Iars2 (BC052403) Mouse Tagged ORF Clone

CAT#: MG211440

Iars2 (GFP-tagged) - Mouse isoleucine-tRNA synthetase 2, mitochondrial (cDNA clone MGC:63429 IMAGE:5715723)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Iars2
• Synonyms: MGC63429
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211440 representing BC052403
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTGGGGGCTGTGCCCTCGCGGGCCCGGGGCGGCGGCGGTCGCCGCGGCTGGGAGCTTCTGGGGGC
  CAGCCCGTTTGCCCAGCCGCCTAGGGTGTCTGGGGATGACGAGGAGGCTCGTGGTACGATCCGTGGCCGG
  GGCGGACAGCCCCCAGAGCAGCTCGAAAGGCGGCAGATACCGTGACACGGTGCTGCTGCCGCAGACCAGC
  TTCCCGATGAAGCTGCTGGGCCGCCAGCAGTCCGACATGGAGCTGGAGATCCAGCAGAAATGTGGCTTTT
  CAGAGCTTTATTCCTGGCAAAGAGAAAGAAAAGTAAAGACGGAATTTTGCCTTCATGACGGGCCTCCTTA
  TGCCAACGGTGACCCTCATGTTGGGCATGCCCTGAACAAGATTTTGAAAGACATTGCCAATCGATTCCAT
  ATGATGAGAGGTTCCAAGGTACACTTTGTGCCTGGCTGGGATTGCCATGGCTTGCCCATTGAAACAAAAG
  TGTTATCAGAGCTCGGTGTAGATGCCCAGAGTCTTTCAGCCATGGAGATCAGGGAGAAAGCCAGATCATT
  TGCTCAGGCAGCCATTGAAAAGCAGAAATCGGCGTTTGTCCGCTGGGGAGTGATGGCAGACTGGAATAAC
  TGCTATTACACATTTGACCCAAAGTACGAAGCCAAGCAGCTGAGAGTATTCTACCAAATGTATGAGAAGG
  GCTTGGTTTATCGATCTTACAAACCAGTATATTGGTCTCCCTCATCAAGGACTGCGTTGGCTGAAGCAGA
  GCTTGAATACAACCCTGAGCATGTCAGTCGGTCGATATACGTAAGATTCCCCCTCCTGAGACCTCCTCCG
  AAGTTGGAGTCTCTTACAGATGCCTCATCCCCTGTTAGCGTTTTAGTTTGGACCACCCAGCCTTGGACAA
  TTCCCGCCAACCAGGCAATTTGCTATATGCCAGAGGCAAAGTATGCTGTGGTGAAATGTTCTGCCTCTGG
  GCACCTCTACATCCTGGCTGAAGATAAAATAGCACCTGTCGCTTCTGCCTTGGAAACAACATTTGATGTT
  GTTGCAGCATTTTCAGGTGTGGATCTGGAAGGTGGCACTTGTAGTCACCCTTTAACTCCCGATAAAGTGT
  CTCCTCTTTTACCTGCTACCCACGTGACTATGGCCAAAGGAACAGGGTTGGTTCACACAGCCCCAGCTCA
  CGGTATGGAAGATTACAGCGTAGCATCTCAGCACAGCCTCCCTATGGACTGTTTAGTGGATGAAGGCGGA
  ATGTTCACCGATGCCGCAGGCCCTGAACTTCAGAACAAGGCTGTACTTAAAGAAGGAACAGATGTGGTTA
  TAAAGATGCTTCAAGCTACCAAGAATGTGTTGAAAGAAGAGAACATTGTGCACAGCTATCCCTGTGACTG
  GAGAACTAAGACACCCGTGCTGATTCGAGCCAGCAAGCAGTGGTTTGTAAATATCACGGACATCAAGGCT
  GCAGCTAAGGAGTCGTTGAAGACGGTGAAGTTCATTCCCGGAGCCGCACTGAACAGCATGACGGACATGC
  TGGACAGACGGCCGTACTGGTGCATATCCCGGCAGAGAGTCTGGGGGGTTCCCATTCCTGTGTTTCATCA
  TAAGACAAAAGATGAATATTTGATCAACAGCCAAACCACTGAGCATATTATTAAACTCGTCGAGCAGCAC
  GGCAGTGATGTGTGGTGGACCCTCCCTGCTGAGCAGCTTCTCCCGGCAGAAGTCCTAGCTCAGGCTGGTG
  GCCCTGGGGCTTTGGAATACGCGCCAGGGCAGGACATTTTGGACATCTGGTTTGACAGTGGAACTTCATG
  GTCTTGTGTTCTTCAAGACACGCAGCAGAGGGCAGACTTGTACCTGGAGGGAAAGGACCAGCTCGGGGGC
  TGGTTCCAGTCTTCCTTACTAACAAGTGTGGCCACCAGGTCAAAAGCACCTTTCAGGACAGTGATGGTTC
  ATGGATTTACACTTGGAGAAAAAGGAGAAAAGATGTCCAAGTCCCTCGGGAATGTCATTAATCCTGACAC
  TATCATCAGTGGAGGAAAAGACCACAGCAAAGAACCTCCCTACGGAGCTGACATCCTGCGCTGGTGGATA
  GCTGAGTCCAATGTCTTCACGGAGGTGACCATTGGCCCCTCTGTACTCTCTGCTGCCAGAGATGACATCA
  GCAAGCTCAGAAACACACTCCGCTTTCTCTTGGGAAATTTGACCGGTTTTAACCCCGAGACAGATTCTGT
  TCCCGTGAAGAATATGTATGTCATAGACCAGTATATGCTGCACTTAATTCAGGATTTTGCGACAAAGATT
  ACTGACTCATACAAACAGTATGACTTTGGGAAAGTTGTCAGGCTGTTGAAAGCCTTCTACACCAGAGAAC
  TGTCCAGCTTTTACTTCAGCATAGTCAAGGATAGGCTCTACTGCGAAAACGAGAAGGATCCCAAGCGCCG
  CTCGTGTCAGACAGCATTGGCAGAAATTTTGGATGTGTTGGTCCGTGCTTTTGCCCCCATTCTTCCTCAC
  TTGGCAGAAGAGGTGTTTCAGCACATTCCCTATGTTACAGAACCCAAGAGTGTTTTTCGCACTGGCTGGA
  TTAATACAAGCTCTATTTGGAAGAAGCCAGGACTGGAGGAGGCAGTGGAAAGTGCGTGCGCCATGAGGGA
  CTCCTTTCTTGGCAGCATCCCTGGCAAAAATGCTGCTGAGTACGAGGTCATCATAGTGATCGAGCCAGGG
  CTGCTGTTTGAGATCATGGAGATGCTCCAAGCTGAGGAGACCTCTAGCACGTCTCAGCTGAATGAGCTGA
  TGATGGCTTCCCAGACGACATTGCTGGCTCAGGAGCCACGGGAAAGAACTGCAGGTGACATTGAGCTCAC
  GGGGACATTCGTCATCAATCTAGAAGGTGGTGACATTCGTGAGGAGTCTTCATATAAAGTCATTGTGGTA
  CCGACTGCCAGAGAGAAGTGCCCTCGATGTTGGAAGCATACCTCTGAGACTGCAGACGCTCTCTGTCCTC
  GGTGTGCAGAGGTCATTGGTGCAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211440 representing BC052403
  Red=Cloning site Green=Tags(s)
  
