Ube3b (NM_054093) Mouse Tagged ORF Clone

CAT#: MG211580

Ube3b (GFP-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ube3b
• Synonyms: AI449831; AU020130
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211580 representing NM_054093
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACTGTATCTCAGACCTCCAGAGCATGGTTCATCGACAGAGCCCGGCAGGCACGAGAAGAGAGGC
  TGGTGCAGAAGGAGCGGGAGCGCTCAGCGGTGACGATCCAGGCCCTAGTCCGCAGTTTCCTCTGCCGGAG
  GCGACTGCACAGAGACATCAGGAAAGAGATTGATGAGTTTTTTAGTGCTGATGAGTCTGGGTCCAGTAAA
  AGAAGTGCACTTTGTATTTTTAAGATTGCCAGGAGACTGCTGTTTATATGCAAAACTACCGAGGACAGCG
  AGAGACTGGAGAAGTTGTGTCGAAGCATCCTGAACAGCATGGATGCCGAGAATGAGCCAAAGGTGTGGTA
  CGTGTCCCTGGCTCTCTCCAAGGACCTCACGCTGCTGTGGATTAAGCAGATCAAGAGCATCCTGTGGCAC
  TGCTGTGAGCTTCTGGGGCAGCTCAAGCCAGAGATCTTACAGGACTCCAGGCTCATCACGCTGTACCTCA
  CCATGCTTGTGACCTTCACAGACACTTCAACATGGAAGATTCTCCGGGGAAAAGGTGAGAGCCTTCGGCC
  AGCCCTGAACCACATTTGCGCAAATATAATGGGCCATCTCAACCAGCGTGGACTTTACTCTGTATTGCAG
  GTACTGCTGACCCGAGGCCTGGCCAGGCCCCGGCCCTGTCTGTCCAAAGGCATGTTAACAGCAGCCTTCT
  CTCTGGCCCTGCGCCCTGTGGTTGCTGCGCAGTTCTCAGACAATCTTATGCGACCATTCATCATCCACGT
  CATGTCGGTACCTGCTCTCGTGGCTCATCTCAGCACCGTGGCCCCTGAGCGCCTCGGCGTTTTAGAGTCC
  CATGACATGCTTCGTAAATTCATCGTCTTCCTGAGAGATAGAGACCGATGCCGCGATGCTTGTGAGAGTT
  TAGAAGGCTGCCATACCCTGTGTCTCATGGGCAATCTCCTGCACTTGGGCTCCCTCAGCCTCAGGCTGTT
  AGAGGAAGAGATGGATGGGTTTGTGAGCGCGCTCACGCAGATGCTGTGCTACTGTCAGAAGTACGTGGCT
  CAGAAGAAGTCCAACCTGACCCACTGGCACCCTGTGCTCGGCTGGTTTTCCCAACCTGTGGATTACGGCC
  TCAATGATTCCATGTACCTGATCACCAAGCAGCTGCAGTTCCTGTGGGCGGTGCCTCTCATCCGCATTCT
  CTTCAGTGACATCCTGAGCAGGAAGCTGCTGGAGCATGCAGAGCCGGCCCCGGTGCAACCGCAGCCGTCC
  TCACCGCAGACGGTGCTCCCCGTGAAGAGCCTCCTCAAGCGTGCGTTTCAGAAGTCGGCTTCGGTTCGGA
  ACATCCTCAGGCCTGTTGGGGGCCGCCGTGTCGACTCCGCTGAGGTGCGGAAGGTCTGCAACATTTGCGT
  GCTCTACCAGACCTCGCTGACGACCCTCACCCAGATCCGCCTGCAGATCCTCACAGGTCTTACGTACCTG
  GATGACCTGCTGCCCAAGCTCTGGGCGTTTATCTGTGAGCTGGGGCCCCACGGCGGGCTCAAGCTCTTCC
  TGGAGTGCCTGAACAATGACACTGGAGAGTCCAAGCAGCTCCTGGCCATGCTGATGCTCTTCTGTGACTG
  TTCCCGGCACCTCATCACCATCCTCGATGACATTGAAGTTTATGAAGAACAGATCTCCTTCAAGCTGGAA
  GAGCTGGTCACCATCTCCTCGTTCCTCAATTCCTTCGTGTTTAAGATGATCTGGGACGGAATTGTAGAGA
  ATGCCAAGGGTGAGACCTTGGAGCTGTTCCAGTCCGTCCATGGCTGGCTGATGGTGCTGTATGAGCGAGA
  CTGCCGCCGGCGCTTTGCTCCGGAGGACCACTGGCTACGGAGGGACCTCAAGCCTGGTGTGCTCTTCCAG
  GAGCTCGACAAGGACCGAAGGAGGGCTCAGCTGGTCCTGCAGCACATCCCCCACGTCGTGCCTCACAAGA
  ACAGAGTTCTTCTGTTCCGGAACATGGTTATCAAGGAGAAAGAGAAGCTGGGGCTTGTGGAGACCAGCTC
  TGCCTCTCCTCACGTCACCCACATCACCATCCGAAGGTCCCGGATGTTGGAGGACGGCTACGAACAGCTC
