Cdh12 (NM_001008420) Mouse Tagged ORF Clone

CAT#: MG210697

Cdh12 (GFP-tagged) - Mouse cadherin 12 (Cdh12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdh12
• Synonyms: Cdhb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210697 representing NM_001008420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTACAAGGAACTCTTTATACCTGCTTCTCTGGATCCTGTTTGATGGAGGTCTCCTAACACCACTTC
  AGCCACAGCCCCAACAGACTTTAGCCACAGAACCAAAAGAAAATGTTATCCACCTTTCGGGGAGACGATC
  CCATTTCCAACGAGTTAAACGTGGCTGGGTATGGAATCAGTTTTTTGTGCTGGAAGAGTACATGGGCTCC
  GAACCTCAATATGTGGGGAAGCTGCATTCGGACTTGGATAAAGGAGAGGGCACTGTTAAATACACGCTCT
  CCGGAGATGGTGCTGGCACTGTTTTTACAATTGATGAAACTACAGGAGACATTCATGCAATAAGAAGCCT
  GGATAGAGAAGAAAAACCTTTCTACACTCTTCGTGCTCAGGCAGTGGACATAGAAACCAGGAAGCCACTG
  GAGCCTGAATCAGAGTTCATCATTAAAGTGCAGGATATTAATGACAATGAACCAAAGTTTTTGGATGGAC
  CTTATGTTGCTAGTGTTCCAGAAATGTCTCCTGTGGGTGCGTATGTGCTGCAGGTGAAGGCCACAGATGC
  AGACGATCCTACCTATGGGAACAGTGCCAGAGTTGTTTACAGCATCCTTCAGGGGCAACCTTATTTCTCT
  ATTGATCCCAAAACAGGTGTCATTAGAACAGCATTGCCAAACATGGACAGAGAAGTCAAAGAGCAGTACC
  AGGTCCTCATTCAAGCGAAAGACATGGGAGGACAGCTCGGAGGACTTGCTGGAACTACAGTTGTCAACAT
  CACCCTTACTGATGTCAATGACAACCCACCTCGCTTCCCAAAAAGCATCTTCCATCTGAAAGTTCCCGAG
  TCTTCCCCTGTTGGCTCAGCTATTGGAAGAATAAGAGCAGTAGATCCTGATTTTGGAAAAAATGCAGAAA
  TTGAATACAACATTGTCCCAGGAGATGGGGGAAATTTGTTTGACATTGTCACAGATGAGGATACACAAGA
  AGGAATCATCAAATTGAAAAAGCCTTTAGATTTTGAAACCAAGAAGGCATATACTTTTAAAGTAGAGGCC
  TCCAACCTTCACCTTGACCACCGCTTTCACTCTGCTGGGCCATTTAAGGATACTGCTACAGTAAAAATCA
  GCGTGCTGGATGTGGATGAGCCACCAGTGTTCAGCAAACCACTGTACACCATGGAGGTTTATGAAGACAC
  TCCTGTGGGGACCATCATCGGAGCTGTCACAGCACAAGACCTTGATGTGGGCAGTAGTGCTGTTAGGTAC
  TTCATAGATTGGAAGAGTGATGGGGAGAGCTACTTTACAATAGATGGAACGGAAGGAACCATCGCCACTA
  ATGAATTACTAGACAGAGAGAACATGGCGCAGTATAATTTCTCCATAATTGCAAGTAAAGTTAGTAACCC
  ATTATTAACTAGCAAAGTTAACATACTAATTAATGTCCTAGATGTCAATGAATTTCCTCCAGAAATATCT
  GTGCCATATGAGACAGCTGTATGTGAGAATGCCAAGCCAGGACAGATAATTCAGATAGTTGGTGCTGTAG
  ACCGAGATCTCTCACCTGCTGGGCAGCAATTCTCCTTTAGATTAGCACCAGAGGCTGTCATCAAACCAAA
  TTTTACAGTCCATGACTTCAGAAACAACACAGCTGGAATCGAAACCCGAAGAAATGGCTATAGCCGCAGG
  CAGCAGGAGTTGTATTTCCTTCCCATTGTAATAGAAGACAGCAGTTATCCTGTCCAGAGCAGCACGAATA
  CAATGACTATTCGTGTTTGTAGGTGTGACTCAGATGGGACCATCCTGTCTTGCAACGTGGAAGCAATTTT
  TCTACCTGTAGGACTCAGCACTGGAGCTTTGATTGCAATCCTACTATGCATGGTTATACTCTTAGCTATT
  GTTGTGCTGTATGTAGCACTGAGGAGGCAGAGGAAGAAAGACACCCTTATGACCTCTAAAGAAGACATCA
  GAGACAATGTCATCCATTATGACGATGAAGGAGGTGGGGAGGAGGACACCCAGGCCTTTGACATCGGGGC
  TCTGAGAAATCCCAAGGTGATCGAAGAGAACAAAATTCGCAGGGATATAAAACCAGACTCCCTCTGTTTA
  TCTCGTCAAAGACCACAAGTGGAAGACAACACAGACATAAGGGATTTTATTCACCGAAGGCTACAGGAAA
  ATGACGCAGACCCAGCGGCTCCACCATACGATTCCTTGGCCACTTATGCGTATGAGGGGAATGGCTCAGT
  GGCAGAATCACTCAGCTCCATAGACTCTCTCACCACAGAAGCCGACCAGGACTATGACTATCTATCAGAC
  TGGGGACCCCGCTTTAAGGTCTTGGCAGACATGTTTGGAGAAGAAGAGAGTTACAACCCCGACAATGTTA
  CC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210697 representing NM_001008420
  Red=Cloning site Green=Tags(s)
  