  MHWGLCPRGPGAAAVAAAGSFWGPARLPSRLGCLGMTRRLVVRSVAGADSPQSSSKGGRYRDTVLLPQTS
  FPMKLLGRQQSDMELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFH
  MMRGSKVHFVPGWDCHGLPIETKVLSELGVDAQSLSAMEIREKARSFAQAAIEKQKSAFVRWGVMADWNN
  CYYTFDPKYEAKQLRVFYQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRSIYVRFPLLRPPP
  KLESLTDASSPVSVLVWTTQPWTIPANQAICYMPEAKYAVVKCSASGHLYILAEDKIAPVASALETTFDV
  VAAFSGVDLEGGTCSHPLTPDKVSPLLPATHVTMAKGTGLVHTAPAHGMEDYSVASQHSLPMDCLVDEGG
  MFTDAAGPELQNKAVLKEGTDVVIKMLQATKNVLKEENIVHSYPCDWRTKTPVLIRASKQWFVNITDIKA
  AAKESLKTVKFIPGAALNSMTDMLDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIIKLVEQH
  GSDVWWTLPAEQLLPAEVLAQAGGPGALEYAPGQDILDIWFDSGTSWSCVLQDTQQRADLYLEGKDQLGG
  WFQSSLLTSVATRSKAPFRTVMVHGFTLGEKGEKMSKSLGNVINPDTIISGGKDHSKEPPYGADILRWWI
  AESNVFTEVTIGPSVLSAARDDISKLRNTLRFLLGNLTGFNPETDSVPVKNMYVIDQYMLHLIQDFATKI
  TDSYKQYDFGKVVRLLKAFYTRELSSFYFSIVKDRLYCENEKDPKRRSCQTALAEILDVLVRAFAPILPH
  LAEEVFQHIPYVTEPKSVFRTGWINTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYEVIIVIEPG
  LLFEIMEMLQAEETSSTSQLNELMMASQTTLLAQEPRERTAGDIELTGTFVINLEGGDIREESSYKVIVV
  PTAREKCPRCWKHTSETADALCPRCAEVIGAK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052403
• ORF Size: 3038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052403, AAH52403
• RefSeq Size: 3499
• RefSeq ORF: 3038
• Locus ID: 381314