Top3a (NM_009410) Mouse Tagged ORF Clone

CAT#: MG211428

Top3a (GFP-tagged) - Mouse topoisomerase (DNA) III alpha (Top3a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Top3a
• Synonyms: MGC106383
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211428 representing NM_009410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTTCCCGGTCACCCTCTTAGCGTTTCAGTGGCACCGACGGCCTGGAGGCCGTGCCCTGTCCCGCG
  CTGCCATGGAAGTGGCCTTCCGAGGAGTGCGAAAAGTTCTCTGCGTGGCGGAGAAAAACGACGCTGCCAA
  GGGGATCGCCGACTTGCTGTCCAACGGGCGTATGAGGCGGAAAGAAGGCCTTTCTAAATTCAACAAGATT
  TATGAATTTGACTATCATCTGTATGGCCAGAATGTTACTATGATAATGACTTCAGTCTCTGGACATTTGC
  TGGCTCATGACTTCCAGATGCAGTTTCGGAAATGGCAGAGCTGCAATCCCCTTGTCCTCTTTGAAGCAGA
  AATTGAAAAGTATTGCCCAGAAAATTTTATAGACATCAAGAAAACTCTAGAACGAGAGACACATCATTGT
  CAGGCCCTGGTGATCTGGACGGACTGTGATAGGGAAGGTGAGAACATCGGCTTTGAGATTATCCACGTAT
  GCAAGGCTGTAAAACCCAATCTACGGGTGCTGAGAGCCCGCTTCTCTGAGATCACGCCACACGCCGTCAG
  AACAGCCTGTGAAAACCTAACTGAGCCTGACCAGAGAGTGAGTGACGCAGTGGATGTGAGGCAAGAGCTG
  GACCTGAGGATCGGAGCTGCCTTCACAAGGTTCCAGACCCTGCGGCTCCAGAGGATCTTCCCTGAGGTGC
  TGGCAGAGCAGCTCATCAGCTACGGCAGTTGCCAGTTCCCAACACTGGGGTTTGTGGTGGAGAGGTTCAA
  GGCCATTCAGGCTTTTGTGCCAGAAGTCTTCCACAAAATTAAAGTAACTCATGACCACAAAGATGGGACC
  GTAGAGTTCAACTGGAAACGATACCGTCTCTTTAACCACACAGCTTGTCTTGTCCTTTATCAGTTGTGCA
  TGGAGGATCCCATGGCGACTGTGGTAGAGGTCAGATCTAAGCCAAAGAGCAAGTGGAGGCCGCAGGCTTT
  GGACACGGTGGAGCTAGAGAAACTGGCATCTCGGAAATTGAGAATAAATGCCAAAGAAACCATGAGGATC
  GCGGAAAAGCTCTATACACAAGGGTACATCAGCTACCCCCGGACAGAAACAAACATCTTCCCCAAAGACT
  TAAACCTGGTTGCATTGGTAGAACAGCAGACCGTGGATCCACACTGGGGGGCTTTTGCTCAGACCATTCT
  AGAGCGAGGTGGCCCTACTCCCCGAAACGGGAGCAAGTCTGATCAAGCTCACCCTCCCATTCACCCCACC
  AAATACACCAGCGGCTTGCAGGGAGATGATCGGCGACTGTACGAGTTCATTGTTCGCCATTTCCTGGCTT
  GCTGCTCTCAGGACGCTCAGGGGCAAGAGACTACCGTAGAGATCGACATTGCCCAAGAGCGCTTTGTGGC
  CCACGGGCTCATCATTCTGGCCCGTAACTACCTAGATGTGTATCCGTATGATCACTGGAGTGACAAGCTC
  CTCCCCGTCTATGAACAAGGCTCCCACTTCCAGCCCAGCACTGTGGAAATGGTGGATGGAGAGACTAGCC
  CACCCCAACTGCTTACCGAAGCTGACCTCATCGCCCTCATGGAGAAACATGGGATCGGTACTGATGCAAC
  TCATGCAGAACACATTGAGACCATCAAAGCCCGGATGTATGTGGGACTCACCTCAGACAAGCGGTTCCTG
  CCGGGGCACCTGGGCATGGGACTTGTAGAAGGTTACGATTCCATGGGTTATGAGATGTCCAAGCCTGACC
  TCCGTGCCGAGCTGGAAGCTGATCTCAAGCTGATCTGTGAGGGCAAGAAGGATAAGTTTCAAGTTCTAAG
  GCAGCAAGTGCAAAAATACAAGCAGGTTTTCATTGAAGCAGTGGCGAAGGCAAAGAAGTTGGATGAGGCT
  TTATCTCAGTACTTAGGAGAAAGAACAGAGATGGCCCAGCAAGAAGAGATCTACCCAGCCATGCCAGAGC
  CTGTTCGGAAGTGCCCACAGTGTAACAAGGATATGGTCCTCAAGACCAAGAAGAGTGGTGGGTTCTACCT
  CAGCTGCATGGGGTTCCCAGAGTGTCGGTCAGCTGTGTGGTTTCCTGACTCGGTGCTGGAGGCCAGCAGG
  GACAACAGTGTGTGTTCTGTTTGTCAGCCACCCCCTGTGTACAGGTTGAAGTTGAAGTTTAAGCGTGGTA
  GCCTTCCCCCAGCTATGCCCCTGGAGTTTGTCGGCTGCATAGGTGGATGTGATGAGACTTTGAAGGAAAT
  CTTCGGCCTGCGATTTCCACGGGCTCTCCCAAGAGCTAGCCAGCCCTCTGGCCACCTGCAGGCCAGCCAG
  GCCCTCAACAGGATGGACAGCAGTCAGCATAACCTGTCTCAACCTCTGGTTAACAGACATACTAGACCTT
  CAAAGACAGTGGCCCAAGCCTTGCTACCACCCACTACTGCTGGTGAAAGCAACTCTGTGACTTGCAATTG
  TGGCCGGGAGGCTGTGCTGCTCACTGTCCGCAAGCAGGGCCCCAACCAGGGCCGGCATTTCTACAAGTGC
  AGTAATGGTGACTGCAACTTCTTCCTGTGGGCTGACAGCAGCCATTCCACTGGAGGAGGGACCCCCACCT
  CTGCATCAGGACCCCCAGGCAGCTCTGTAGGATGCCCATCCAGTGTAGGCAGCCACATGGATGGGTTTGG
  CAGCCTTGGCAGCGACAGTGATGGAGGTACACCCTGCCTGTGCGGGCAGCCTGCTGTCACACGGACTGTT
  CAGAAGGATGGACCCAACAAAGGACGCCAGTTCCACACCTGTGCCAAGCCACGAGAGCAGCAGTGTGGCT
  TCTTTCAGTGGGTGGATGAGAATGTGGCCCCAGGAAGTTTTGCAGCCCCTGCATGGCCAGGAGGCAGAGG
  AAAAGCCCAGAGACCAGAGGCTGCAAGCAAAAGACCAAGAGCTGGTTCCTCAGATGCAGGGTCCACAGTA
  AAAAAGCCTCGGAAATGTAGCCTTTGTCACCAGCCTGGACACACCCGAACCTTTTGTCCTCAGAACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211428 representing NM_009410
  Red=Cloning site Green=Tags(s)
  
