Sox6 (NM_001025560) Mouse Tagged ORF Clone

CAT#: MG210647

Sox6 (GFP-tagged) - Mouse SRY-box containing gene 6 (Sox6), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sox6
• Synonyms: AI987981; SOX-LZ
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210647 representing NM_001025560
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCAAGCAAGCCACCTCTCCATTTGCCTGTACAGCGGATGGAGAGGAAGCAATGACCCAGGACT
  TGACCTCAAGGGAAAAGGAAGAGGGCAGTGATCAACACCCGGCCTCCCATCTGCCTCTGCACCCCATAAT
  GCACAACAAACCTCACTCTGAGGAGCTGCCTACACTTGTCAGTACCATTCAACAAGATGCTGACTGGGAC
  AGCGTTCTGTCATCTCAGCAAAGAATGGAGTCAGAGAATAATAAGTTATGTTCCCTATATTCCTTCCGAA
  ATACGTCTACCTCACCACATAAGCCTGACGAAGGGAGTCGGGAGCGTGAAATAATGAACAGTGTTACTTT
  TGGAACCCCGGAACGCCGCAAAGGGAGCCTTGCCGATGTGGTGGATACGCTGAAGCAGAAGAAGCTGGAG
  GAGATGACTCGGACAGAACAAGAGGATTCCTCCTGCATGGAAAAACTACTTTCAAAAGATTGGAAGGAAA
  AAATGGAAAGACTAAATACGAGTGAACTTCTTGGAGAAATCAAAGGTACACCTGAGAGCCTTGCAGAGAA
  AGAACGGCAACTCTCCACCATGATTACCCAGCTGATCAGCTTGCGGGAGCAGCTCCTGGCAGCCCATGAT
  GAACAGAAAAAGCTGGCAGCATCGCAGATCGAGAAACAGCGGCAGCAAATGGACCTTGCTCGCCAACAGC
  AAGAACAGATCGCAAGACAACAGCAGCAACTTCTACAGCAGCAGCACAAGATTAATCTCCTGCAGCAACA
  GATCCAGCAGGTTCAGGGGCACATGCCTCCGCTCATGATCCCAATTTTTCCACATGACCAGCGGACCTTG
  GCAGCAGCTGCTGCTGCCCAACAGGGATTCCTCTTCCCCCCTGGAATAACATACAAGCCAGGTGATAACT
  ACCCCGTACAGTTCATTCCGTCAACAATGGCAGCTGCTGCTGCTTCTGGACTCAGCCCTTTACAGCTTCA
  GCAGCTCTATGCCGCTCAGCTGGCCAGCATGCAGGTGTCACCTGGAGCAAAGATGCCATCAACTCCACAG
  CCACCAAACTCGGCAGGGGCAGTCTCACCTACTGGGATAAAAAATGAAAAGAGAGGGACCAGCCCTGTAA
  CTCAAGTTAAGGATGAAACAACAGCCCAGCCGCTGAATCTCTCATCCCGGCCTAAGACAGCAGAGCCTGT
  GAAGTCCCCAACATCTCCCACCCAGAACCTCCTCCCAGCCAGCAAAACCAGCCCTGTCAACCTGCCAAAC
  AAAAGCAGCATCCCCAGCCCCATTGGAGGAAGCCTGGGGAGAGGATCCTCTCTAGATATACTATCCAGTC
  TCAACTCTCCTGCCCTGTTTGGGGACCAGGACACAGTGATGAAAGCCATTCAGGAGGCTCGGAAGATGCG
  AGAACAGATCCAGCGGGAGCAACAACAGCAGCCACACGGAGTTGATGGGAAACTGTCTTCCATGAACAAC
  ATAGGGCTGAGCAACTGCAGGACTGAGAAGGAAAGAACACGCTTTGAGAACCTGGGTCCCCAATTAACAG
  GAAAGTCAAGTGAAGATGGAAAACTGGGCCCGGGTGTCATCGATCTCACTCGGCCAGAGGACGCAGAAGG
  AAGCAAAGCAATGAATGGCTCTGCAGCTAAACTACAGCAGTATTATTGTTGGCCAACAGGAGGCGCCACT
  GTGGCTGAAGCCCGTGTCTACAGGGATGCCCGCGGCCGTGCCGGCAGCGAACCCCACATCAAGCGACCAA
  TGAATGCGTTCATGGTTTGGGCAAAGGACGAAAGGAGGAAAATTCTTCAGGCCTTCCCTGACATGCATAA
  CTCCAACATTAGCAAAATCTTAGGATCTCGCTGGAAATCCATGTCCAACCAGGAGAAGCAACCTTACTAT
  GAAGAACAGGCCCGGCTAAGCAAAATCCACCTAGAGAAGTACCCAAACTACAAGTACAAACCCCGGCCGA
  AGCGCACATGCATCGTGGATGGCAAGAAGCTCCGGATTGGGGAGTACAAGCAACTGATGCGCTCGCGAAG
  GCAGGAGATGCGACAGTTCTTCACTGTGGGGCAACAGCCTCAGATGCCCATCACCACAGGAACAGGTGTT
  GTGTATCCTGGTGCTATTACTATGGCAACGACCACACCATCACCTCAGATGACATCTGACTGCTCTAGCA
  CCTCTGCCAGCCCCGAGCCCAGCCTCCCTGTCATCCAGAGCACGTATGGTATGAAGATGGACGGCGCAAG
  CCTGGCTGGGAACGACATGATTAATGGAGAGGATGAAATGGAAGCCTATGACGACTACGAAGATGATCCC
  AAATCAGACTATAGCAGCGAGAATGAGGCCCCAGAGCCTGTCAGTGCCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210647 representing NM_001025560
  Red=Cloning site Green=Tags(s)
  
  MSSKQATSPFACTADGEEAMTQDLTSREKEEGSDQHPASHLPLHPIMHNKPHSEELPTLVSTIQQDADWD
  SVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSREREIMNSVTFGTPERRKGSLADVVDTLKQKKLE
  EMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGTPESLAEKERQLSTMITQLISLREQLLAAHD
  EQKKLAASQIEKQRQQMDLARQQQEQIARQQQQLLQQQHKINLLQQQIQQVQGHMPPLMIPIFPHDQRTL
  AAAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQQLYAAQLASMQVSPGAKMPSTPQ
  PPNSAGAVSPTGIKNEKRGTSPVTQVKDETTAQPLNLSSRPKTAEPVKSPTSPTQNLLPASKTSPVNLPN
  KSSIPSPIGGSLGRGSSLDILSSLNSPALFGDQDTVMKAIQEARKMREQIQREQQQQPHGVDGKLSSMNN
  IGLSNCRTEKERTRFENLGPQLTGKSSEDGKLGPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGAT
  VAEARVYRDARGRAGSEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYY
  EEQARLSKIHLEKYPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQMPITTGTGV
  VYPGAITMATTTPSPQMTSDCSSTSASPEPSLPVIQSTYGMKMDGASLAGNDMINGEDEMEAYDDYEDDP
  KSDYSSENEAPEPVSAN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025560
• ORF Size: 8678 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025560.1
• RefSeq Size: 8193 bp
• RefSeq ORF: 2358 bp
• Locus ID: 20679
• Cytogenetics: 7 61.29 cM
• Gene Summary: This gene encodes a member of a family of transcriptional regulators containing high mobility group (HMG) DNA-binding domains. Function of the encoded protein is important for proper cardiac and skeletal development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]