Ube2o (BC059193) Mouse Tagged ORF Clone

CAT#: MG211566

Ube2o (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ube2o
• Synonyms: E2-230K, mKIAA1734, 9630022H21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211566 representing BC059193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACAGAAGATGGTGCCAAACTCTACGATGTCTGCCCTCACGTCAGCGACTCGGGTCTCTTCTTTG
  ATGACTCCTATGGCTTCTACCCAGGCCAAGTCCTTATTGGCCCAGCCAAGATCTTCTCCAGTGTCCAGTG
  GCTCTCGGGGGTCAAGCCTGTACTCAGCACCAAGAGCAAGTTCCGTGTGGTGGTGGAGGAGGTGCAGGTT
  GTAGAACTGAAAGTCACGTGGATTACCAAGAGCTTCTGCCCCGGCGGCACGGACAGCGTTAGCCCCCCAC
  CTTCCATCATCACTCAGGAAAACCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCA
  GCTTGGGGAACGCTGTCTGTATGTCTTCCCAGCCAAGGTAGAGCCGGCCAAGATTGCCTGGGAATGTCCA
  GAAAAAAACTGCGCCCAGGGGGAGGGCTCTATGGCCAAGAAGGTGAAGCGTCTGTTGAAGAAGCAAGTTG
  TGAGAATCATGTCCTGCACCCCAGATACCCAGTGTCCTAGGGACCATTCCATGGAAGACCCAGACAAGAA
  AGGGGAAGCCAGAGCAGGGAGTGAAATAGGGTCTGCTAGCCCGGAGGAGCAACCTGATGGATCAGCCAGC
  CCCGTGGAGATGCAGGATGAGGGCTCGGAGGAGCTTCAGGAGACATGCGAGCCACTGCCCCCCTTCCTCT
  TGAAGGAGGGTGGAGATGACGGGCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCGGCTGACGATAC
  AGATGACACCAGCTCTGTGACCTCCTCCGCCAGCTCTACTACCTCCTCCCAGAGTGGCAGTGGCACTGGT
  CGTAAAAAGAGCATTCCTTTGTCCATCAAAAACTTGAAGCGAAAACACAAGAGGAAGAAGAATAAGGTCA
  CCCGTGACTTCAAGCCTGGAGACAGGGTGGCAGTGGAGGTTGTGACCACGATGACCTCAGCTGATGTGAT
  GTGGCAAGATGGCTCTGTGGAATGCAACATCCGCTCCAATGACCTTTTCCCTGTGCACCACCTGGACAAC
  AATGAGTTCTGCCCTGGGGACTTCGTGGTGGACAAGCGAGTCCAGAGCTGCCCAGACCCTGCTGTCTATG
  GTGTGGTGCAGTCTGGGGATCATGTTGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGCGACCAAGTGG
  GGATGACGTGGAGCTCATTGGAGAAGAGGAGGACGTGAGCGTCTATGACATTGCTGACCACCCTGACTTT
  CGCTTCCGCACAACTGACATTGTCATCCGCATTGGTAACACGGAGGATGGGGCTCTACCTAAGGAGGATG
  AGCCCTCAGTGGGCCAAGTGGCCCGAGTGGATGTCAGCAGCAAAGTGGAAGTGGTGTGGGCTGACAACTC
  GAAAACCATCATCCTGCCACAGCACTTATACAACATCGAGTCTGAGATTGAGGAGTCTGACTACGACTCC
  GTGGAAGGCAGCTCCAGTGGGGCCTCCTCGGATGAGTGGGAGGACGACAGCGACAGCTGGGAGACAGACA
  ACGGGCTCGTGGACGATGAGCACCCCAAGATAGAGGAGCTGGCCGCCATCCTGCCTGCCGAGCAGCCCAC
  TGCCCCCGAGGAGGACAAGGGCGTGGTCATCAGTGAGGAGGCGGCCACGGCTGCCATCCAGGGCGCCGTG
  GCCATGGCTGCCCCCGTGGCAGGGCTGATGGAGAAAGCTGGGAAGGACGGGCCTCCCAAGAGTTTCCGGG
  AGCTGAAGGAGGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTCACAGGCTC
  GCCCACCTCGCCCACCGTGGAGCCTGAGAAGCCAACCCGAGAGAAGAAGTTTTTGGATGATATCAAGAAG
  CTACAGGAGAACCTCAAGAAGACCCTGGACAATGTGGCCATTGCTGAGGAGGAGAAGATGGAGGCAGTAC
  CCGACACAGAGCGCAAGGAGGAAAAGCCTGAGGTGCAGTCCCCAGTGAAAGCCGAGTGGCCCAGTGAGAC