  MIFPVTLLAFQWHRRPGGRALSRAAMEVAFRGVRKVLCVAEKNDAAKGIADLLSNGRMRRKEGLSKFNKI
  YEFDYHLYGQNVTMIMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFIDIKKTLERETHHC
  QALVIWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQEL
  DLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEVFHKIKVTHDHKDGT
  VEFNWKRYRLFNHTACLVLYQLCMEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRI
  AEKLYTQGYISYPRTETNIFPKDLNLVALVEQQTVDPHWGAFAQTILERGGPTPRNGSKSDQAHPPIHPT
  KYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLIILARNYLDVYPYDHWSDKL
  LPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSDKRFL
  PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEA
  LSQYLGERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECRSAVWFPDSVLEASR
  DNSVCSVCQPPPVYRLKLKFKRGSLPPAMPLEFVGCIGGCDETLKEIFGLRFPRALPRASQPSGHLQASQ
  ALNRMDSSQHNLSQPLVNRHTRPSKTVAQALLPPTTAGESNSVTCNCGREAVLLTVRKQGPNQGRHFYKC
  SNGDCNFFLWADSSHSTGGGTPTSASGPPGSSVGCPSSVGSHMDGFGSLGSDSDGGTPCLCGQPAVTRTV
  QKDGPNKGRQFHTCAKPREQQCGFFQWVDENVAPGSFAAPAWPGGRGKAQRPEAASKRPRAGSSDAGSTV
  KKPRKCSLCHQPGHTRTFCPQNR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009410
• ORF Size: 3009 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009410.2, NP_033436.1
• RefSeq Size: 3740
• RefSeq ORF: 3012
• Locus ID: 21975