  MLTRNSLYLLLWILFDGGLLTPLQPQPQQTLATEPKENVIHLSGRRSHFQRVKRGWVWNQFFVLEEYMGS
  EPQYVGKLHSDLDKGEGTVKYTLSGDGAGTVFTIDETTGDIHAIRSLDREEKPFYTLRAQAVDIETRKPL
  EPESEFIIKVQDINDNEPKFLDGPYVASVPEMSPVGAYVLQVKATDADDPTYGNSARVVYSILQGQPYFS
  IDPKTGVIRTALPNMDREVKEQYQVLIQAKDMGGQLGGLAGTTVVNITLTDVNDNPPRFPKSIFHLKVPE
  SSPVGSAIGRIRAVDPDFGKNAEIEYNIVPGDGGNLFDIVTDEDTQEGIIKLKKPLDFETKKAYTFKVEA
  SNLHLDHRFHSAGPFKDTATVKISVLDVDEPPVFSKPLYTMEVYEDTPVGTIIGAVTAQDLDVGSSAVRY
  FIDWKSDGESYFTIDGTEGTIATNELLDRENMAQYNFSIIASKVSNPLLTSKVNILINVLDVNEFPPEIS
  VPYETAVCENAKPGQIIQIVGAVDRDLSPAGQQFSFRLAPEAVIKPNFTVHDFRNNTAGIETRRNGYSRR
  QQELYFLPIVIEDSSYPVQSSTNTMTIRVCRCDSDGTILSCNVEAIFLPVGLSTGALIAILLCMVILLAI
  VVLYVALRRQRKKDTLMTSKEDIRDNVIHYDDEGGGEEDTQAFDIGALRNPKVIEENKIRRDIKPDSLCL
  SRQRPQVEDNTDIRDFIHRRLQENDADPAAPPYDSLATYAYEGNGSVAESLSSIDSLTTEADQDYDYLSD
  WGPRFKVLADMFGEEESYNPDNVT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008420
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008420.2, NP_001008420.1
• RefSeq Size: 5666 bp
• RefSeq ORF: 2385 bp
• Locus ID: 215654
• Cytogenetics: 15 8.25 cM
• Gene Summary: This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. This gene is expressed in both the excitatory and inhibitory neurons in various areas of the adult central nervous system including the cerebellum, neocortex, hippocampus and in different subcortical nuclei. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 15. [provided by RefSeq, Nov 2015]