Enpp2 (NM_015744) Mouse Tagged ORF Clone

CAT#: MG210991

Enpp2 (GFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Enpp2
• Synonyms: ATX; E-NPP 2; lysoPLD; Npps2; PD-Ialpha; Pdnp2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210991 representing NM_015744
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGACAAGGCTGTTTCGGGTCATACCAGGTAATATCCTTGTTCACTTTTGCCATCGGCGTCAATC
  TCTGCTTAGGATTCACAGCAAGTCGAATTAAGAGGGCCGAATGGGATGAAGGACCTCCCACAGTGTTATC
  TGACTCTCCATGGACCAACACATCTGGATCCTGCAAAGGTAGATGCTTTGAGCTTCAAGAGGTTGGACCT
  CCTGACTGTCGGTGTGACAACCTATGTAAGAGCTACAGCAGCTGCTGCCATGATTTTGATGAGCTCTGTT
  TGAAAACAGCTCGAGGCTGGGAGTGCACCAAAGACAGATGTGGGGAAGTACGAAATGAGGAAAATGCCTG
  TCACTGCTCAGAAGACTGCTTGTCCCGGGGAGACTGCTGTACCAACTATCAAGTGGTCTGCAAAGGAGAA
  TCACACTGGGTAGATGATGACTGTGAAGAAATAAGAGTCCCTGAATGCCCTGCAGGGTTTGTCCGCCCTC
  CGTTAATCATCTTCTCTGTGGATGGATTCCGTGCATCGTACATGAAGAAAGGCAGCAAGGTTATGCCCAA
  CATTGAGAAACTGCGGTCCTGTGGCACCCATGCTCCCTACATGAGGCCTGTGTACCCTACAAAAACCTTC
  CCTAATCTGTATACGCTGGCCACTGGTTTATATCCAGAATCCCATGGAATCGTTGGCAATTCAATGTATG
  ACCCTGTCTTTGATGCTACTTTCCATCTTCGAGGGCGAGAGAAGTTTAACCATAGATGGTGGGGAGGCCA
  ACCGCTATGGATTACAGCCACCAAGCAAGGGGTGAGAGCCGGGACATTCTTTTGGTCTGTGAGCATCCCT
  CACGAGCGGAGAATCCTAACTATCCTTCAGTGGCTTTCCCTGCCAGACAATGAGAGGCCTTCAGTTTATG
  CCTTCTACTCCGAGCAGCCTGATTTTTCTGGACACAAGTACGGCCCTTTTGGCCCTGAGATGACAAATCC
  TCTGAGGGAGATTGACAAGACCGTGGGGCAGTTAATGGACGGACTGAAACAACTCAAGCTGCACCGTTGT
  GTGAATGTTATCTTTGTTGGAGACCATGGAATGGAAGACGTGACATGTGACAGAACTGAGTTCTTGAGCA
  ACTATCTGACTAACGTGGATGATATTACTTTAGTACCTGGAACTCTAGGAAGAATTCGACCCAAGATTCC
  CAATAATCTTAAATATGACCCTAAAGCCATTATTGCTAACCTCACGTGTAAAAAACCAGATCAGCACTTT
  AAGCCTTACATGAAACAGCACCTTCCCAAACGTTTGCACTATGCCAACAATCGGAGAATCGAGGATCTCC
  ATTTATTGGTGGAACGCAGATGGCATGTTGCAAGGAAACCTTTGGACGTTTATAAGAAGCCGTCAGGAAA
  ATGTTTTTTCCAGGGTGACCACGGCTTTGATAACAAGGTCAATAGCATGCAGACTGTTTTTGTAGGTTAT
  GGCCCAACTTTTAAGTACAGGACTAAAGTGCCTCCATTTGAAAACATTGAACTTTATAATGTTATGTGCG
  ATCTCCTAGGCTTGAAGCCAGCTCCCAATAATGGAACACATGGAAGTTTGAATCACCTGCTACGCACAAA
  TACCTTTAGGCCAACCCTACCAGAGGAAGTCAGCAGACCCAATTACCCAGGGATTATGTACCTTCAGTCT
  GATTTTGACCTGGGCTGCACCTGTGATGATAAGGTAGAGCCAAAGAACAAATTGGAAGAACTAAATAAAC
  GCCTTCATACCAAAGGATCTACAGAAGAGAGACATCTCCTGTATGGACGACCTGCAGTGCTTTATCGGAC
  TAGCTATGATATCTTATACCATACGGACTTTGAAAGTGGTTACAGTGAAATATTCTTAATGCCTCTCTGG
  ACTTCTTATACCATTTCTAAGCAGGCTGAGGTCTCTAGCATCCCAGAGCACCTGACCAACTGTGTTCGCC
  CTGATGTCCGTGTATCTCCTGGATTCAGTCAGAACTGTTTAGCCTATAAAAATGATAAACAGATGTCCTA
  TGGATTCCTTTTTCCTCCCTATCTGAGCTCTTCCCCAGAAGCGAAATATGATGCATTCCTTGTAACCAAC
  ATGGTTCCAATGTACCCTGCCTTCAAACGTGTTTGGACTTATTTCCAAAGGGTCTTGGTGAAGAAATATG
  CGTCAGAAAGGAATGGGGTCAACGTAATAAGTGGACCGATCTTTGACTACAATTACGATGGCTTACGTGA
  CATTGAGGATGAAATTAAACAGTATGTGGAAGGCAGCTCTATTCCTGTCCCTACCCACTACTACAGCATC
  ATCACCAGCTGCCTGGACTTCACTCAGCCTGCAGACAAGTGTGATGGTCCTCTCTCTGTGTCTTCTTTCA
  TCCTTCCTCACCGACCTGACAATGATGAGAGCTGTAATAGTTCCGAGGATGAGTCGAAGTGGGTAGAGGA
  ACTCATGAAGATGCACACAGCTCGGGTGAGGGACATCGAGCATCTCACCGGTCTGGATTTCTACCGGAAG
  ACTAGCCGTAGCTATTCGGAAATTCTGACCCTCAAGACATACCTGCATACATATGAGAGCGAGATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210991 representing NM_015744
  Red=Cloning site Green=Tags(s)
  
  MARQGCFGSYQVISLFTFAIGVNLCLGFTASRIKRAEWDEGPPTVLSDSPWTNTSGSCKGRCFELQEVGP
  PDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGE
  SHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTF
  PNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIP
  HERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRC
  VNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHF
  KPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGY
  GPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQS
  DFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLW
  TSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTN
  MVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYDGLRDIEDEIKQYVEGSSIPVPTHYYSI
  ITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRK
  TSRSYSEILTLKTYLHTYESEI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015744
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015744.1, NP_056559.1
• RefSeq Size: 3242
• RefSeq ORF: 2589
• Locus ID: 18606
• Gene Summary: This gene encodes a member of the phosphodiesterase and nucleotide pyrophosphatase family of bifunctional enzymes that hydrolize phosphodiester bonds of various nucleotides. The encoded protein undergoes proteolytic processing to generate a mature protein with lysophospholipase D activity, catalyzing the cleavage of the choline group from lysophosphatidylcholine to produce lysophosphatidic acid. This gene is expressed in numerous tissues and participates in neural development, obesity, inflammation and oncogenesis. A complete lack of the encoded protein in mice results in aberrant vascular and neuronal development leading to embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015]