Grm1 (BC067057) Mouse Tagged ORF Clone

CAT#: MG211136

Grm1 (GFP-tagged) - Mouse glutamate receptor, metabotropic 1 (cDNA clone MGC:91369 IMAGE:30544252)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grm1
• Synonyms: mGluR1, 4930455H15Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211136 representing BC067057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCGGCTCCTCTTGATTTTCTTCCCAATGATCTTTTTGGAGATGTCCATTTTGCCCAGGATGCCTG
  ACAGAAAAGTACTGCTGGCAGGTGCCTCGTCCCAGCGCTCTGTGGCGAGAATGGACGGAGATGTCATCAT
  CGGAGCCCTCTTCTCAGTCCATCACCAGCCTCCAGCCGAAAAGGTACCGGAAAGGAAGTGTGGGGAGATC
  AGAGAACAGTATGGTATCCAGAGAGTGGAGGCCATGTTCCACACATTAGATAAGATTAATGCGGACCCGG
  TGCTCCTGCCCAACATCACTCTGGGCAGTGAGATCCGGGACTCCTGCTGGCACTCTTCTGTGGCTCTGGA
  ACAGAGCATTGAGTTCATCAGAGACTCCCTGATTTCCATCCGAGATGAGAAGGATGGACTGAACCGCTGC
  CTGCCTGATGGCCAGACCCTACCCCCAGGCAGGACTAAGAAGCCTATTGCTGGAGTGATCGGCCCTGGCT
  CCAGCTCTGTGGCCATTCAAGTCCAGAATCTTCTGCAGCTGTTCGACATCCCACAAATCGCCTATTCTGC
  CACGAGCATAGACCTGAGCGACAAAACTTTGTACAAATACTTCCTGAGGGTGGTCCCTTCTGACACTTTG
  CAGGCAAGGGCGATGCTTGATATCGTCAAGCGGTACAACTGGACCTATGTCTCTGCAGTCCACACAGAAG
  GGAATTACGGCGAGAGTGGAATGGATGCTTTCAAAGAGTTGGCTGCCCAGGAAGGACTCTGCATCGCACA
  CTCGGACAAAATCTACAGCAATGCTGGCGAGAAGAGCTTTGATCGGCTTCTGCGCAAGCTCCGAGAGCGG
  CTTCCCAAGGCCAGGGTTGTGGTCTGCTTCTGCGAGGGCATGACAGTGCGGGGGTTACTGAGTGCCATGC
  GCCGCCTTGGCGTCGTGGGAGAGTTCTCACTCATTGGAAGTGATGGATGGGCAGACAGAGATGAAGTCAT
  CGAAGGCTATGAGGTGGAAGCCAATGGGGGAATCACAATAAAGCTGCAGTCTCCAGAGGTCAGGTCGTTT
  GATGACTACTTTCTGAAGCTGAGGCTGGACACCAACACGAGGAATCCTTGGTTCCCTGAGTTCTGGCAAC
  ATCGCTTCCAGTGTCGCCTACCTGGACACCTCTTGGAAAACCCCAACTTTAAAAAAGTCTGCACAGGAAA
  TGAAAGCTTGGAAGAAAATTATGTCCAGGACAGCAAAATGGGATTTGTCATCAACGCCATCTATGCCATG
  GCACACGGCCTACAGAACATGCACCATGCTCTATGTCCTGGCTACGTGGGCCTTTGTGATGCCATGAAGC
  CCATTGACGGCAGGAAGCTCCTGGATTTCCTCATCAAATCCTCTTTTGTTGGAGTGTCTGGAGAGGAGGT
  CTGGTTCGATGAGAAGGGGGATGCACCTGGAAGGTATGACATTATGAATCTGCAGTACACAGAGGCTAAT
  CGCTATGACTATGTCCATGTGGGAACCTGGCATGAAGGTGTGCTGAATATCGATGATTACAAAATCCAGA
  TGAACAAAAGCGGAATGGTACGATCTGTGTGCAGCGAGCCTTGCTTAAAGGGTCAGATTAAGGTCATACG
  GAAAGGGGAAGTGAGCTGCTGCTGGATCTGCACAGCCTGCAAAGAGAATGAGTTTGTGCAAGATGAGTTT
  ACCTGCAGAGCCTGTGACCTGGGGTGGTGGCCCAATGCAGAGCTCACAGGCTGTGAGCCCATTACTATCC
  GTTACCTCGAGTGGAGTGACATAGAATCCATCATAGCCATCGCCTTTTCTTGCCTGGGCATCCTCGTGAC
  GCTATTTGTCACCCTCATCTTTGTGCTGTACCGGGACACACCTGTGGTCAAATCCTCCAGTAGAGAGCTC
  TGCTATATCATTCTGGCTGGTATTTTCCTCGGCTACGTATGCCCTTTCACCCTCATCGCCAAACCTACTA
  CCACATCCTGCTACCTCCAGCGCCTCCTAGTTGGCCTCTCTTCTGCCATGTGCTACTCTGCTCTTGTGAC
  CAAAACCAATCGTATTGCACGCATCCTGGCTGGCAGCAAGAAGAAGATCTGTACCCGGAAGCCCAGGTTC
  ATGAGCGCTTGGGCCCAAGTGATCATAGCCTCCATTCTGATTAGTGTACAGCTGACACTAGTGGTGACCT
  TGATCATCATGGAGCCTCCCATGCCCATTTTGTCCTACCCGAGCATCAAGGAAGTCTATCTTATCTGCAA
  TACCAGCAACCTGGGTGTAGTGGCACCTGTGGGTTACAACGGACTTCTCATCATGAGCTGTACCTACTAT
  GCCTTCAAGACCCGCAACGTGCCGGCCAATTTCAATGAGGCTAAATACATCGCCTTCACTATGTACACCA
  CCTGCATCATCTGGCTGGCTTTTGTTCCCATTTACTTTGGGAGCAACTACAAGATTATCACTACCTGCTT
  CGCAGTGAGCCTCAGTGTGACGGTGGCCCTGGGCTGCATGTTCACTCCCAAGATGTACATCATTATTGCT
  AAACCCGAGAGGAATGTCCGCAGTGCCTTCACCACCTCTGATGTAGTGCGCATGCACGTCGGTGACGGCA
  AGCTGCCATGCCGCTCCAACACCTTCCTCAACATTTTCCGGAGAAAGAAGCCTGGGGCAGGGAACGCCAA
  GAAGAGGCAGCCAGAATTCTCGCCCAGCAGCCAGTGTCCGTCGGCACATGTGCAGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211136 representing BC067057
  Red=Cloning site Green=Tags(s)
  
  MVRLLLIFFPMIFLEMSILPRMPDRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEI
  REQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGLNRC
  LPDGQTLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTL
  QARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRER
  LPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSF
  DDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKKVCTGNESLEENYVQDSKMGFVINAIYAM
  AHGLQNMHHALCPGYVGLCDAMKPIDGRKLLDFLIKSSFVGVSGEEVWFDEKGDAPGRYDIMNLQYTEAN
  RYDYVHVGTWHEGVLNIDDYKIQMNKSGMVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEFVQDEF
  TCRACDLGWWPNAELTGCEPITIRYLEWSDIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSREL
  CYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRF
  MSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPVGYNGLLIMSCTYY
  AFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIA
  KPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKPGAGNAKKRQPEFSPSSQCPSAHVQL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC067057
• ORF Size: 2720 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC067057, AAH67057
• RefSeq Size: 4437
• RefSeq ORF: 2720
• Locus ID: 14816
• Gene Summary: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum (By. similarity). [UniProtKB/Swiss-Prot Function]