Slc12a4 (NM_009195) Mouse Tagged ORF Clone

CAT#: MG211608

Slc12a4 (GFP-tagged) - Mouse solute carrier family 12, member 4 (Slc12a4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc12a4
• Synonyms: AW546649; KCC1; RBCKCC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211608 representing NM_009195
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCACTTCACCGTGGTGCCGGTGGACGGGCCGCGACGCGGCGACTATGACAACCTCGAGGGGCTCA
  GTTGGGTGGACTACGGCGAGCGCGCCGAGCGGGAAGACTCGGACGGACAGGGTAACCACAGAGAGAATAG
  CCCTTTCCTTTGCCCTTTGGACGCCTCCAGAGGAAATGACTACTATGACCGGAACTTGGCACTGTTTGAG
  GAGGAGCTGGACATCCGCCCAAAGGTGTCATCTCTCCTGGGCAAGCTTGTCAGCTATACCAACCTCACCC
  AAGGAGCCAAGGAGCACGAGGAGGCTGAGAGTGGAGAAGGTGGCCGACGGAGAGCTGCCAAGGCTCCCAG
  CATGGGCACACTCATGGGAGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTCATCCTCTTCCTGCGG
  CTGACCTGGATGGTGGGCACAGCTGGTGTACTGCAGGCTCTCCTCATTGTCCTCATCTGCTGCTGCTGTA
  CCCTGCTGACAGCCATCTCCATGAGCGCCATTGCCACCAACGGCGTGGTTCCAGCTGGTGGCTCGTACTT
  CATGATTTCCCGCTCTTTGGGACCAGAATTCGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGGACGACA
  TTTGCAGCAGCCATGTATATCCTAGGGGCCATTGAGATCTTGCTGACCTACATTGCTCCGCCAGCTGCCA
  TCTTTTACCCATCGGGCACCCATGACATGTCAAGCGCCACCTTGAATAACATGAGAGTGTATGGGACCAT
  TTTCCTGACTCTGATGACCCTAGTGGTGTTTGTCGGTGTCAAGTACGTGAACAAGTTCGCCTCGCTCTTC
  CTGGCCTGCGTGATCATCTCCATCCTTTCCATTTACGCGGGAGGCATCAAGTCCATTTTTGACCCTCCCG
  TGTTTCCGGTGTGTATGCTGGGCAATAGGACCCTGTCTCGGGACCAGTTTGACATCTGTGCCAAGACAGT
  CGTGGTGGACAATGAGACAGTGGCCACCCGGCTGTGGACTTTCTTCTGCCACAGCCCCAACCTTACTGCT
  GACTCCTGTGACCCCTACTTCCTGCTCAACAATGTGACAGAGATTCCTGGCATACCTGGGGCAGCTGCTG
  GTGTGCTCCAGGAGAACCTCTGGAGCGCTTACCTGGAGAAGGGGGAGGTCGTGGAGAAGCATGGGCTGCC
  GTCCACAGACACCCTCGGCCTAAAGGAGAGCCTGTCCCTGTATGTGGTGGCTGACATCGCCACGTCCTTC
  ACCGTGCTGGTTGGCATCTTTTTCCCTTCTGTGACAGGCATCATGGCTGGTTCAAACCGCTCCGGGGATC
  TCCGTGACGCCCAGAAGTCTATCCCAGTGGGGACCATTCTGGCCATTGTTACCACTTCGCTGGTGTACTT
  CAGCAGTGTGATCCTCTTCGGTGCCTGCATCGAGGGTGTGGTACTCCGGGACAAGTACGGTGATGGCGTC
  AGCAGGAACCTGGTGGTGGGCACCCTGGCCTGGCCTTCGCCTTGGGTCATCGTGGTTGGCTCCTTCTTCT
  CAACATGTGGGGCTGGTCTCCAGAGCCTCACTGGGGCGCCACGTTTACTGCAAGCCATTGCCAAGGATAA
  CATCATCCCCTTCCTCCGGGTGTTTGGCCATGGGAAAGCAAACGGTGAACCGACGTGGGTCCTCCTCCTG
  ACAGCGCTCATCGCTGAGCTGGGCATCCTGATTGCTTCCCTTGACATGGTGGCCCCCATTCTGTCCATGT
  TCTTTCTGATGTGTTACCTCTTTGTAAACTTGGCCTGTGCCGTGCAGACACTTCTGAGGACCCCCAACTG
  GCGGCCCCGGTTCAAGTACTATCACTGGACATTGTCTTTCCTAGGCATGAGTCTCTGCCTGGCTCTTATG
  TTTGTCTCCTCCTGGTACTATGCCCTAGTGGCCATGCTCATCGCAGGCATGATCTACAAGTACATCGAGT
  ACCAAGGGGCCGAGAAGGAGTGGGGCGATGGGATCCGAGGCCTGTCCCTGAGTGCGGCACGCTATGCACT
  GCTGAGGCTGGAGGAAGGGCCTCCTCACACCAAGAACTGGCGGCCTCAGCTCCTGGTGCTGCTTAAGTTA
