Aco1 (NM_007386) Mouse Tagged ORF Clone

CAT#: MG211074

Aco1 (GFP-tagged) - Mouse aconitase 1 (Aco1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aco1
• Synonyms: Aco-1; Irebp; Irp1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211074 representing NM_007386
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACCCATTTGCACACCTTGCTGAGCCCTTGGACGCTGCCCAACCAGGAAAGAGGTTCTTCAACT
  TGAATAAATTGGAGGACTCAAGATACGGACGCTTACCATTTTCTATCAGAGTTCTCCTGGAGGCAGCCGT
  TCGGAACTGTGATGAGTTTCTGGTGAAGAAAAATGACATCGAGAATATCCTGAATTGGAATGTCATGCAA
  CATAAGAACATAGAAGTGCCGTTTAAGCCAGCCCGAGTCATACTGCAAGACTTTACGGGTGTGCCTGCTG
  TGGTGGATTTTGCAGCAATGCGCGATGCTGTGAAGAAGTTGGGAGGGAATCCGGAGAAAATTAACCCTGT
  CTGCCCCGCTGACCTTGTAATTGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAG
  AAGAATCAAGACCTGGAGTTTGAAAGAAATAAAGAACGATTTGAATTTCTAAAGTGGGGTTCCCAGGCCT
  TTTGCAACATGCGGATAATCCCTCCTGGCTCGGGAATCATTCACCAAGTGAACCTGGAGTATTTGGCAAG
  AGTAGTGTTTGATCAGGATGGATGCTACTACCCAGACAGCCTCGTGGGCACAGATTCACACACGACCATG
  ATTGACGGCCTGGGAGTTCTTGGTTGGGGTGTGGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGC
  CCATCAGCATGGTGCTTCCCCAGGTGATTGGCTACAAGCTGATGGGGAAGCCTCACCCTCTGGTAACATC
  CACGGACATCGTGCTCACCATTACCAAGCACCTCCGACAAGTGGGAGTTGTGGGCAAATTTGTGGAGTTT
  TTCGGGCCAGGAGTGGCTCAGCTGTCCATTGCTGACCGAGCTACGATTGCCAATATGTGCCCAGAGTATG
  GTGCGACAGCAGCCTTCTTCCCGGTTGATGAAGTTAGCATCGCGTACCTGCTGCAGACAGGCCGCGAGGA
  AGACAAAGTCAAGCACATTCAGAAGTATCTTCAGGCTGTAGGCATGTTTCGAGATTTCAACGACACCTCT
  CAAGACCCAGACTTCACTCAGGTTGTGGAGTTAGATCTGAAAACAGTTGTGCCTTGCTGCAGTGGACCCA
  AAAGACCTCAGGACAAAGTCGCAGTGTCTGAGATGAAAAAGGACTTTGAAAGCTGCCTTGGAGCCAAGCA
  AGGATTTAAAGGTTTCCAAGTTGCTTCAGACCGTCACAATGACCGCAAGACGTTCCTCTATAATAACAGT
  GAATTCACTCTCGCTCACGGCTCTGTGGTAATCGCTGCCATCACTAGCTGCACAAACACCAGCAATCCAT
  CCGTGATGTTAGGAGCAGGATTGTTAGCAAAGAAAGCTGTGGAGGCGGGGCTGAGTGTGAAGCCTTACAT
  CAAAACCAGCCTGTCTCCTGGAAGTGGAGTGGTCACCTACTACCTTCGAGAGAGTGGAGTCATGCCTTAC
  CTGTCCCAGTTAGGGTTTGATGTGGTGGGCTACGGCTGCATGACCTGCATCGGCAACAGTGGACCCCTTC
  CTGAACCTGTGGTCGAGGCGATCACCCAGGGAGACCTCGTAGCTGTTGGCGTTCTGTCTGGGAATAGGAA
  TTTTGAAGGCCGAGTCCATCCTAACACACGAGCCAACTACTTAGCATCCCCCCCTCTGGTAATAGCATAT
  GCGATTGCAGGCACCGTCAGGATTGACTTCGAGAAAGAGCCTTTGGGAGTGAACGCACAGGGCCGGCAAG
  TGTTTCTGAAGGATATCTGGCCCACGCGAGATGAGATCCAGGCGGTGGAAAGGCAGTATGTCATCCCCGG
  GATGTTTAAGGAGGTCTATCAGAAAATAGAGACTGTAAATAAAAGCTGGAATGCCTTAGCAGCCCCGTCA
  GAGAAGCTGTACGCGTGGAATCCCAAATCTACTTACATTAAGTCGCCGCCATTCTTTGAAAGCTTGACTT
  TAGATCTCCAGCCACCCAAGTCCATAGTGGATGCCTATGTGCTACTGAATTTAGGAGATTCAGTAACAAC
  GGACCATATCTCTCCCGCCGGCAATATTGCAAGAAACAGCCCTGCGGCTCGCTACTTGACGAACAGAGGC
  CTAACACCACGAGAGTTCAACTCCTATGGCTCCCGCCGGGGTAACGACGCCATCATGGCCCGGGGGACAT
  TTGCCAACATTCGCTTGCTGAACAAGTTTCTGAACAAGCAGGCACCTCAGACTGTCCATCTCCCTTCAGG
  AGAAACCCTTGATGTATTCGACGCTGCTGAGCGGTACCAGCAGGCTGGACTTCCCCTGATTGTTCTGGCT
  GGGAAAGAATACGGTTCAGGCAGCTCCCGAGACTGGGCAGCCAAAGGCCCTTTCCTGCTGGGAATCAAAG
  CTGTCCTGGCAGAGAGCTATGAGCGCATTCACCGCAGCAACTTGGTTGGCATGGGGGTGATCCCCCTTGA
  GTATCTCCCTGGTGAAACTGCAGACTCTCTGGGACTCACAGGTCGGGAAAGATACACTATCAACATCCCT
  GAAGACCTCAAGCCTCGCATGACGGTGCAGATCAAGCTGGACACTGGGAAGACCTTCCAGGCCGTGATGA
  GGTTCGACACTGATGTGGAGCTCACATACTTCCACAATGGAGGCATCCTGAACTACATGATCCGAAAGAT
  GGCCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211074 representing NM_007386
  Red=Cloning site Green=Tags(s)
  
  MKNPFAHLAEPLDAAQPGKRFFNLNKLEDSRYGRLPFSIRVLLEAAVRNCDEFLVKKNDIENILNWNVMQ
  HKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPVCPADLVIDHSIQVDFNRRADSLQ
  KNQDLEFERNKERFEFLKWGSQAFCNMRIIPPGSGIIHQVNLEYLARVVFDQDGCYYPDSLVGTDSHTTM
  IDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYKLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEF
  FGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIAYLLQTGREEDKVKHIQKYLQAVGMFRDFNDTS
  QDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSEMKKDFESCLGAKQGFKGFQVASDRHNDRKTFLYNNS
  EFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPY
  LSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAY
  AIAGTVRIDFEKEPLGVNAQGRQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNKSWNALAAPS
  EKLYAWNPKSTYIKSPPFFESLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRG
  LTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTVHLPSGETLDVFDAAERYQQAGLPLIVLA
  GKEYGSGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGETADSLGLTGRERYTINIP
  EDLKPRMTVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMAQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007386
• ORF Size: 2667 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007386.2, NP_031412.2
• RefSeq Size: 4319
• RefSeq ORF: 2670
• Locus ID: 11428
• Gene Summary: This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism. Disruption of this gene is associated with pulmonary hypertension and polycythemia. [provided by RefSeq, Jan 2014]