Ercc3 (NM_133658) Mouse Tagged ORF Clone

CAT#: MG210632

Ercc3 (GFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ercc3
• Synonyms: BTF2 p89; Ercc-3; XPB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210632 representing NM_133658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAAAGAGATCGAGTGGACCGCGACAAAAAGAAATCCAAGAAGAGGCAGTATGAAGAGGAAGAGG
  AAGACGAAGACGACATTCCTGGGAACGAGTCTCAGGAGGCGGTGCCCTCCGCCGCTGGGAAACAGGTGGA
  CGAGTCCAGCACCAAAGTGGATGAGTATGGAGCAAAGGACTACAGACAGCAGATGCCACTAAAGGGTGAC
  CATACCTCCAGGCCCCTCTGGGTGGCTCCTGATGGCCACATTTTCTTGGAAGCCTTCTCTCCAGTTTACA
  AATATGCCCAAGACTTCCTGGTGGCAATTGCAGAGCCGGTGTGCCGGCCTACTCATGTACATGAATACAA
  GCTAACCGCCTACTCCCTCTATGCAGCGGTCAGTGTTGGGCTGCAAACCAGTGACATTACTGAGTACCTC
  AGAAAGCTCAGTAAGACTGGAGTTCCTGATGGAATTATCCAGTTTATTAAGCTGTGCACTGTCAGCTATG
  GGAAGGTCAAGCTGGTCCTCAAGCACAACAGGTACTTTGTTGAAAGTTCCCACCCTGATGTTATCCAGCA
  TCTTCTTCAAGACCCAGTGATCCGGGAATGTCGCTTGAGGAACGCCGAGGGCGAGGCCACCGAACTCATC
  ACAGAGACTTTTACAAGCAAATCTGCTATTTCTAAGACGGCGGCGGAAGGCAGTGGCGGGCCTTCTACTT
  CGCAGGGGGTAGATGCACAGGCCACGTCTGACATACCCAAAGACCTGTTTGATTTTTATGAGCAAATGGA
  CAAGGATGAGGAGGAGGAGGAAGAGACACAGACAGTGTCCTTTGAAGTTAAGCAGGAAATGATCGAGGAG
  CTGCAGAAGCGCTGCATCTGCTTAGAGTACCCGCTGCTGGCAGAGTATGACTTCCGGAATGACACTCTCA
  ATCCTGACATCAACATTGACCTGAAGCCCACAGCCGTACTCAGACCCTATCAGGAGAAGAGCCTGCGGAA
  GATGTTTGGGAACGGACGAGCACGCTCAGGAGTCATTGTTCTTCCCTGTGGTGCTGGGAAGTCCCTGGTC
  GGCGTGACTGCCGCATGCACTGTCAGAAAGCGCTGTCTCGTCCTGGGCAACTCGGCTGTGTCTGTGGAGC
  AGTGGAAAGCCCAGTTTAAGATGTGGTCAACCATCGATGACAGCCAGATCTGCCGCTTCACCTCAGATGC
  CAAGGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACTTACTCTATGCTGGGCCACACCACCAAAAGG
  TCCTGGGAAGCTGAGAGAGTCATGGAGTGGCTCAAAACCCAGGAGTGGGGCCTCATGATCCTTGACGAGG
  TGCACACCATTCCAGCCAGGATGTTCCGGCGGGTTCTGACTATTGTGCAGGCGCACTGTAAGCTTGGTTT
  GACTGCAACCCTTGTCCGGGAAGATGACAAAATTGTTGACTTAAATTTCCTGATTGGGCCCAAGCTCTAT
  GAAGCCAACTGGATGGAGCTGCAGAACAATGGGTACATCGCTAAAGTCCAGTGTGCTGAGGTTTGGTGCC
  CGATGTCTCCCGAGTTCTACCGAGAGTATGTGGCAATCAAAACAAAGAAACGGATCCTGTTGTACACCAT
  GAATCCCAACAAATTCAGAGCCTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATT
  GTCTTTGCTGACAACGTATTTGCCTTGAAGGAATATGCCATTCGGCTGAACAAACCTTATATCTACGGGC
  CCACGTCCCAGGGAGAACGTATGCAGATTCTCCAGAACTTCAAGCACAACCCCAAAATCAACACCATCTT
  CATCTCCAAGGTTGGTGACACATCCTTTGATCTGCCAGAAGCCAATGTCCTCATTCAGATCTCTTCCCAT
  GGTGGCTCCCGACGGCAGGAAGCCCAGAGACTGGGACGGGTACTCAGAGCCAAGAAAGGGATGGTCGCAG
  AGGAATACAATGCCTTTTTCTACTCCCTGGTATCTCAGGACACACAGGAAATGGCTTATTCTACCAAGCG
  ACAGAGATTCCTAGTGGATCAAGGTTACAGCTTTAAGGTAATCACAAAGCTAGCTGGCATGGAGGAAGAG
  GAGCTGGCGTTCTCCACCAAAGAGGAGCAGCAGCAGCTCCTGCAGAAGGTGCTGGCAGCCACCGACTTGG
  ATGCAGAGGAGGAAGTGGTGGCTGGAGAGTTTGGCTCTAGATCTGGCCAGGCATCCCGACGCTGTGGCAC
  CATGAGCTCCCTGTCAGGCGCAGACGACACCGTGTACATGGAGTACCACTCCTCCCGAAGCAAGGCCTCC
  AGCAAGCACGTGCACCCGCTTTTCAAACGCTTCAGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210632 representing NM_133658
  Red=Cloning site Green=Tags(s)
  
  MGKRDRVDRDKKKSKKRQYEEEEEDEDDIPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGD
  HTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYL
  RKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELI
  TETFTSKSAISKTAAEGSGGPSTSQGVDAQATSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEE
  LQKRCICLEYPLLAEYDFRNDTLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLV
  GVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKR
  SWEAERVMEWLKTQEWGLMILDEVHTIPARMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLY
  EANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKII
  VFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSH
  GGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEE
  ELAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSGQASRRCGTMSSLSGADDTVYMEYHSSRSKAS
  SKHVHPLFKRFRK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133658
• ORF Size: 2349 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133658.1, NP_598419.1
• RefSeq Size: 2673
• RefSeq ORF: 2352
• Locus ID: 13872
• Gene Summary: ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. [UniProtKB/Swiss-Prot Function]