Slc12a5 (NM_020333) Mouse Tagged ORF Clone

CAT#: MG211653

Slc12a5 (GFP-tagged) - Mouse solute carrier family 12, member 5 (Slc12a5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc12a5
• Synonyms: KCC2; mKIAA1176
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211653 representing NM_020333
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAACAACCTGACGGACTGCGAGGACGGCGATGGGGGAGCCAACCCCGGTGATGGCAACCCCAAAG
  AGAGCAGTCCCTTCATCAACAGCACGGACACGGAGAAGGGCAGAGAGTACGATGGCAGGAACATGGCCCT
  GTTTGAGGAGGAGATGGACACCAGCCCCATGGTATCCTCCCTGCTCAGTGGGCTGGCCAACTACACCAAC
  CTACCCCAGGGAAGTAGAGAGCATGAAGAAGCAGAAAATAATGAGGGTGGAAAAAAGAAGCCGGTGCAGG
  CTCCTCGAATGGGCACCTTCATGGGTGTGTACCTGCCGTGCCTGCAGAACATCTTTGGTGTCATCCTCTT
  CCTGCGGCTCACGTGGGTGGTGGGCATCGCGGGCATCATGGAGTCCTTCTGTATGGTCTTCATTTGCTGC
  TCCTGTACGATGCTCACAGCCATTTCCATGAGTGCAATCGCAACCAATGGTGTTGTGCCTGCTGGTGGCT
  CGTACTACATGATTTCCAGGTCTCTGGGCCCGGAGTTTGGGGGCGCCGTGGGCCTCTGCTTCTACCTGGG
  CACCACCTTTGCTGGGGCTATGTACATCCTTGGCACGATCGAGATCCTGCTGGCTTATCTCTTCCCAGCT
  ATGGCCATCTTCAAGGCAGAAGATGCCAGTGGGGAGGCGGCCGCCATGCTGAACAACATGCGGGTGTATG
  GCACCTGTGTGCTCACCTGCATGGCCACCGTTGTCTTTGTGGGTGTCAAGTACGTCAACAAGTTTGCCTT
  GGTCTTCCTGGGTTGCGTCATCCTGTCCATCCTGGCCATCTATGCAGGGGTCATCAAGTCTGCCTTCGAC
  CCACCCAATTTCCCGATCTGCCTCCTGGGGAACCGCACGCTGTCTCGCCATGGCTTTGATGTCTGTGCCA
  AGCTGGCTTGGGAAGGAAATGAGACAGTGACCACACGGCTCTGGGGCCTTTTCTGCTCCTCCCGCCTCCT
  CAATGCCACCTGTGATGAGTACTTCACCCGAAACAATGTCACAGAGATCCAGGGCATTCCTGGTGCTGCC
  AGTGGTCTCATCAAAGAGAACCTGTGGAGTTCTTACCTGACCAAAGGGGTGATTGTCGAGAGGCGTGGGA
  TGCCCTCTGTGGGCCTGGCAGACGGTACCCCCGTAGACATGGACCACCCCTATGTCTTCAGTGATATGAC
  CTCCTACTTCACCCTGCTCGTTGGTATCTACTTCCCCTCAGTCACAGGGATCATGGCTGGCTCAAACCGA
  TCTGGAGACCTGCGGGATGCCCAGAAGTCTATCCCTACTGGAACTATCCTGGCCATTGCTACCACCTCTG
  CTGTCTACATCAGCTCTGTTGTTCTGTTTGGAGCCTGCATCGAGGGGGTCGTCTTACGGGACAAGTTTGG
  GGAAGCTGTGAATGGCAACTTGGTGGTGGGCACCCTGGCCTGGCCTTCTCCCTGGGTCATCGTCATAGGC
  TCTTTCTTCTCTACCTGTGGGGCTGGATTACAGAGCCTCACAGGGGCCCCACGTCTGCTGCAGGCCATCT
  CCCGGGATGGCATAGTGCCCTTCCTGCAGGTCTTTGGCCATGGCAAAGCTAATGGAGAGCCAACCTGGGC
  GCTGCTGCTGACTGCCTGCATCTGTGAGATCGGCATCCTCATAGCCTCCCTGGATGAGGTCGCCCCTATA
  CTTTCCATGTTCTTCCTAATGTGTTACATGTTTGTGAACTTGGCTTGTGCGGTGCAGACGCTGCTGAGGA
  CACCCAACTGGAGGCCACGATTTCGCTATTACCACTGGACTCTCTCCTTCCTGGGCATGAGCCTCTGCCT
  GGCCCTCATGTTCATTTGCTCCTGGTACTACGCACTGGTGGCCATGCTCATTGCCGGACTCATTTATAAG
  TACATCGAGTACCGGGGGGCGGAGAAGGAGTGGGGGGATGGAATCCGAGGCCTGTCTCTCAGTGCAGCAC
  GCTATGCTCTCTTGCGCCTGGAGGAAGGACCTCCGCATACGAAGAACTGGAGGCCCCAGCTGCTGGTGCT
  GGTGCGTGTGGACCAGGATCAGAACGTGGTGCATCCGCAGCTGCTCTCCCTGACCTCCCAGCTCAAGGCA
