Usp5 (NM_013700) Mouse Tagged ORF Clone

CAT#: MG210971

Usp5 (GFP-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Usp5
• Synonyms: AA407472; ISOT; ISOT-1; Ucht
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210971 representing NM_013700
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAGTGAAGAGGCGCTGCTGTCAGTGTTACCGACGATCCGTGTCCCCAAGGCGGGAGACC
  GGGTCCATAAAGACGAGTGCGCTTTCTCTTTCGACACGCCGGAGTCTGAGGGTGGCCTCTATATCTGCAT
  GAACACATTCCTGGGATTCGGGAAGCAGTATGTGGAGAGACACTTCAACAAGACAGGCCAGCGTGTCTAC
  CTGCACCTCCGGAGGACCCGGCGACCGAAAGAAGAGGACACCAGTGCAGGCACTGGAGACCCACCTCGGA
  AGAAGCCCACCCGGCTGGCCATTGGTGTTGAAGGAGGGTTTGACCTCACCGAGGACAAGTTTGAATTTGA
  CGAGGATGTGAAGATTGTCATTTTGCCCGATTACCTGGAGATCGCTCGGGATGGGTTGGGGGGGCTTCCT
  GACATTGTCAGAGATCGGGTGACCAGTGCTGTGGAAGCCCTACTTTCTGCTGACTCAGCCTCTCGAAAGC
  AGGAGGTCCAGGCTTGGGATGGGGAAGTCCGTCAGGTGTCCAAGCATGCCTTCAACCTCAAGCAGTTGGA
  CAACCCTGCTCGGATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGCGACATGCGCGAGAACCTGTGGCTC
  AACCTGACAGACGGCTCCATCCTCTGTGGACGGCGCTACTTCGATGGCAGTGGGGGCAACAACCATGCTG
  TGGAGCACTACAGGGAGACTGGCTACCCCTTAGCTGTTAAGCTGGGCACCATCACCCCGGATGGAGCTGA
  CGTGTATTCTTACGACGAGGATGACATGGTTCTGGACCCCAGCCTGGCAGAGCACTTGTCTCACTTTGGC
  ATCGACATGCTGAAGATGCAGAAGACGGACAAGACGATGACTGAGCTGGAGATAGACATGAACCAGCGGA
  TTGGTGAATGGGAGTTGATCCAGGAGTCAGGCGTGCCACTCAAGCCTCTGTTTGGGCCTGGTTACACGGG
  CATCCGGAACCTGGGTAACAGCTGCTATCTCAACTCCGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTT
  CAGAGGAAGTATGTGGATAAATTGGAGAAGATCTTCCAGAATGCCCCAACGGACCCCACCCAAGACTTCA
  GCACCCAGGTGGCCAAGCTGGGGCATGGCCTTCTCTCTGGAGAATACTCTAAGCCAGCACTGGAGTCAGG
  TGATGGGGAGCAGGTACCAGAACAAAAGGAAGTTCAAGATGGCATTGCCCCTCGAATGTTCAAGGCCCTC
  ATTGGCAAGGGCCACCCTGAGTTCTCCACTAATCGGCAGCAGGATGCCCAGGAATTCTTCCTACATCTTA
  TCAACATGGTGGAGAGGAATTGCCGGAGTTCTGAAAATCCAAATGAAGTGTTCCGCTTTTTGGTGGAAGA
  AAAGATCAAATGCCTGGCCACAGAGAAGGTTAAGTACACTCAGCGAGTTGACTACATCATGCAGTTGCCT
  GTACCCATGGATGCAGCCCTTAACAAAGAGGAGCTTTTAGAGTATGAAGAGAAGAAGCGGCAAGCTGAAG
  AGGAGAAAGTGCCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGCTCCTGCCTGGAAGCCTACGG
  GGCCCCTGAGCAGGTGGACGACTTCTGGAGTACAGCTCTACAGGCGAAGTCGGTCGCTGTCAAGACCACG
  CGCTTTGCCTCCTTCCCCGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTTGGCTTAGACTGGGTGC
  CCAAGAAGCTGGATGTGTCCATTGAGATGCCAGAGGAGCTCGATATCTCCCAGCTGAGGGGCACAGGGCT
  ACAGCCGGGAGAGGAGGAGCTGCCTGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGGTAGC
  CTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCCCCTCACTTCTCCTCTCCGACATCACCCA
  TGCTGGACGAATCCGTCATCATACAGTTGGTGGAAATGGGCTTCCCTATGGATGCCTGCCGGAAAGCTGT
  GTACTATACGGGAAACAGCGGGGCCGAAGCAGCCATGAACTGGGTCATGTCACACATGGATGATCCAGAT
  TTTGCAAACCCCCTCATCCTGCCTGGCTCCAGTGGGCCTGGCTCCACAAGTGCAGCAGCTGACCCCCCGC
  CGGAGGACTGTGTGACCACCATAGTCTCCATGGGCTTCTCCAGGGACCAGGCCCTGAAAGCTCTGCGGGC
  CACGAACAATAGCTTAGAGCGGGCTGTAGACTGGATCTTCAGCCACATTGATGACCTGGACGCAGAAGCT
  GCTATGGACATCTCAGAGGGACGCTCAGCGGCTGAGTCCATCTCCGAGTCTGTGCCAGTGGGACCTAAAG
  TCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATGGGCACATCTACTATGTGTGG
  TCACTATGTCTGCCATATCAAGAAGGAAGGCAGGTGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCT
  GAAAAGCCACCCAAGGACCTGGGCTACATCTACTTCTACCAGAGAGTGGTCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210971 representing NM_013700
  Red=Cloning site Green=Tags(s)
  
  MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVY
  LHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLP
  DIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWL
  NLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFG
  IDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF
  QRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKAL
  IGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLP
  VPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTT
  RFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGS
  LGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPD
  FANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEA
  AMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCAS
  EKPPKDLGYIYFYQRVVS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013700
• ORF Size: 2574 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013700.1, NP_038728.1
• RefSeq Size: 3176
• RefSeq ORF: 2577
• Locus ID: 22225