Pde6a (NM_146086) Mouse Tagged ORF Clone

CAT#: MG210979

Pde6a (GFP-tagged) - Mouse phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde6a
• Synonyms: nmf282; Pdea
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210979 representing NM_146086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAGGTGACAGCAGAGGAGGTAGAAAAGTTCCTGGATTCAAATATTGGCTTTGCCAAACAGTACT
  ATAACTTTCACTACCGGGGGAAGGTCATCTCAGACCTCCTCGGGGCCAAGGAGGCAGCCGTGGACTTCAG
  CAACTACCACGATGTGAACAGCGTAGAGGAGAGTGAGATCATCTTTGACCTCCTGCGGGACGTTCAGGAG
  AACTTACAGGCTGAGAAATGCACATTCAATGTCATGAAGAAGCTCTGCTTCCTCCTGCGGGCTGACCGCA
  TGAGCCTGTTCATGTACAGGACCCGCAACGGGATCGCCGAGTTGGCCACTAGGCTCTTCAATGTCCACAA
  GGATGCCGTCCTAGAGGACTGCTTGGTGATGCCCGACTCCGAGATTGTCTTCCCTCTGGACATGGGTGTC
  GTGGGCCACGTCGCACACTCCAAAAAGATTGCCAATGTCCCCAACACAGAAGAGGATGAGCATTTCTGTG
  ACTTCGTGGACAATCTCACAGAATATCAGACCAAAAACATCCTGGCTTCCCCCATCATGAATGGGAAGGA
  TGTGGTAGCCATAATCATGGCTGTGAATAAAATAGATGAACCCCACTTCACCAAGAGAGATGAAGAGATT
  CTTCTCAAGTACCTCAACTTTGTGAACCTGATCATGAAGGTATTCCACCTGAGCTACCTGCACAACTGTG
  AGACTCGTCGCGGCCAGATATTGCTGTGGTCTGGGAGCAAGGTCTTTGAGGAGCTCACGGATATAGAGAG
  GCAGTTCCACAAGGCCCTGTACACGGTCCGGGCTTTCCTCAACTGTGACAGATACTCCGTAGGACTCTTA
  GACATGACCAAACAGAAGGAATTTTTTGATGTGTGGCCAGTTCTGATGGGCGAGGCTCCAGCTTACTCTG
  GTCCCAGGACTCCAGACGGAAGGGAAATTAACTTCTACAAGGTCATTGACTACATCCTGCACGGCAAAGA
  AGACATCAAAGTCATCCCGAACCCACCCGCTGACCACTGGGCTCTAGTGAGTGGTCTCCCCACTTACGTG
  GCTCAAAATGGTCTGATCTGCAATATAATGAATGCGCCTGCAGAGGACTTTTTTGAATTCCAGAAAGAGC
  CTCTGGATGAGTCTGGGTGGATGATTAAAAATGTACTCTCCATGCCCATCGTCAACAAGAAGGAAGAGAT
  CGTCGGCGTGGCCACATTTTACAACCGCAAAGATGGGAAGCCCTTCGACGATATGGACGAGACCCTCATG
  GAGTCTTTGACTCAGTTTCTGGGATGGTCAGTCTTAAACCCTGACACCTACGAGTCCATGAACAAGCTCG
  AGAACAGGAAGGATATCTTCCAGGACATCGTGAAATATCACGTGAAGTGTGATAACGAAGAAATCCAGAA
  GATCTTGAAAACCAGAGAGGTGTACGGCAAAGAGCCGTGGGAATGCGAGGAGGAGGAGCTGGCTGAGATC
  CTGCAAGGAGAACTTCCAGACGCGGAGTCATACGAAATCAACAAGTTCCACTTCAGCGACCTGCCACTCA
  CGGAGCTGGAGCTGGTGAAGTGCGGCATCCAGATGTACTACGAGCTCAGAGTGGTGGACAAGTTCCACAT
  CCCGCAAGAGGCCCTGGTGCGCTTCATGTATTCGCTAAGCAAAGGCTACCGGAGAATCACTTACCACAAC
  TGGCGGCATGGCTTCAACGTGGGGCAGACCATGTTCTCCTTGCTGGTGACAGGAAAGCTGAAACGGTACT
  TCACTGATCTAGAGGCCTTGGCCATGGTCACTGCTGCCTTCTGTCATGACATCGACCACAGAGGCACGAA
  CAACCTCTACCAGATGAAATCACAGAACCCCCTGGCCAAGCTCCATGGGTCCTCCATCTTGGAAAGGCAT
  CATTTGGAGTTTGGCAAAACACTCCTGAGAGATGAGAGCCTGAATATCTTCCAGAACCTGAATCGCCGGC
  AGCATGAGCACGCGATCCACATGATGGACATCGCGATCATTGCCACAGACCTTGCCTTGTATTTCAAGAA
  AAGGACCATGTTCCAGAAGATTGTGGATCAGTCAAAGACATATGAGAGTACCCAGGAGTGGACCCAGTAC
  ATGATGCTGGAGCAGACACGGAAGGAAATTGTGATGGCCATGATGATGACCGCCTGTGATCTCTCAGCCA
  TCACCAAACCCTGGGAGGTACAGAGCAAGGTGGCTCTGCTGGTGGCTGCTGAATTCTGGGAGCAAGGTGA
  CCTGGAGCGCACAGTGCTGCAGCAGAATCCCATTCCCATGATGGACAGAAACAAGGCGGATGAGCTCCCC
  AAGCTTCAAGTCGGCTTCATCGACTTTGTGTGCACTTTTGTCTATAAGGAGTTCTCCCGATTTCATGAGG
  AGATTACACCCATGCTGGATGGGATCACTAACAACCGCAAGGAATGGAAGGCGCTGGCTGATGAGTACGA
  AGCCAAGATGAAGGCCCTGGAGGAGGAGAAGCAGAAGCAGCAGGCAGCCAAGCAAGCTGCTTCCGGGAAC
  CAGCCAGGAGGGAACCCCACTCCAGGGGGTGCACCTGCATCTAAGTCCTGTTGCATCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210979 representing NM_146086
  Red=Cloning site Green=Tags(s)
  
  MGEVTAEEVEKFLDSNIGFAKQYYNFHYRGKVISDLLGAKEAAVDFSNYHDVNSVEESEIIFDLLRDVQE
  NLQAEKCTFNVMKKLCFLLRADRMSLFMYRTRNGIAELATRLFNVHKDAVLEDCLVMPDSEIVFPLDMGV
  VGHVAHSKKIANVPNTEEDEHFCDFVDNLTEYQTKNILASPIMNGKDVVAIIMAVNKIDEPHFTKRDEEI
  LLKYLNFVNLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLL
  DMTKQKEFFDVWPVLMGEAPAYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYV
  AQNGLICNIMNAPAEDFFEFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDDMDETLM
  ESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEI
  LQGELPDAESYEINKFHFSDLPLTELELVKCGIQMYYELRVVDKFHIPQEALVRFMYSLSKGYRRITYHN
  WRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERH
  HLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESTQEWTQY
  MMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELP
  KLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYEAKMKALEEEKQKQQAAKQAASGN
  QPGGNPTPGGAPASKSCCIQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146086
• ORF Size: 2580 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_146086.2, NP_666198.1
• RefSeq Size: 4230
• RefSeq ORF: 2583
• Locus ID: 225600