Grm4 (NM_001013385) Mouse Tagged ORF Clone

CAT#: MG210868

Grm4 (GFP-tagged) - Mouse glutamate receptor, metabotropic 4 (Grm4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grm4
• Synonyms: Gprc1d; mGluR4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210868 representing NM_001013385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGAAGGGAGGCTGGGCCTGGTGGTGGGCCCGGCTGCCCCTCTGCCTACTCCTCAGCCTTTATG
  GCTCCTGGGTGCCTTCATCCCTAGGAAAGCCCAAGGGTCACCCCCACATGAACTCTATCCGTATCGATGG
  AGACATCACCCTGGGAGGCCTGTTTCCCGTCCACGGTCGCGGCTCCGAGGGCAAGGCCTGCGGCGAGTTG
  AAGAAGGAGAAAGGTATCCACCGGCTGGAGGCCATGCTCTTTGCCCTGGACCGCATCAACAACGACCCGG
  ACCTACTGCCCAACATCACGTTGGGCGCCCGCATTCTGGACACCTGCTCAAGGGATACCCACGCCCTGGA
  GCAGTCCCTGACCTTTGTGCAGGCGCTCATCGAGAAGGACGGCACGGAGGTCCGCTGCGGCAGCGGGGGC
  CCACCCATCATCACCAAGCCTGAACGAGTGGTGGGTGTCATCGGAGCTTCGGGGAGCTCCGTCTCGATCA
  TGGTGGCCAACATCCTCCGCCTCTTCAAGATCCCCCAGATCAGCTACGCCTCCACGGCTCCCGACTTGAG
  TGATAACAGCCGCTATGACTTCTTCTCCCGGGTCGTGCCCTCGGACACATACCAGGCCCAGGCCATGGTG
  GACATCGTCCGGGCCCTCAAGTGGAACTATGTGTCCACGCTGGCCTCAGAGGGTAGCTACGGCGAGAGCG
  GCGTGGAGGCCTTTATCCAGAAGTCCCGAGAGAACGGAGGCGTGTGCATTGCCCAGTCGGTGAAGATTCC
  ACGGGAACCCAAGACCGGGGAGTTTGACAAGATCATCAAACGCCTTCTGGAAACGTCCAATGCCAGAGCC
  ATCATCATCTTTGCCAACGAGGATGATATCAGGAGGGTGCTGGAGGCAGCGCGCAGGGCCAACCAGACCG
  GCCACTTCTTTTGGATGGGTTCTGATAGCTGGGGCTCCAAGAGCGCCCCCGTGCTGCGCCTTGAGGAAGT
  GGCTGAAGGTGCAGTCACCATTCTTCCCAAGAGGACGTCTGTGCGAGGGTTTGACCGATACTTCTCCAGC
  CGCACGCTTGACAACAACAGGCGCAACATCTGGTTTGCTGAGTTCTGGGAGGACAACTTCCATTGCAAGT
  TGAGCCGCCACGCGCTCAAGAAGGGAAGCCACATCAAGAAGTGCACCAACCGAGAGCGCATCGGGCAGGA
  CTCGGCCTACGAACAGGAGGGGAAGGTGCAGTTTGTGATCGACGCCGTGTACGCCATGGGCCATGCTCTG
  CACGCCATGCATCGTGACCTGTGTCCCGGCCGCGTAGGACTCTGCCCTCGAATGGACCCTGTGGATGGCA
  CCCAGCTGCTTAAGTACATCAGAAACGTCAACTTCTCAGGCATCGCCGGGAACCCGGTGACCTTCAACGA
  GAACGGAGACGCGCCAGGGCGTTATGACATCTACCAGTACCAACGTCGCAACGGCTCGGCTGAGTACAAG
  GTCATCGGCTCATGGACAGACCACTTGCACCTCAGAATAGAGCGGATGCAGTGGCCAGGGAGTGGCCAGC
  AGCTGCCACGCTCCATCTGCAGCCTGCCCTGCCAGCCAGGCGAGCGGAAGAAGACGGTGAAGGGCATGGC
  TTGCTGCTGGCACTGCGAGCCCTGCACGGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACCTGC
  CCCTATGACATGCGGCCCACGGAGAACCGCACGAGCTGCCAGCCCATACCCATTGTCAAGTTGGAGTGGG
  ACTCACCCTGGGCTGTGCTGCCCCTCTTCCTGGCTGTGGTGGGCATTGCTGCCACGCTGTTCGTGGTGGT
  CACTTTTGTGCGCTACAACGACACTCCGATCGTCAAGGCCTCGGGCCGGGAGCTGAGCTACGTCCTGCTG
  GCGGGCATCTTTCTCTGCTATGCCACCACCTTCCTCATGATCGCAGAGCCTGACCTGGGGACCTGTTCAC
  TCCGCCGCATCTTCCTGGGGCTTGGCATGAGCATCAGCTACGCGGCCCTGCTGACCAAGACCAACCGCAT
  CTACCGCATCTTTGAGCAGGGCAAGCGGTCGGTCAGCGCCCCACGGTTCATCAGCCCCGCCTCGCAGCTG
  GCCATCACCTTCGTCCTCATCTCGCTGCAGTTGCTTGGCATCTGCGTGTGGTTCGTGGTGGACCCCTCCC
  ACTCGGTGGTGGACTTCCAGGACCAGCGGACACTTGACCCCCGCTTCGCCCGGGGTGTGCTCAAGTGTGA
  CATCTCGGACCTGTCGCTCATCTGCCTCCTGGGCTACAGCATGCTGCTGATGGTCACGTGTACTGTGTAT
  GCCATCAAGACTCGAGGCGTGCCTGAGACCTTCAATGAGGCCAAGCCCATCGGCTTCACCATGTACACCA
  CCTGCATCGTCTGGCTGGCCTTCATCCCCATCTTTTTTGGCACCTCGCAGTCGGCTGACAAGGTAACCTC
  TGAGGCCCTGCCCGTGGAATTCAGCCCGCCATTGCTGGCACATAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210868 representing NM_001013385
  Red=Cloning site Green=Tags(s)
  
