Gria4 (NM_019691) Mouse Tagged ORF Clone

CAT#: MG211114

Gria4 (GFP-tagged) - Mouse glutamate receptor, ionotropic, AMPA4 (alpha 4) (Gria4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gria4
• Synonyms: GluA4; Glur-4; GluR-D; Glur4; Gluralpha4; spkw1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211114 representing NM_019691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATTATTTGCAGGCAGATTGTCTTGTTGTTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTC
  CGAGCAGCGTTCAAATAGGTGGTCTCTTTATCCGAAACACAGACCAGGAATACACTGCTTTTCGGCTAGC
  TATCTTTCTTCATAACACCAGCCCCAATGCATCTGAAGCCCCTTTCAATTTGGTACCTCATGTGGACAAC
  ATTGAGACTGCCAACAGTTTTGCTGTGACAAATGCATTCTGTTCCCAGTATTCTAGAGGGGTGTTTGCCA
  TTTTTGGACTCTATGACAAGAGGTCAGTGCATACCTTGACCTCCTTCTGCAGTGCTCTGCACATCTCTCT
  CATCACACCAAGCTTCCCCACTGAAGGAGAGAGCCAGTTCGTGCTTCAGCTAAGACCTTCATTGAGAGGT
  GCACTCCTGAGCCTCCTGGATCACTATGAATGGAATTGTTTTGTCTTCCTGTATGATACAGACAGGGGGT
  ATTCAATACTTCAAGCTATAATGGAAAAAGCAGGACAGAATGGATGGCATGTCAGTGCGATATGTGTGGA
  AAATTTTAACGATGTCAGCTACAGGCAACTACTAGAAGAGCTTGACAGAAGACAAGAGAAGAAATTTGTA
  ATAGATTGTGAGATAGAAAGGCTTCAAAACATATTAGAACAAATTGTGAGTGTTGGGAAGCACGTCAAAG
  GCTACCATTATATCATCGCAAATTTGGGTTTCAAAGATATTTCTCTTGAGAGATTTATACATGGAGGAGC
  AAATGTCACTGGATTCCAGTTAGTAGATTTTAATACGCCCATGGTGACGAAACTAATGGATCGCTGGAAG
  AAACTAGATCAACGAGAATATCCAGGATCTGAAACACCTCCAAAGTACACTTCTGCTCTCACTTACGATG
  GTGTCTTGGTAATGGCTGAAACTTTCCGAAGTCTCAGAAGACAGAAAATTGATATTTCAAGGAGAGGAAA
  TGCCGGGGATTGTCTGGCAAACCCTGCTGCTCCCTGGGGCCAGGGAATTGACATGGAGAGAACACTGAAG
  CAGGTTCGAATTCAAGGACTGACTGGGAATGTTCAATTTGACCACTATGGACGTAGAGTTAATTACACAA
  TGGATGTGTTTGAATTAAAAAGCACAGGACCTCGAAAGGTTGGCTATTGGAACGATATGGATAAATTAGT
  CTTGATTCAAGATGCGCCTACTCTTGGCAATGACACAGCAGCTATCGAGAACAGAACAGTGGTTGTAACC
  ACAATTATGGAATCCCCCTACGTTATGTACAAGAAAAATCATGAAATGTTTGAAGGAAATGACAAGTATG
  AAGGCTACTGTGTAGACTTGGCATCTGAAATTGCGAAACATATCGGTATCAAATATAAAATTGCCATTGT
  CCCTGATGGAAAATATGGAGCAAGGGATGCGGACACCAAAATTTGGAATGGGATGGTAGGAGAGCTTGTG
  TATGGGAAAGCAGAGATTGCCATTGCACCTCTGACAATCACGTTGGTGCGAGAGGAGGTCATCGACTTTT
  CTAAGCCTTTTATGAGTTTAGGCATCTCTATCATGATCAAAAAACCTCAGAAATCTAAACCAGGAGTGTT
  TTCCTTCTTGGACCCTCTGGCCTATGAGATCTGGATGTGCATAGTGTTTGCATACATTGGTGTCAGCGTG
  GTCTTGTTCCTAGTCAGTAGGTTTAGCCCATATGAGTGGCACACAGAAGAGCCTGAGGATGGAAAAGAAG
  GACCCAGTGACCAACCTCCCAATGAGTTTGGCATCTTTAACAGCCTCTGGTTTTCCCTGGGTGCCTTTAT
  GCAACAAGGATGTGACATTTCACCCAGATCCCTGTCCGGTCGGATTGTTGGAGGCGTATGGTGGTTCTTC
  ACTCTCATCATTATCTCATCCTACACTGCTAATCTGGCTGCATTCCTGACAGTGGAGAGAATGGTCTCCC
  CCATAGAAAGTGCAGAAGACCTGGCCAAACAAACAGAAATTGCCTATGGAACACTTGATTCGGGATCAAC
  AAAAGAATTCTTCAGAAGATCAAAAATAGCAGTATATGAAAAGATGTGGACCTACATGCGATCGGCAGAG
  CCATCTGTGTTCACTAGAACTACAGCTGAGGGCGTGGCCCGTGTCCGCAAGTCCAAGGGCAAATTTGCCT
  TCCTCCTGGAGTCCACGATGAATGAATACATTGAGCAGCGAAAGCCCTGTGACACGATGAAAGTGGGAGG
  AAACCTGGATTCCAAAGGCTATGGTGTAGCGACGCCCAAGGGTTCCTCATTAGGAACTCCTGTAAACCTT
  GCCGTTTTGAAACTCAGTGAGGCAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTG
  AATGTGGACCCAAGGACTCGGGAAGCAAGGACAAGACGAGTGCCTTGAGCCTGAGCAACGTAGCAGGCGT
  CTTCTACATTCTGGTTGGCGGCTTGGGCTTGGCAATGCTGGTGGCTTTGATAGAGTTCTGTTACAAGTCC
  AGGGCAGAGGCGAAGAGAATGAAGCTGACTTTTTCCGAAGCCATAAGAAACAAAGCCAGGTTATCCATCA
  CTGGGAGTGTGGGAGAAAACGGCCGTGTGCTGACCCCCGACTGCCCCAAGGCCGTACACACAGGAACTGC
  GATTAGACAGAGCTCGGGATTGGCTGTCATTGCATCGGACCTACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211114 representing NM_019691
  Red=Cloning site Green=Tags(s)
  
  MRIICRQIVLLFSGFWGLAMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFLHNTSPNASEAPFNLVPHVDN
  IETANSFAVTNAFCSQYSRGVFAIFGLYDKRSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRG
  ALLSLLDHYEWNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFV
  IDCEIERLQNILEQIVSVGKHVKGYHYIIANLGFKDISLERFIHGGANVTGFQLVDFNTPMVTKLMDRWK
  KLDQREYPGSETPPKYTSALTYDGVLVMAETFRSLRRQKIDISRRGNAGDCLANPAAPWGQGIDMERTLK
  QVRIQGLTGNVQFDHYGRRVNYTMDVFELKSTGPRKVGYWNDMDKLVLIQDAPTLGNDTAAIENRTVVVT
  TIMESPYVMYKKNHEMFEGNDKYEGYCVDLASEIAKHIGIKYKIAIVPDGKYGARDADTKIWNGMVGELV
  YGKAEIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSV
  VLFLVSRFSPYEWHTEEPEDGKEGPSDQPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFF
  TLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWTYMRSAE
  PSVFTRTTAEGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSSLGTPVNL
  AVLKLSEAGVLDKLKNKWWYDKGECGPKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKS
  RAEAKRMKLTFSEAIRNKARLSITGSVGENGRVLTPDCPKAVHTGTAIRQSSGLAVIASDLP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019691
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019691.4, NP_062665.3
• RefSeq Size: 5458
• RefSeq ORF: 2709
• Locus ID: 14802
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]