  ACCTGTGCTCTGTCAGCAGTGCGGTGGCAGGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTC
  TCTGTGCTGGAGTTTGCACCCTCAAACCACTCTTTCAAGAAGATTGAGTTCCAGCCCCCGGAAGCCAAGA
  AGTTCTTCAGCACAGTGCGGAAGGAGATGGCTCTGCTGGCCACCTCGCTGCCCGATGGCATCATGGTCAA
  GACTTTTGAGGACAGAATGGACCTCTTCTCTGCACTGATCAAGGGCCCCACTCGGACTCCCTATGAAGAC
  GGCCTCTACCTATTTGACATCCAGCTTCCCAACATCTACCCTGCGGTGCCCCCCCACTTTTGCTACCTCT
  CCCAGTGCAGTGGCCGCCTGAACCCTAACCTGTATGACAATGGGAAAGTGTGTGTCAGCCTCCTAGGTAC
  CTGGATTGGAAAGGGGACAGAGAGGTGGACAAGCAAGTCTAGCCTTCTCCAGGTGCTCATCTCCATCCAA
  GGTCTGATCCTGGTGAATGAACCATACTACAATGAAGCTGGCTTTGACAGTGACCGGGGCCTGCAGGAAG
  GCTATGAGAACAGTCGCTGCTACAACGAGATGGCATTAATCCGTGTGGTGCAGTCCATGACCCAGTTGGT
  GCGACGACCTCCTGAGGTCTTCGAACAGGAGATTCGGCAGCACTTCAGTGTGGGTGGCTGGCGGCTGGTG
  AACCGAATTGAGTCCTGGCTGGAAACCCATGCCATGCAGGAGAGGGCCCAGGTGATGCCCAATGGGGCCC
  CTAAAGACAGCAGCTCTCTGGAGCCCATGGCTGCAGCTGAACTCTCAGACTCCGGCCGTGAAGAGCCTGA
  AGATGTTGGGATGGCCCCTGGGGAGGCGTCCCAGGGCTCAGACTCAGAGGGTGGAGCCCAGGGCCCTGCC
  TCAGCCAGCAGAGACCACACAGAGCAGACTGAGACAGCCCCTGATGCATCCGCACCTCCCAGCGTTAGAC
  CAAAGAGGAGGAGGAAGAGCTACCGCAGCTTCCTGCCTGAGAAGAGCGGTTACCCTGACATCGGCTTCCC
  CCTCTTCCCACTCTCCAAGGGTTTCATCAAGAGCATCCGGGGGGTCCTGACACAGTTCCGTGCTGCCCTG
  CTGGAGGCGGGCATGCCGGAAAGCACAGAGGACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211566 representing BC059193
  Red=Cloning site Green=Tags(s)
  
  MNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQV
  VELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECP
  EKNCAQGEGSMAKKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSAS
  PVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTG
  RKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDN
  NEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDF
  RFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDS
  VEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGAV
  AMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKK
  LQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVF
  SVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYED
  GLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQ
  GLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLV
  NRIESWLETHAMQERAQVMPNGAPKDSSSLEPMAAAELSDSGREEPEDVGMAPGEASQGSDSEGGAQGPA
  SASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAAL
  LEAGMPESTEDK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC059193
• ORF Size: 3186 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC059193.1
• RefSeq Size: 4569
• RefSeq ORF: 3188
• Locus ID: 217342