Sema5b (BC052397) Mouse Tagged ORF Clone

CAT#: MG211660

Sema5b (GFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sema5b
• Synonyms: SemG, mKIAA1445
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211660 representing BC052397
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTCCCAGGACCCCTGGCTCTCTCGCTGTTGCTGTCCAGCCTCACCCTGCTGGTGTCCCACCTCT
  CCAGCTCCCAGGACATTGCCAGTGAGTCCAGCAGTGAGCAACAGATGTGCACGCGGAGGGAGCACCCCAT
  CGTGGCCTTTGAAGATCTGAAGCCGTGGGTCTTTAACTTCACCTACCCTGGAGTCCGGGATTTCTCCCAG
  CTTGCTCTTGATCCCTCGAGGAATCAGCTCATCGTGGGAGCCAGGAACTACCTCTTCAGACTCAGCCTCG
  CCAATGTCTCCCTCCTTCAGGCTACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAGAGCAA
  AGGGAAAACGGAGGAGGAGTGTCAGAACTATGTACGAGTCCTGATTGTTTCCGGCCGGAAGGTGTTCATG
  TGCGGTACCAATGCCTTTTCCCCAGTGTGCTCCAGCAGACAGGTAGGGAACCTCAGCCGGACTATTGAGA
  AGATCAACGGTGTGGCCCGGTGCCCCTATGACCCACGCCACAACTCTACAGCCGTCATCTCCTCTCAGGG
  GGAGCTCTATGCAGCCACAGTCATTGACTTCTCCGGTCGGGACCCAGCCATCTACCGCAGCCTGGGCAGT
  GGGCCACCGCTTCGCACTGCCCAGTATAACTCCAAATGGCTCAATGAGCCAAATTTTGTGGCAGCCTTTG
  ACATCGGGCTGTTTGCGTATTTCTTCCTTCGGGAGAATGCCGTGGAGCATGATTGTGGGCGCACTGTGTA
  CTCTCGGGTGGCCCGGGTGTGCAAGAATGATGTAGGTGGCCGTTTCCTGCTGGAGGACACGTGGACCACA
  TTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGAGAGGTCCCCTTCTACTATAATGAGCTGCAGAGTG
  CCTTCCATCTGCCCGAGCAGGACCTCATCTATGGCGTCTTCACCACTAACGTAAACAGCATTGCGGCTTC
  TGCTGTCTGCGCCTTCAACCTCAGTGCCATCTCCAAGGCTTTCAATGGCCCATTTCGTTACCAGGAAAAC
  CCCAGGGCTGCCTGGCTCCCAATCGCCAATCCCATCCCCAATTTCCAGTGTGGCACTCTGCCGGAGACTG
  GCCCCAACGAGAACCTCACGGAGCGCAGCCTGCAGGACGCACAGCGGCTCTTCCTGATGAGCGAAGCTGT
  GCAGCCAGTGACACCAGAGCCCTGTGTCACCCAGGACAGCGTCCGCTTCTCACATCTCGTGGTAGACCTT
  GTGCAAGCTAAGGACACGCTCTATCACGTACTCTACATAGGCACGGAGTCGGGTACCATCCTGAAAGCGC
  TGTCCACGGCCAGCCGCAGCCTCCGTGGCTGCTACCTGGAGGAGCTACATGTGCTGCCTCCTGGGCGCCT
  TGAACCTCTGCGGAGCCTGCGCATCCTGCACAGCGCGCGTGCGCTCTTCGTGGGATTGAGCGACAGGGTG
  CTGCGGGTCCCACTGGAGAGGTGTTCGGCCTATCATAGCCAGGGGGCATGCCTGGGAGCACGGGACCCAT
  ACTGCGGCTGGGATGGGAAGCGGCAACTTTGCAGCACGCTTGAAGACAGTTCCAACATGAGCCTGTGGAT
  CCAGAACATCACAACCTGTCCTGTACGAAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGG
  AAACCGTGTGAGCACTTAGATGGAGACAACTCGGGTTCTTGCCTGTGCCGGGCCAGATCCTGTGACTCCC
  CAAGGCCTCGCTGTGGGGGCCTCGAGTGCCTGGGGCCATCCATCCATATTGCCAATTGCTCCAGGAATGG
  GGCGTGGACCGCATGGTCATCGTGGGCTCAGTGCAGCACGTCCTGTGGGATCGGCTTCCAGGTCCGTCAG
  CGAAGTTGCAGCAACCCGGCGCCCCGCCATGGGGGCCGCATTTGCGTGGGCAAGAGCCGGGAGGAGCGGT
  TCTGTAATGAAAATACACCTTGCCCAGTGCCCATCTTCTGGGCTTCCTGGGGTTCCTGGAGCAAGTGCAG
  CAACAACTGTGGAGGCGGCGTGCAGTCGCGACGCCGTTCTTGCGAGAATGGCAACTCGTGCCCGGGTTGC
  GGCGTGGTGAGGTCGCGGGGCTGGGGCGCGGGGAGCGGGGAGACCTACAGTCCAGGCGGGGTTGGGGGGT
  CTGAAGCCACCCTCACCCCGAAGGAGTTCAAGACCTGCAACCCCGAGGCTTGCCCGGAAGTGCGACGCAA
