCCATGAGGCCTTCCTCCTCACAGAGGGCTCAGAAGAGAAGAAGAGTGTCAAGACTGTGAACCAACTGG
  CCCATGCCCTCCATCAGGACGAGCAGTTGCATGCAGGCAGCATGGTGAGTGTGATGTGGCCCAACTCCAA
  GTGTCCGCTCCTGAAGGATGACCTCGTGCTGATGGACAGCCCTGGGATCGATGTTACCACGGAGCTGGAC
  AGCTGGATTGATAAGTTTTGCCTGGATGCTGATGTGTTTGTGCTGGTGGCCAACTCAGAGTCCACGCTGA
  TGCAGACGGAGAAGCAGTTCTTCCACAAAGTGAGTGAACGTCTCTCCCGGCCCAACATCTTCATCCTGAA
  CAACCGCTGGGATGCGTCTGCCTCGGAGCCTGAGTACATGGAGGAGGTGCGGCGGCAGCACATGGAGCGC
  TGCACCAGCTTTCTGGTGGATGAGCTGGGCGTGGTGGATCGAGCTCAGGCTGGGGACCGGATCTTCTTCG
  TGTCTGCCAAGGAGGTTCTCAGCGCCAGGGTCCAGAAAGCCCAGGGCATGCCAGAAGGAGGCGGCGCTCT
  CGCAGAAGGTTTTCAAGTGAGGATGTTTGAGTTTCAGAATTTCGAGAGGCAGTTTGAGGAGTGCATTTCC
  CAGTCTGCAGTAAAGACCAAATTTGAGCAGCACACAGTCCGGGCCAAGCAGATTGCAGAGGCCGTTCGTC
  TCATCATGGATTCCCTGCACATCGCAGCTCAGGAGCAGCGGGTTTATTGCCTAGAAATGCGGGAAGAGCG
  GCAAGACCGGCTGAGGTTTATTGACAAGCAGCTGGAGCTCCTGGCTCAAGACTACAAGCTGCGAATTAAG
  CAGATTACGGAGGAAGTGGAAAGGCAGGTGTCCACAGCCATGGCTGAAGAGATCAGGCGCCTCTCTGTGC
  TAGTTGACGAGTACCAGATGGACTTCCACCCATCCCCAGTTGTCCTCAAGGTTTATAAGAACGAGCTGCA
  CCGCCATATAGAGGAAGGTCTGGGCCGGAACCTGTCTGACCGCTGCTCCACTGCCATTGCCAGTTCACTG
  CAGACTATGCAGCAGGACATGATAGACGGCTTGAAGCCCCTTCTTCCTGTATCTATGCGGAATCAGATAG
  ACATGCTGGTCCCTCGACAGTGTTTCTCCCTCAGCTATGACCTGAATTGTGACAAGCTGTGTGCTGACTT
  TCAGGAGGACATCGAGTTCCACTTCTCCCTTGGATGGACTATGCTAGTGAACAGGTTCCTGGGCCCCAAG
  AATAGCCGCCGGGCCTTGCTAGGCTACAGTGATCAGGTTCAGCGTCCTCTCCCTCTGACACCTGCCAACC
  CCAGCATGCCCCCCTTGCCACAGAGCTCCCTCACCCAGGAGGAGCTCATGGTCTCCATGGTTACTGGCCT
  GGCCTCTCTGACGTCTAGGACCTCCATGGGCATTCTTGTGGTCGGAGGAGTGGTGTGGAAGGCAGTGGGC
  TGGAGACTCATCGCCCTCTCCTTTGGACTGTATGGCCTCCTGTACGTCTATGAGCGACTGACCTGGACCA
  CCAAAGCCAAAGAGAGGGCCTTCAAGCGCCAGTTTGTGGAATACGCCAGTGAGAAGCTACAGCTCATCAT
  CAGTTACACCGGCTCTAACTGCAGCCACCAAGTCCAGCAGGAATTGTCTGGGACATTTGCTCATCTGTGC
  CAGCAAGTTGACATCACCCGAGATAATCTGGAGCAGGAAATTGCTGCCATGAACAAGAAAGTCGAGGCTC
  TGGATTCACTTCAGAGCAGAGCCAAACTGCTCAGGAATAAAGCTGGCTGGTTGGACAGCGAACTCAACAT
  GTTCACACACCAGTACCTGCAGCCCAGCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210494 representing NM_133201
  Red=Cloning site Green=Tags(s)
  
  MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESASFLEDTHRNTELDPVT
  TEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLLSGIGHTTNCFLRVGGTD
  GHEAFLLTEGSEEKKSVKTVNQLAHALHQDEQLHAGSMVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELD
  SWIDKFCLDADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMER
  CTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVRMFEFQNFERQFEECIS
  QSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLEMREERQDRLRFIDKQLELLAQDYKLRIK
  QITEEVERQVSTAMAEEIRRLSVLVDEYQMDFHPSPVVLKVYKNELHRHIEEGLGRNLSDRCSTAIASSL
  QTMQQDMIDGLKPLLPVSMRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPK
  NSRRALLGYSDQVQRPLPLTPANPSMPPLPQSSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVG
  WRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFAHLC
  QQVDITRDNLEQEIAAMNKKVEALDSLQSRAKLLRNKAGWLDSELNMFTHQYLQPSR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133201
• ORF Size: 2271 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133201.2, NP_573464.2
• RefSeq Size: 4377
• RefSeq ORF: 2274
• Locus ID: 170731