  GGGAAGGGCCTGACCATTGTGGGCTCCGTCCTTGAGGGCACCTTTCTGGACAACCATCCACAGGCTCAGC
  GGGCAGAGGAGTCTATCAGGCGCCTGATGGAGGCTGAGAAGGTGAAGGGCTTCTGCCAGGTAGTGATCTC
  CTCCAACCTGCGTGATGGTGTGTCCCACCTGATCCAGTCTGGGGGCCTCGGGGGATTGCAACACAATACC
  GTGCTGGTGGGCTGGCCTCGCAACTGGAGGCAGAAGGAGGATCATCAGACATGGAGGAACTTCATCGAAC
  TGGTCCGGGAAACTACAGCCGGCCACCTCGCCCTGCTGGTCACCAAGAATGTTTCCATGTTTCCCGGGAA
  CCCTGAGCGCTTCTCGGAGGGCAGCATTGACGTGTGGTGGATTGTGCACGACGGGGGCATGCTCATGCTG
  CTGCCCTTCCTGCTGCGACACCACAAGGTCTGGAGGAAATGCAAAATGCGGATCTTCACCGTGGCCCAGA
  TGGACGATAACAGTATCCAGATGAAGAAGGACCTGACCACGTTTCTGTACCACTTACGCATTACTGCAGA
  GGTGGAGGTGGTGGAGATGCATGAGAGCGACATCTCGGCATACACCTACGAGAAGACATTAGTAATGGAG
  CAACGATCTCAGATCCTCAAACAGATGCACCTCACCAAGAACGAGCGGGAACGGGAGATCCAGAGCATCA
  CAGACGAGTCTCGGGGCTCCATTCGGAGGAAGAATCCAGCCAACCCCCGGCTCCGCCTCAATGTTCCCGA
  AGAGACAGCGTGTGACAATGAGGAGAAGCCAGAGGAGGAGGTGCAGCTGATCCATGACCAGAGTGCTCCC
  AGCTGCCCTAGCAGCTCGCCATCTCCAGGGGAGGAGCCCGAGGGGGAGAGGGAGACAGACCCAGAGGTGC
  ATCTTACCTGGACCAAGGATAAGTCAGTGGCAGAGAAGAATAAAGGCCCCAGTCCCGTCTCCTCCGAGGG
  CATCAAGGACTTCTTCAGCATGAAGCCGGAGTGGGAAAACTTGAACCAGTCCAATGTACGGCGCATGCAC
  ACAGCTGTGCGGCTGAACGAGGTCATCGTGAATAAATCTCGGGATGCCAAGCTAGTTTTGCTCAACATGC
  CCGGGCCTCCCCGCAACCGCAATGGGGATGAAAACTACATGGAATTCTTGGAGGTCCTCACTGAGCAACT
  GGACCGGGTGATGCTGGTCCGCGGTGGCGGCCGAGAGGTCATCACCATCTACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211653 representing NM_020333
  Red=Cloning site Green=Tags(s)
  
  MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTN
  LPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICC
  SCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPA
  MAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFD
  PPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAA
  SGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNR
  SGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIG
  SFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPI
  LSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYK
  YIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKA
  GKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNT
  VLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLML
  LPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVME
  QRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANPRLRLNVPEETACDNEEKPEEEVQLIHDQSAP
  SCPSSSPSPGEEPEGERETDPEVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMH
  TAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020333
• ORF Size: 3345 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020333.2, NP_065066.2
• RefSeq Size: 6042
• RefSeq ORF: 3348
• Locus ID: 57138