  CACACCCTGGACGCCCTGGCTGCCCGTGAACGTGACCCAGGGTGGAGCGCGCCAGGAGCAGCGATTCCGC
  TTCACCTGCCGCGCGCCGCTGCCGGACCCGCACGGTCTGCAGTTCGGCAAGAGGAGGACAGAGACCAGGA
  CTTGCCCGGCAGACGGCACCGGAGCCTGCGACACCGACGCCCTGGTGGAGGATCTCCTGCGCAGCGGGAG
  CACGTCACCACACACTCTGAACGGAGGCTGGGCCACCTGGGGCCCGTGGTCATCCTGCTCCCGGGACTGC
  GAGCTGGGCTTCCGCGTCCGCAAGAGAACTTGTACCAACCCGGAGCCTCGCAACGGGGGCTTGCCCTGCG
  TGGGAGACGCTGCGGAGTACCAAGACTGCAACCCACAGGCTTGCCCAGTGCGGGGTGCTTGGTCCTGCTG
  GACCGCATGGTCCCAGTGCTCAGCATCCTGCGGTGGTGGCCACTATCAACGCACCCGTTCCTGCACCAGC
  CCCGCCCCATCCCCAGGTGAGGACATCTGCCTCGGCCTGCACACGGAGGAGGCCCTATGTTCAACACAGG
  CCTGCCCAGAAGGCTGGTCACTGTGGTCTGAGTGGGGTGTCTGCACTGAGGATGGGGCCCAGAGCCGGAG
  CCGGAGCTGTGAGGAGCTTCTCCCAGGACCAGGTGCCTGTGTTGGCAACAGCAGCCAGAGCCGGCCCTGC
  CCCTACAGTGAGATTCCTGTCATCCTACCTGCTTCCAGTGTGGAGGAGACCACCAGCTGTGGAGGGTTCA
  ATCTCATCCACCTGATAGTCACTGGTGTGTCCTGCTTCCTGGTTTCTGGGCTCTTGACCTTGGCAGTGTA
  CCTGTCTTGCCAGCACTGCCAGCGCCAGTCTCAGGAGTCCACGCTTGTCCATCCTGCCACACCTAACCAC
  TTGCACTACAAGGGTGGGGGCACCCCCAAGAATGAGAAGTACACCCCTATGGAATTCAAGACACTGAACA
  AGAACAACTTAATCCCTGATGACAGAGCCAACTTCTACCCACTGCAGCAGACCAATGTGTATACAACCAC
  GTACTACCCCAGCCCACTGAACAAGCCCAGCTTCCGGCCTGAGGCCTCACCTGGACAGCGCTGTTTCCCC
  AACAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211660 representing BC052397
  Red=Cloning site Green=Tags(s)
  
  MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQ
  LALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFM
  CGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGS
  GPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTT
  FMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQEN
  PRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDL
  VQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRV
  LRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPW
  KPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQ
  RSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQSRRRSCENGNSCPGC
  GVVRSRGWGAGSGETYSPGGVGGSEATLTPKEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFR
  FTCRAPLPDPHGLQFGKRRTETRTCPADGTGACDTDALVEDLLRSGSTSPHTLNGGWATWGPWSSCSRDC
  ELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTS
  PAPSPGEDICLGLHTEEALCSTQACPEGWSLWSEWGVCTEDGAQSRSRSCEELLPGPGACVGNSSQSRPC
  PYSEIPVILPASSVEETTSCGGFNLIHLIVTGVSCFLVSGLLTLAVYLSCQHCQRQSQESTLVHPATPNH
  LHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFP
  NS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052397
• ORF Size: 3366 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052397.1
• RefSeq Size: 4658
• RefSeq ORF: 3368
• Locus ID: 20357
• Gene Summary: May act as positive axonal guidance cues. [UniProtKB/Swiss-Prot Function]