  CGGCAGCTTTCCCAGCATGCCATGAAGGGTGTGATCCGAGTGAAGTTTGTCAACGACCTCGGGGTGGACG
  AGGCTGGGATTGACCAGGATGGTGTCTTCAAGGAGTTTCTGGAAGAGATCATCAAGAGGGTGTTTGACCC
  CGCACTCAACCTGTTCAAGACAACCAGCGGGGATGAGCGGCTGTACCCATCACCCACGTCCTACATTCAC
  GAGAATTACCTGCAGCTCTTCGAGTTTGTGGGGAAGATGCTGGGAAAGGCAGTGTATGAGGGGATCGTGG
  TAGATGTGCCCTTTGCCTCCTTCTTCCTGAGCCAGATGCTCGGGCACCACCACAGCGTCTTCTACAGCTC
  CGTGGATGAGCTTCCCTCCCTGGACTCGGAGTTTTATAAAAACCTCACCTCCATCAAGCGCTACGATGGA
  GACATCGCAGACCTCGGGCTGACGCTGTCTTACGATGAAGACGTCATGGGTCAGCTCGTCTGCCACGAAC
  TGGTTCCCGGAGGGAAGACCATCCCGGTTACAGATGAGAACAAAATTAGCTACATCCATCTGATGGCGCA
  TTTCCGAATGCACACTCAAATTAAAAACCAGACAGCTGCTCTTATCAGTGGCTTCCGTTCCATCATCAAG
  CCCGAGTGGATCCGAATGTTCTCTACCCCTGAGCTTCAGCGCCTCATCTCCGGGGACAACGCTGAGATAG
  ATCTGGAAGACTTAAAGAAGCACACAGTGTACTACGGTGGCTTTCATGGGAGCCACAGGGTCATCATCTG
  GCTCTGGGATATCCTGGCTTCCGACTTCACGCCTGAAGAAAGAGCCATGTTCCTGAAGTTTGTGACCAGC
  TGCTCCCGGCCCCCGCTCCTAGGATTCGCCTACCTCAAGCCTCCGTTCTCCATCCGCTGTGTGGAAGTAT
  CAGATGACCAGGACACTGGGGATACCCTGGGCAGCGTCCTCAGGGGCTTCTTCACCATCCGCAAGAGGGA
  GCCAGGGGGGCGTCTGCCCACATCTTCCACTTGCTTCAACCTGCTTAAGCTGCCCAACTACAGCAAGAAG
  AGCGTACTGCGAGAGAAACTACGCTATGCCATCAGCATGAACACGGGCTTTGAGCTCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211580 representing NM_054093
  Red=Cloning site Green=Tags(s)
  
  MFTVSQTSRAWFIDRARQAREERLVQKERERSAVTIQALVRSFLCRRRLHRDIRKEIDEFFSADESGSSK
  RSALCIFKIARRLLFICKTTEDSERLEKLCRSILNSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWH
  CCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGLYSVLQ
  VLLTRGLARPRPCLSKGMLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLES
  HDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVA
  QKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPS
  SPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYL
  DDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLE
  ELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQ
  ELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQL
  RQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIH
  ENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDG
  DIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIK
  PEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTS
  CSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKK
  SVLREKLRYAISMNTGFELS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_054093
• ORF Size: 3210 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_054093.2, NP_473434.2
• RefSeq Size: 5191
• RefSeq ORF: 3213
• Locus ID: 117146