  GACGAAGATCTTCATGTGAAGTACCCCCGGCTCCTCACCTTCGCCTCCCAGCTGAAGGCCGGGAAGGGCC
  TGACAATCGTTGGCTCTGTCATCCAGGGCAGCTTTCTGGAGAGCTATGGTGAAGCCCAGGCCGCTGAGCA
  GACCATCAAGAACATGATGGACATTGAGAAAGTCAAAGGCTTCTGCCAGGTAGTGGTGGCCAGCAAGGTT
  CGAGAGGGACTGGCCCACCTCATCCAGTCTTGCGGCCTGGGCGGCATGAGACACAACTCCGTGGTGCTGG
  GCTGGCCCTATGGCTGGCGACAGAGTGAGGACCCACGTGCCTGGAAGACCTTTATTGACACTGTGCGCTG
  CACCACAGCTGCCCACCTGGCCCTGCTGGTGCCAAAGAACATAGCTTTCTACCCCAGCAACCACGAGCGC
  TACCTGGATGGCCATATCGACGTGTGGTGGATCGTGCATGATGGAGGCATGCTTATGCTGCTGCCCTTTC
  TGCTGCGCCAGCATAAGGTTTGGAAGAAGTGCCGGATGCGCATTTTCACCGTGGCCCAGATGGATGACAA
  CAGCATCCAGATGAAGAAGGATCTGGCCATCTTCCTGTATCACCTCCGCCTGGAAGCTGAAGTGGAGGTG
  GTAGAGATGCACAACAGTGACATCTCTGCCTATACCTATGAGCGGACACTGATGATGGAGCAGCGGTCTC
  AGATGCTGCGGCAGATGAGGTTGACCAAAACTGAGCGGGATCGAGAGGCCCAGCTGGTAAAGGACAGGCA
  CTCGGCTCTTAGGCTGGAGAGCCTCTACTCCGACGAGGAGGAAGAGTCTGTGGCAGGGGCCGACAAGATC
  CAGATGACGTGGACCAGAGACAAGTACATGGCTGAGCCCTGGGACCCCAGCCACGCCCCTGACAACTTCC
  GGGAGCTGGTGCACATTAAGCCGGACCAGTCCAACGTGCGGCGCATGCACACTGCTGTGAAGCTCAACGA
  GGTCATTGTCACGCGCTCCCACGATGCCCGTCTGGTTTTGCTGAACATGCCTGGCCCCCCTAAGAACAGC
  GAGGGCGACGAGAACTACATGGAATTCCTTGAAGTCCTAACCGAGAGCCTTGAACGGGTGTTGTTGGTGC
  GTGGCGGCGGCCGGGAAGTCATCACCATCTATTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211608 representing NM_009195
  Red=Cloning site Green=Tags(s)
  
  MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEREDSDGQGNHRENSPFLCPLDASRGNDYYDRNLALFE
  EELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLR
  LTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTT
  FAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLF
  LACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTA
  DSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSF
  TVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGV
  SRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWVLLL
  TALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALM
  FVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKL
  DEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKV
  REGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHER
  YLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEV
  VEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEEESVAGADKI
  QMTWTRDKYMAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNS
  EGDENYMEFLEVLTESLERVLLVRGGGREVITIYS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009195
• ORF Size: 3823 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009195.2
• RefSeq Size: 3747
• RefSeq ORF: 3258
• Locus ID: 20498
• Gene Summary: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia. [UniProtKB/Swiss-Prot Function]