  MSGKGGWAWWWARLPLCLLLSLYGSWVPSSLGKPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKACGEL
  KKEKGIHRLEAMLFALDRINNDPDLLPNITLGARILDTCSRDTHALEQSLTFVQALIEKDGTEVRCGSGG
  PPIITKPERVVGVIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSDTYQAQAMV
  DIVRALKWNYVSTLASEGSYGESGVEAFIQKSRENGGVCIAQSVKIPREPKTGEFDKIIKRLLETSNARA
  IIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKSAPVLRLEEVAEGAVTILPKRTSVRGFDRYFSS
  RTLDNNRRNIWFAEFWEDNFHCKLSRHALKKGSHIKKCTNRERIGQDSAYEQEGKVQFVIDAVYAMGHAL
  HAMHRDLCPGRVGLCPRMDPVDGTQLLKYIRNVNFSGIAGNPVTFNENGDAPGRYDIYQYQRRNGSAEYK
  VIGSWTDHLHLRIERMQWPGSGQQLPRSICSLPCQPGERKKTVKGMACCWHCEPCTGYQYQVDRYTCKTC
  PYDMRPTENRTSCQPIPIVKLEWDSPWAVLPLFLAVVGIAATLFVVVTFVRYNDTPIVKASGRELSYVLL
  AGIFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPRFISPASQL
  AITFVLISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARGVLKCDISDLSLICLLGYSMLLMVTCTVY
  AIKTRGVPETFNEAKPIGFTMYTTCIVWLAFIPIFFGTSQSADKVTSEALPVEFSPPLLAHN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013385
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013385.1, NP_001013403.1
• RefSeq Size: 3002 bp
• RefSeq ORF: 2499 bp
• Locus ID: 268934
• Cytogenetics: 17 A3.3