Nedd4l (NM_031881) Mouse Tagged ORF Clone

CAT#: MG210959

Nedd4l (GFP-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 4-like (Nedd4l)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nedd4l
• Synonyms: 1300012C07Rik; Nedd4-2; Nedd4b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210959 representing NM_031881
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGACCCTATACATTTAAGGATTTTCTCCTGCGACCTAGAAGTCATAAATCTCGAGTCAAGGGGT
  TTTTGAGGTTGAAAATGGCCTACATGCCGAAAAACGGAGGTCAGGATGAAGAAAACAGCGAGCAGAGGGA
  TGACATGGAGCATGGATGGGAAGTGGTTGACTCCAACGACTCAGCTTCCCAGCACCAGGAGGAGCTCCCC
  CCTCCTCCCCTGCCGCCAGGATGGGAAGAGAAAGTGGACAATTTAGGCCGAACTTACTATGTCAACCACA
  ACAACAGGAGTACTCAGTGGCACCGACCCAGCTTGATGGATGTGTCCTCGGAATCAGACAATAACATCAG
  GCAGATCAACCAGGAGGCTGCACACCGACGCTTCCGCTCTCGGAGGCACATTAGTGAAGATTTGGAGCCT
  GAGGCCTCTGAAGGCGGTGGAGAAGGCCCTGAGCCTTGGGAGACCATTTCAGAGGAAATGAACATGGCAG
  GAGATTCTCTCAGCCTGGCTCTGCCCCCACCGCCTGCCTCCCCAGTGTCCCGGACCAGCCCCCAGGAGCT
  GTCGGAAGAAGTGAGCCGAAGGTTGCAGATCACTCCGGACTCCAACGGGGAACAGTTCAGTTCTCTGATT
  CAGAGAGAGCCCTCGTCAAGGCTTCGGTCCTGCAGTGTTACCGACACGGTTGCTGAGCAAGCTCACCTTC
  CACCGCCCAGCACCCCAACTAGGCGAGCCCGTTCGTCAACTGTCACGGGTGGTGAGGAACCCACGCCATC
  AGTGGCCTATGTACATACCACGCCGGGCCTGCCTTCAGGCTGGGAAGAAAGAAAGGATGCAAAGGGACGC
  ACATACTATGTCAATCATAACAATCGAACCACAACTTGGACTCGGCCAATCATGCAGCTTGCAGAAGACG
  GTGCCTCCGGATCAGCCACAAACAGTAACAACCACCTAGTCGAACCCCAGATCCGCCGGCCCCGTAGCCT
  CAGCTCGCCAACAGTAACTTTATCTGCCCCACTGGAGGGTGCCAAGGATTCACCCATCCGCCGTGCCGTG
  AAAGATACCCTTTCCAATCCACAGTCCCCTCAGCCATCACCTTACAACTCCCCCAAACCACAACACAAAG
  TCACACAGAGCTTCCTGCCACCAGGCTGGGAGATGAGGATAGCCCCCAACGGCCGACCCTTCTTCATTGA
  CCATAACACAAAGACTACAACCTGGGAAGATCCACGGTTGAAATTTCCAGTACACATGCGGTCAAAAGCA
  TCTTTAAACCCCAATGACCTGGGCCCTCTTCCTCCTGGCTGGGAAGAGAGGATCCACTTGGATGGCCGCA
  CGTTTTACATTGACCATAATAGTAAAATTACCCAGTGGGAAGATCCAAGACTACAGAACCCAGCCATCAC
  TGGTCCGGCTGTTCCGTACTCCAGAGAGTTTAAGCAGAAATACGACTACTTTAGGAAGAAATTAAAGAAG
  CCTGCTGATATTCCAAACAGGTTTGAAATGAAACTTCACAGAAACAACATATTTGAAGAGTCCTATCGGA
  GGATCATGTCTGTAAAGAGACCTGACGTCCTAAAGGCTAGGCTGTGGATTGAGTTTGAATCAGAGAAAGG
  CCTGGACTATGGGGGCGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTTAACCCCTACTAT
  GGCCTCTTCGAGTACTCTGCCACGGACAACTACACACTTCAGATCAATCCCAACTCAGGCCTCTGTAATG
  AAGACCATTTGTCCTATTTCACCTTCATTGGAAGAGTTGCTGGCCTAGCGGTGTTTCATGGGAAACTCTT
  AGATGGATTCTTCATTCGACCATTCTACAAGATGATGCTGGGGAAGCAGATAACGCTGAACGACATGGAG
  TCCGTGGACAGCGAGTACTACAACTCTTTGAAGTGGATCTTAGAAAACGACCCCACGGAACTTGACCTCA
  TGTTCTGCATAGACGAAGAGAACTTTGGGCAGACATACCAAGTGGATCTGAAGCCCAACGGGTCAGAAAT
  AATGGTAACCAATGAGAACAAACGAGAATACATTGACTTAGTCATCCAGTGGAGATTTGTGAACAGGGTC
  CAGAAGCAAATGAATGCCTTCTTGGAGGGATTTACAGAACTTCTTCCAATCGACTTGATTAAAATTTTTG
  ATGAAAATGAGCTGGAGTTGCTGATGTGCGGCCTTGGTGATGTCGACGTGAACGACTGGAGACAGCACTC
  TATTTACAAGAACGGCTACTGCCCCAACCACCCTGTCATCCAGTGGTTCTGGAAGGCCGTGCTCCTGATG
  GATGCTGAGAAGCGCATCCGGTTACTACAGTTTGTCACAGGCACCTCCAGAGTACCCATGAATGGATTTG
  CCGAACTCTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCCGAAAAACTACC
  CAGAGCTCATACATGCTTTAATCGCCTTGATTTACCTCCATATGAAACCTTTGAAGATTTACGGGAGAAA
  CTTCTCATGGCTGTGGAAAACGCTCAAGGCTTCGAAGGTGTGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210959 representing NM_031881
  Red=Cloning site Green=Tags(s)
  
  MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDDMEHGWEVVDSNDSASQHQEELP
  PPPLPPGWEEKVDNLGRTYYVNHNNRSTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEP
  EASEGGGEGPEPWETISEEMNMAGDSLSLALPPPPASPVSRTSPQELSEEVSRRLQITPDSNGEQFSSLI
  QREPSSRLRSCSVTDTVAEQAHLPPPSTPTRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGR
  TYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLVEPQIRRPRSLSSPTVTLSAPLEGAKDSPIRRAV
  KDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKA
  SLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKK
  PADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYY
  GLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDME
  SVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRV
  QKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLM
  DAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREK
  LLMAVENAQGFEGVD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031881
• ORF Size: 2565 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031881.1, NP_114087.1
• RefSeq Size: 3326
• RefSeq ORF: 2568
• Locus ID: 83814